The invention discloses an automatic taking and using device and method for a urine cup in the urine testing process, and relates to the technical field of urine detection. The automatic taking and using device comprises a shell, a clamping mechanism, a supporting mechanism, a centering mechanism, a laser coder, a conveying mechanism and a card swiping device; and the shell includes a vertical shell and a horizontal shell which are integrally formed, the clamping mechanism, the supporting mechanism and the centering mechanism are all installed in the vertical shell, the card swiping device isinstalled outside the vertical shell, and the conveying mechanism is installed inside the horizontal shell. By means of the automatic taking and using device and method, bar code or two-dimensional code information matched with identity information of a patient is printed on the urine cup before the urine cup is taken and used, the process of manual labeling by testing staff is omitted, the condition that one cup is provided for one person is ensured, and the accuracy of detection information is ensured. In addition, whole-process automation and intelligentization of the taking and using process of the urine cup are achieved, the step of manual intervention is reduced, and the probability of pollution to the urine cup is reduced.