Nematode-trapping fungus powder preparation and application thereof
A powdery and fungal technology, applied in the field of microbiology, can solve the unsolved problems of batch production, standardization and effective content standardization of biocontrol agents, in the laboratory test stage, and difficult large-scale production, etc., to achieve the benefit of large-scale production Large-scale production, easy promotion and application, and the effect of avoiding waste
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Embodiment 1
[0017] (1) Preparation of nematode-predating fungus powder preparation
[0018] (1) Isolation, purification, isolation and identification of nematode-predating fungal isolates SDH035 and F088 From September 2013 to August 2014, samples from sheep manure piles in Wuzhong area of Ningxia and sheep identification samples in Yichun City, Heilongjiang Province, Separation using the improved plate spreading method. During separation, add the third stage larvae of sheep nematodes as bait to the plate and incubate at 25°C. Check under an inverted microscope once a day or every other day for 3 weeks to pick out a single predatory structure or prey Nematodes, transferred to 0.2% bran agar and cultured at 25°C for 3 to 14 days. After the conidia grew, pick a single conidia and put it on another 0.2% bran agar plate until a pure culture was obtained. . The morphology and data measurement of the conidia of the strain were obtained by the slide insertion method, and the DNA of the strain...
Embodiment 2
[0038] (1) Preparation of nematode-predating fungus powder preparation
[0039] (1) Isolation, purification, isolation and identification of nematode-predating fungal isolates SDH035 and F088 From September 2013 to August 2014, samples from sheep manure piles in Wuzhong area of Ningxia and sheep identification samples in Yichun City, Heilongjiang Province, Separation using the improved plate spreading method. During separation, the third-stage larvae of sheep nematodes are added to the plate as bait and hatched at 25°C. Check under an inverted microscope every day or every other day for 3 weeks to pick out a single predatory structure or prey Nematodes, transferred to 0.2% bran agar and cultured at 25°C for 3 to 14 days. After the conidia grew, pick a single conidia and put it on another 0.2% bran agar plate until a pure culture was obtained. . The morphology and data measurement of the conidia of the strain were obtained by the slide insertion method, and the DNA of the st...
Embodiment 3
[0057] (1) Preparation of nematode-predating fungus powder preparation
[0058] (1) Isolation, purification, isolation and identification of nematode-predating fungal isolates SDH035 and F088 From September 2013 to August 2014, samples from sheep manure piles in Wuzhong area of Ningxia and sheep identification samples in Yichun City, Heilongjiang Province, Separation using the improved plate spreading method. During separation, add the third stage larvae of sheep nematodes as bait to the plate and incubate at 25°C. Check under an inverted microscope once a day or every other day for 3 weeks to pick out a single predatory structure or prey Nematodes, transferred to 0.2% bran agar and cultured at 25°C for 3 to 14 days. After the conidia grew, pick a single conidia and put it on another 0.2% bran agar plate until a pure culture was obtained. . The morphology and data measurement of the conidia of the strain were obtained by the slide insertion method, and the DNA of the strain...
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