Prevention and control method of porcine pseudorabies

A porcine pseudorabies prevention and control technology, applied in biochemical equipment and methods, antiviral agents, pharmaceutical formulations, etc., can solve problems such as pig herd hazards, achieve simple methods, reduce prevention and control costs, and consolidate purification results Effect

Inactive Publication Date: 2015-06-24
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] Aiming at the current problem of serious harm caused by porcine pseudorabies to pig herds, the present invention proposes a method for the prevention and control of porcine pseudorabies, mainly aimed at breeding pig farms, using the polymerase chain reaction method to detect porcine pseudo

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  • Prevention and control method of porcine pseudorabies
  • Prevention and control method of porcine pseudorabies

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Experimental program
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Embodiment 1

[0028] (1) All pigs in the breeding farm are immunized with porcine pseudorabies gene deletion vaccine;

[0029] (2) One month after the vaccine immunization in step (1), start the purification process, that is, collect blood from the breeding sow, including 5 ml of anticoagulant blood and 5 ml of whole blood, and 5 ml of semen from the breeding boar;

[0030] (3) The semen and anticoagulant blood collected in step (2) were tested for wild virus infection by conventional polymerase chain reaction, and the serum extracted from the whole blood collected in step (2) was used for immune antibody detection;

[0031] (4) Eliminate the breeding pigs with positive wild virus infection test results in step (3), and disinfect the pens; then divide the remaining breeding pigs into antibody-positive groups and antibody-positive groups one by one according to the results of immune antibody detection in step (3). Negative group, and then divide the antibody-positive group into a high-antibo...

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Abstract

The invention discloses a prevention and control method of porcine pseudorabies. Porcine pseudorabies gene-deleted vaccine immunity is carried out on all pigs in a pig breeding farm, then, purification treatment is carried out, breeding pigs with the positive wild virus infection test result are eliminated, then, remaining breeding pigs are divided into an antibody positive group and an antibody negative group, and then the antibody positive group is divided into a high antibody group and a low antibody group; vaccines are rebred into the antibody negative group and the low antibody group, then, purification treatment is carried out on breeding sows at a time every year, and purification treatment is carried out on breeding boars at a time every 6 months. The method is adopted to comprehensively prevent and control the porcine pseudorabies, the infection positive rate of the porcine pseudorabies is reduced year by year, through implementation of three years to four years, the virus detection result of the breeding boars and the breeding sows shows zero, and the pig farm obtains the obvious economic benefits and the social benefits. The method is simple and easy to implement, animals prone to infection are truly protected, the porcine pseudorabies of the pig farm can be completely and effectively purified and controlled, the PR purification effect is consolidated, and the prevention and control cost is greatly reduced.

Description

technical field [0001] The invention relates to a method for preventing and controlling porcine pseudorabies, and belongs to the technical field of animal disease immunity. Background technique [0002] Pseudorabies virus (Pseudorabies virus, PRV) It is a serious acute infectious disease caused by the acute death of newborn piglets and neurological symptoms in piglets over 4 weeks old. Pregnant sows are characterized by reproductive disorders, abortion, stillbirth and respiratory symptoms. Porcine pseudorabies has been widely prevalent in my country since the early 1990s and the incidence is relatively serious. The existence of the disease has been reported in various regions of the country. Pigs of any age can form a latent infection after being resistant to acute PRV infection. The virus can remain latent in the pig for life. Under certain conditions, the latent virus can be activated, causing recurrent infection and spreading the virus outward. The latent-activation cyc...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K39/245C12Q1/70C12Q1/68G01N33/53A61L2/16A61P31/22A61L101/32
Inventor 舒相华宋春莲李文贵高利波白卫兵
Owner YUNNAN AGRICULTURAL UNIVERSITY
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