30 results about "Antibody negative" patented technology

The invention relates to the technical field of porcine pseudorabies viruses and especially relates to a gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G and a use thereof. The gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G has the accession number of CGMCC No.7957. The invention discloses the use of the gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G in vaccine preparation. After the New Zealand big white rabbit is inoculated with the 106.0TCID50 recombinant viruses, clinical symptoms such as pruritus are not caused. An oil-in-water inactivated vaccine prepared from the gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G is injected into a piglet and after four weeks, the BELISA antibody is produced but the gE antibody does not exist, and the immunization protection efficiency is 100%. After immunization on sows, the piglets produced by the sows get immunization protection and the efficiency of PRV variant virus and traditional virus immunization protection is 100%. It is proved that the ZJ011G recombinant virus has good immunogenicity and can be used for vaccine preparation.

The invention discloses a kit of chemiluminescent immunological analysis measurement of a Treponema pallidum antibody, and preparation method thereof, which belongs to the technical field of immunological analysis medical diagnosis. The kit comprises (1) a carrier coated with a treponema pallidum specific recombination protein antigen; (2) treponema pallidum antibody negative and positive reference substances; (3) an enzyme labeled treponema pallidum specific recombination protein antigen; and (4) an enzyme acted chemiluminescent primer. Furthermore, the preparation method of the kit based on the invention comprises the following steps of (1) preparing TP antibody negative and positive reference substances; (2) labeling the TP specific recombination protein antigen with an enzyme; (3) coating with the carrier; (4) sub-packaging; and (5) assembling. The kit of the detection of the Treponema pallidum antibody has the advantages of easy and simple operation, easy popularization, high sensitivity, strong specificity, good repeatability, safety, non toxicity, and no pollution.

The invention discloses a kit for quantitative detection on an O type foot-and-mouth disease virus antibody through fluorescence immunoassay magnetic particles. The kit consists of O type foot-and-mouth disease virus antibody negative serum, O type foot-and-mouth disease virus antibody positive serum, VP1 coating magnetic beads, a biotinylation goat-anti-pig antibody, a streptavidin marking fluorescent substance, a cleaning solution and an enhancing solution. The magnetic beads used in the kit have relatively large binding areas, so that the detection range is greatly increased, the reaction time is shortened, and the sensitivity is improved. The kit has a relatively wide stimulation spectrum and a relatively narrow emitting spectrum, the cost can be reduced, and the sensitivity can be improved; compared with a conventional fluorescent substance, the kit is relatively wide in detection range and relatively good in specificity. Due to adoption of a streptavidin-biotin signal amplification system, the detection sensitivity is further improved, and the kit is relatively high in sensitivity when being compared with ELISA (Enzyme-Linked Immuno Sorbent Assay) and chemiluminiscence. Together with a full-automatic detector, on-site automatic operation can be achieved, one or more samples can be simultaneously detected, and the kit is simple, convenient and rapid to operate and low in price.

The invention provides applications of deoxyhypusine dioxygenase, namely, DOHH, or a fragment thereof in preparation of a reagent for diagnosis of anti-citrullinated protein antibody negative (ACPA-negative) rheumatoid arthritis (RA). In the method, a high-density protein chip is hybridized with RA serum to screen 35 proteins, which are candidate auto-antigens of the ACPA-negative RA. It is identified that four protein antigens, DOHH, DUSP11, PTX3 and PAGE5, have high sensitivity and specificity in ACPA-negative serum of the RA, wherein the four protein antigens are all novel auto-antigens. The method shows that the DOHH, DUSP11, PTX3 and PAGE5 can be used as diagnosis markers for the ACPA-negative RA.

The invention discloses a kit for quantificationally and qualitatively detecting smooth muscle antibodies. The kit comprises a magnetic microparticle reagent marked with streptavidin, a goat anti-human IgG storage buffer solution containing HRP marks, a biotin antigen reagent, a smooth muscle antibody calibration product, a smooth muscle antibody critical value quality control product and a smoothmuscle antibody negative quality control product, wherein the surfaces of the magnetic microparticles contain carboxyl groups; and biotin antigens in the biotin antigen reagent are marked with actin.The kit has the advantages that the detection is convenient and fast; the quantificational and qualitative detection on the smooth muscle antibodies can be realized in a short time, so that the typeand the serious degree of autoimmune liver diseases can be accurately diagnosed; and the clinic medication of a doctor is guided. The prepared kit has the advantages of high sensitivity, high specificity and high accuracy, and is applicable to a semi-automatic or fully-automatic detection system. The kit combines a chemiluminescence technology with a magnetic microparticle technology, has a high use value on clinics, and is suitable for being popularized and applied.

The invention provides an ELISPOT detection kit for detecting bovine brucellosis, the ELISPOT detection kit includes: a support medium, a capture antibody, a detection antibody, a negative control and a positive control, and a specific stimulating agent, wherein the capture The antibody is the first bovine gamma interferon monoclonal antibody, the detection antibody is the second labeled bovine interferon gamma monoclonal antibody, and the specific stimulator is brucellin Br-PPD, wherein the support medium and reagent There is a microporous filter membrane on the contact surface; the capture antibody is coated on the support medium or the capture antibody and the support medium are placed separately; and the capture antibody and the detection antibody can combine with the bovine gamma interferon for antigen-antibody binding. The monoclonal antibody secreted by the hybridoma cell line used in the kit of the present invention has the advantages of high titer, good specificity, and strong affinity with natural antigens; compared with the Brucella antibody ELISA detection kit, it can realize the The early detection of Brucella infection excludes the interference of false positives caused by cross-reactions and shows good specificity.

The present invention involves the field of pseudo-rabies virus technology. It specially involves a pseudo-pseudo-rabies disease variable and GI gene lack of virus strain PRV-ZJ011g. The preservation number is CGMCC?No.7957.Its application in the preparation of vaccines.Will 106.0?The TCID50 reorganization virus inocus the New Zealand big white rabbit, which did not cause itching and other clinical symptoms.Made into an oily vaccine, 4 weeks of GB after the piglets are immunized?ELISA antibody is positive, but GE antibody is negative, and immune protection efficiency is 100%. After the sow immunization, its offspring can obtain immune protection, which is 100%of PRV mutant poison and traditional toxic immune protection efficiency;Can be used for vaccine preparation.

The invention provides a multi-antigen enzyme linked immunosorbent assay (ELISA) kit for detecting an African swine fever virus (ASFV) antibody, belonging to the field of a biotechnology and diagnosis and research of animal-borne diseases. The multi-antigen enzyme linked immunosorbent assay kit comprises expression and purification of three types of ASFV recombined antigens, preparation of positive and negative control blood serum of an ASFV antibody, optimal envelope antigen combination and concentration determination, optimization of multi-antigen ELISA (MA-ELISA (Microalbumin-Enzyme Linked Immunosorbent Assay)) reaction parameters, determination of an ASFV antibody negative blood serum critical value, MA-ELISA detection artificial infection and determination of sensitivity, specificity and repeatability of field blood serum samples. By detecting and testifying a large quantity of known blood serum samples, the sensitivity, the specificity and the repeatability of detecting the ASFV antibody by the MA-ELISA are obviously higher than those of an ELISA method recommended by World Organization for Animal Health and oversea similar kits; and the multi-antigen enzyme linked immunosorbent assay kit can be used for ASFV serological diagnosis, epidemiological investigation and live pig import and export quarantine inspection.

The present invention relates to a method for reducing the occurrence and / or severity of viral infections. The method embodies procedures for expanding HIV from the blood of HIV antibody negative donors and deriving a non-infectious virus particle product that is antigenic. The procedures for deriving the antigenic, non-infectious virus particle product are optimally designed to maintain the integrity of the envelope proteins while maximizing the depletion of capsid proteins and RNA. The resulting virus particle product, when introduced into humans or non-human animals, enables the production of antibodies that target the natural envelope macromolecular structure that is required for infectivity. The present invention can be applied to producing virus stocks from the blood of HIV-seronegative donors, for deriving non-infectious virus particles that retain intact envelope proteins, for producing anti-viral antibodies, and for administering anti-virus antibodies to patients.

The invention discloses an ELISPOT detection kit for detecting brucellosis and an application thereof. An ELISPOT detection kit for detecting brucellosis, said ELISPOT detection kit comprising: support medium, capture antibody, detection antibody, negative control and positive control and specific stimulator, said ELISPOT detection kit Specific inhibitors are also included to detect the presence or absence of Brucella infection bidirectionally by specific stimulators and specific inhibitors. The ELISPOT detection kit of the present invention determines whether there is Brucella infection through positive and negative experiments, and the detection result is more objective and accurate, and can truly reflect the immune level in the body, so as to solve the accuracy rate existing in the current detection of Brucella infection Low, long cycle and other issues.

The invention discloses an enzyme linked immunosorbent assay (ELISA) kit for detecting encephalomyocarditis virus (EMCV) antibodies and application of the kit. The kit comprises an ELISA plate coating EMCV recombinant antigens, enzyme labeled EMCV antigen working solution, EMCV antibody positive control, EMCV antibody negative control, lotion, a color-developing agent A, a color-developing agent B and stop solution. The invention also relates to application of the kit to detecting EMCV antibodies of human and animals. The kit can detect human and animals, has high sensitivity, strong specificity, good thermal stability, low detection cost and short detection time and is suitable for detecting mass EMCV antibodies. Through tests, positivity and negativity of 99.6% of serums can be directly judged, so the kit has good practical effect.

The invention provides a serological testing method for the potency of an inactivated vaccine against duck Tembusu viral diseases. According to the method, HI antibody-negative ducks are used and divided into two groups, i.e., an immunized group consisting of ten ducks and a control group consisting of five ducks; each duck in the immunized group receives hypodermic or intramuscular injection of a vaccine against duck Tembusu viral diseases; immunization with an inactivated vaccine is carried out twice, a dosage of 0.5 ml or 1.0 ml per duck is used each time, and secondary immunization is carried out in two weeks after primary immunization; for immunization with a live vaccine, each duck is immunized according to a dosage of a standard vaccine usage amount for poultry once; in 3 to 4 weeks after inoculation, blood is acquired, serum is separated and the titer of an HI antibody is determined; and when the titer of the HI antibody in the ducks of the control group is less than 1: 5 and the titer of the HI antibody in serum of at least seven ducks of the immunized group is no less than 1: 10, it is determined that the potency of the vaccine is qualified in testing. The serological testing method provided by the invention is convenient to operate and accurate in results and can be extensively applied to potency evaluation of vaccines against duck Tembusu viral diseases and formulation of immune procedure in primary-level organizations and vaccine development and examination units.

The invention discloses a method for cleaning a pig farm to prevent diseases. Pig groups are divided into antibody negative subgroups and antibody positive subgroups according to immunity antibody detection; strengthen immunization is conducted on the antibody negative subgroups, immunity antibodies are detected again after immunization, and antibody positive pigs are brought into the antibody positive subgroups; fluid of umbilical cords of piglets is collected as detection samples when sows in the antibody positive subgroups give birth to the piglets, hog cholera virus and porcine pseudorabies virus are synchronously detected through a PCR method, and the pigs negative for both hog cholera virus and porcine pseudorabies virus are kept; regular casual inspection and comprehensive detection are conducted on the kept pigs, the pigs positive for both hog cholera virus and porcine pseudorabies virus or positive for one of hog cholera virus and porcine pseudorabies virus are weeded out, and then hog cholera virus and porcine pseudorabies virus are synchronously cleaned away. By synchronously cleaning away hog cholera virus and porcine pseudorabies virus, the method is high in efficiency, small in stress and easy and convenient to operate.