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Novel tissue-culture seedling raising method for aerospace breeding of Gentiana rigescens Franch

A space breeding and new method technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of ineffective preservation and propagation of gentian gentian space species, low seed maturity, long breeding cycle, etc. Achieve the effects of high survival rate of domestication and transplanting, simple components and low cost

Inactive Publication Date: 2020-05-19
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the yield of gentian gentian seeds is large, the seeds of gentian gentian are small (thousand-grain weight is only about 20mg), and the seed maturity is very low, which makes the germination rate of the seeds extremely low, while the seed setting and germination rate of gentian gentian aerospace breeding seeds are lower, making the Gentiana yunnanensis space species cannot be effectively preserved and multiplied. On the other hand, because gentiana yunnanensis belongs to heterofloral hybridization, the traits of the offspring and the female parent will be quite different after heterohybridization, and stable traits cannot be formed. Seed propagation cannot be the best way to reproduce the space species of Gentiana gentiana, and cutting propagation and branch propagation also have the disadvantages of extremely low reproduction rate and long reproduction cycle
Therefore, it is impossible to obtain space species of Gentiana yunnanensis with stable traits and sufficient quantity in a short period of time using traditional techniques.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: In the Yunnan gentian aerospace breeding and breeding base, the variety MB12 bred by the Yunnan gentian aerospace breeding was sprayed with 50% carbendazim 1000 times liquid and bagged to reduce the amount of bacteria. , When collecting explants, it will be soaked in 75% alcohol for more than 10s, and the branches within 10cm from the end will be cut;

[0020] Remove the leaves of the branches in the laboratory and cut them into 1~2cm budding stems, wash them in saturated soapy water for 10 minutes, and rinse them with tap water for 3~4 times until there is no foam on the surface, and then rinse with small running water for 1 hour;

[0021] Transfer the cleaned stems to a sterile bottle in an ultra-clean workbench, add 75% alcohol and shake for 20 seconds, rinse with sterile water for 3 to 4 times, add saturated bleach solution and shake for 30 minutes, use sterile Rinse with sterile water for 3-4 times, transfer to another sterile bottle, add 0.05% mercury l...

Embodiment 2

[0026] At the Yunnan Gentian Aerospace Breeding Base, the variety Z~1~2 selected by the Yunnan Gentian Aerospace Breeding was sprayed with 50% carbendazim 1000 times liquid and bagged to reduce the amount of bacteria. When explanting, it will be soaked in 75% alcohol for more than 10s, and the branches within 20cm from the end will be cut;

[0027] Remove the leaves of the branches in the laboratory and cut them into 1~2cm budding stems, wash them in saturated soapy water for 10 minutes, and rinse them with tap water for 3~4 times until there is no foam on the surface, and then rinse with small running water for 1 hour;

[0028] Transfer the cleaned stems to a sterile bottle in an ultra-clean workbench, add 75% alcohol and shake for 15 seconds, rinse with sterile water for 3 to 4 times, add saturated bleach solution and shake for 32 minutes, use sterile Rinse with sterile water for 3 to 4 times, transfer to another sterile bottle, add 0.05% mercury liter solution and shake for...

Embodiment 3

[0032] Embodiment 3: In the Yunnan gentian aerospace breeding base, the variety MB24 selected by the Yunnan gentian aerospace breeding was sprayed with 50% carbendazim 1000 times liquid to spray healthy plants without diseases and insect pests one month in advance and bagged to reduce the amount of bacteria. When collecting explants, it will be soaked in 75% alcohol for more than 10 seconds, and the branches within 20cm from the end will be cut;

[0033] Remove the leaves of the branches in the laboratory and cut them into 1~2cm budding stems, wash them in saturated soapy water for 10 minutes, and rinse them with tap water for 3~4 times until there is no foam on the surface, and then rinse with small running water for 1 hour;

[0034] Transfer the cleaned stems to a sterile bottle in an ultra-clean workbench, add 75% alcohol and shake for 20 seconds, rinse with sterile water for 3 to 4 times, add saturated bleach solution and shake for 30 minutes, use sterile Rinse with steril...

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Abstract

The invention provides a novel tissue-culture seedling raising method for Gentiana rigescens Franch on the basis of the characteristics of low germination rate and low cutting propagation rate of aerospace breeding seeds of the Gentiana rigescens Franch. The novel tissue-culture seedling raising method mainly comprises the following steps: selection and disinfection of explants, induction of cluster buds, proliferation of the cluster buds and culture of rooted seedlings. The method provided by the invention is simple and practicable, has the advantages of short propagation period, high propagation rate, capability of effectively stabilizing the characters of a mother plant and the like, and is the most effective way for producing Gentiana rigescens Franch aerospace species with stable characters on a large scale.

Description

technical field [0001] The invention relates to the field of biotechnology of flowers and Chinese medicinal materials, in particular to a tissue culture method for space breeding of Gentiana yunnanensis. [0002] technical background [0003] Gentian arigescens Franch. belongs to Gentianaceae Gentiana plant. It is one of the plant sources of the medicinal material Gentiana included in the Pharmacopoeia of the People's Republic of China, and it is also the main source of Gentiana medicinal material in Southwest China. Dian gentian is used as medicine with roots, mainly produced in Yunnan, Sichuan and other places. It has the effect of clearing away heat and dampness, and purging liver and gallbladder fire. Hypochondriac pain, bitter taste, strong middle, convulsion convulsion embolism. Modern studies believe that its main active ingredient is gentiopicroside, which has anti-inflammatory, analgesic, hypoxia-resistant, and anti-fatigue effects. [0004] In June 2013, Gentiana ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 杨绍兵张金渝左应梅杨美权杨天梅简邦丽
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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