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Full-automatic ELISA detection device for treponema pallidum on micro-fluidic chip and detection method thereof

A microfluidic chip and Treponema pallidum technology, applied in the direction of analysis materials, instruments, etc., can solve the problems of false negative results, low sensitivity of serum/whole blood, etc., and achieve automatic control, low cost, and space occupation small effect

Active Publication Date: 2016-01-20
QIQIHAR MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method has disadvantages such as non-specific primers, low sensitivity to serum / whole blood, easily inhibited by substances such as tissue and cell debris in the specimen, and prone to false negative results.

Method used

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  • Full-automatic ELISA detection device for treponema pallidum on micro-fluidic chip and detection method thereof
  • Full-automatic ELISA detection device for treponema pallidum on micro-fluidic chip and detection method thereof

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Embodiment Construction

[0054] like figure 1 and figure 2 As shown, a fully automatic ELISA detection device for Treponema pallidum on a microfluidic chip, including a microfluidic chip 1, a DC power supply 2, a fluorescence detection system 3 and a control system 4,

[0055] The material of the cover plate of the microfluidic chip 1 is PDMS, and the substrate of the microfluidic chip 1 is a glass slide,

[0056] The microfluidic chip 1 includes a liquid storage pool I1-1, a liquid storage pool II1-2, a liquid storage pool III1-3, a liquid storage pool IV1-4, a liquid storage pool V1-5, a liquid storage pool VI1-6, Reservoir VII1-7 and main channel 1-8, said reservoir I1-1, said reservoir II1-2, said reservoir III1-3, said reservoir IV1-4 and said reservoir Said liquid reservoir V1-5 communicates with the inlet of said main channel 1-8 through sample inlet channel 1-9 respectively, and the outlet of said main channel 1-8 communicates with sample outlet channel 1-10, and said sample outlet channel ...

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Abstract

The invention discloses a full-automatic ELISA detection device for treponema pallidum on a micro-fluidic chip. The full-automatic ELISA detection device comprises the micro-fluidic chip, a direct-current power source, a fluorescence detection system and a control system. The micro-fluidic chip comprises liquid storage tanks from first to seventh and a main channel, the liquid storage tanks from first to fifth are communicated with an inlet of the main channel through sample inlet channels, an outlet of the main channel is communicated with a sample outlet channel, and the other end of the sample outlet channel is communicated with the seventh liquid storage tank. The invention further discloses a detection method using the full-automatic ELISA detection device for treponema pallidum on the micro-fluidic chip. The full-automatic ELISA detection device has the advantages of being high in mechanization degree, simple in operation procedure, small in size, precise in structure, high in reliability and low in cost, and automatic control is convenient to achieve.

Description

technical field [0001] The invention relates to a device for automatic detection and separation of Treponema pallidum (Treponemapallidum, TP) and a detection method thereof. The present invention mainly realizes the automatic detection of Treponema pallidum in blood by ELISA on a microfluidic chip, specifically It is a fully automatic ELISA detection device and detection method for Treponema pallidum on a microfluidic chip. Background technique [0002] Treponema pallidum (TP) looks like a dense spring, with regular helix bending, straight ends, transparent, and not easy to color, so it is also called Treponema pallidum. Its infection in the human body can cause a chronic infectious disease - syphilis. Syphilis is highly contagious and harmful. After infection, it can cause damage to various tissues and organs in the whole body. In recent years, its incidence has been on the rise. Therefore, it is very important to choose a simple and accurate method for the detection of Tre...

Claims

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Application Information

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IPC IPC(8): G01N35/00
Inventor 张晓杰董海影李梦琪李冬青
Owner QIQIHAR MEDICAL UNIVERSITY
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