Methods of purifying epidemic diseases in pig farms

A kind of epidemic disease and pig farm technology, applied in the direction of animal husbandry, etc., can solve the problems of abortion of pregnant sows, time-consuming and complicated collection process, etc., and achieve the effect of reducing losses and easy operation

Active Publication Date: 2017-11-14
HUNAN XINNANFANG CULTURE SERVICE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing methods for purifying epidemic diseases in pig farms cannot carry out the purification work of porcine pseudorabies and swine fever simultaneously, and need to be carried out separately
In addition, due to the large weight of breeding pigs, the collection of anterior vena cava blood or tonsils is relatively complicated, and excessive stress can easily cause miscarriage in pregnant sows. At the same time, blood sample collection requires the assistance of multiple people and special equipment, and the collection process is relatively complicated. Time-consuming, collection of tonsils is prone to cross-contamination

Method used

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  • Methods of purifying epidemic diseases in pig farms
  • Methods of purifying epidemic diseases in pig farms
  • Methods of purifying epidemic diseases in pig farms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Detection of immune antibodies and preliminary grouping

[0021] Conduct a clinical appearance inspection on the breeding pigs in the whole field, and eliminate the old, weak and breeding pigs with more than 8 litters to form a hypothetical healthy breeding group.

[0022] Collect the anterior vena cava blood of the above-mentioned hypothetical healthy breeding herd to separate serum, and detect the immune antibodies of swine fever virus, porcine pseudorabies virus, porcine circovirus, and PRRS by ELISA (enzyme-linked immunosorbent assay) method (The expression of swine fever virus antibody, porcine pseudorabies virus gB antibody, porcine circotype 2 antibody and PRRS virus antibody ELISA kit developed by Wuhan Keqian Biotechnology Co., Ltd.), select four kinds of pigs that are positive for antibodies as Pigs that were successfully immunized were included in the antibody-positive group.

[0023] Judgment criteria:

[0024] Classical swine fever virus: S / P (...

Embodiment 2

[0029] Example 2: Antibody Negative Cluster Booster Immunization

[0030] According to the results of an antibody test in the embodiment, the pigs in the antibody-negative group were immunized once with the vaccine corresponding to the disease antibody whose detection was negative, and the dosage was operated according to the instructions for use of the corresponding vaccine.

[0031] One month after the corresponding vaccine was inoculated, the corresponding immune antibody test was carried out again. Based on the judgment criteria of Example 1, the negative pigs were eliminated, and the positive pigs were included in the antibody-positive group.

Embodiment 3

[0032] Example 3: Synchronous Purification of Antibody Positive Clusters

[0033] In this example, the antibody-qualified pigs of the above-mentioned antibody test results, that is, the antibody-positive grouped pigs, collected the body fluids from the broken umbilical cord of the piglets when the sows gave birth, and sent them to the laboratory. The body fluid in the umbilical cord was cut, and DNA and RNA were extracted respectively. Wherein, the extracted RNA is detected by nested RT-PCR method to detect classical swine fever virus; the extracted DNA is detected by PCR method to detect porcine pseudorabies virus. The extracted DNA is amplified by the PCR method to amplify the gD gene fragment and the gE gene fragment of the porcine pseudorabies virus, and is identified by agarose gel electrophoresis.

[0034] Positive (venomous) pigs, the sows will be eliminated immediately after weaning, the piglets will be kept in isolation, the immunity will be strengthened, the seeds w...

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Abstract

The invention discloses a method for cleaning a pig farm to prevent diseases. Pig groups are divided into antibody negative subgroups and antibody positive subgroups according to immunity antibody detection; strengthen immunization is conducted on the antibody negative subgroups, immunity antibodies are detected again after immunization, and antibody positive pigs are brought into the antibody positive subgroups; fluid of umbilical cords of piglets is collected as detection samples when sows in the antibody positive subgroups give birth to the piglets, hog cholera virus and porcine pseudorabies virus are synchronously detected through a PCR method, and the pigs negative for both hog cholera virus and porcine pseudorabies virus are kept; regular casual inspection and comprehensive detection are conducted on the kept pigs, the pigs positive for both hog cholera virus and porcine pseudorabies virus or positive for one of hog cholera virus and porcine pseudorabies virus are weeded out, and then hog cholera virus and porcine pseudorabies virus are synchronously cleaned away. By synchronously cleaning away hog cholera virus and porcine pseudorabies virus, the method is high in efficiency, small in stress and easy and convenient to operate.

Description

technical field [0001] The invention relates to the field of disease purification, in particular to a method for purifying epidemic diseases in pig farms. Background technique [0002] Swine fever, commonly known as "rotten intestinal fever", is an acute, febrile, contagious infectious disease caused by the classical swine fever virus of the family Flaviviridae, which is highly contagious and fatal. Porcine pseudorabies is one of the primary diseases of various infectious diseases in swine herds. Pigs infected with porcine pseudorabies or swine fever in large-scale pig farms directly or indirectly cause diarrhea in piglets in the farrowing room, high defect rate in nursery pigs, and respiratory diseases in medium and large pigs, resulting in high mortality and high morbidity. cause huge economic losses. [0003] At present, in large-scale pig farms, separate purification methods are adopted for swine fever and porcine pseudorabies. For the purification of swine fever, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 喻正军李增强王贵平黄岚芬李伦勇刘清钢
Owner HUNAN XINNANFANG CULTURE SERVICE CO LTD
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