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Rootless tissue culture method of lycoris radiata and lycoris radiata cultivating method

A cultivation method and technology of Lycoris, applied in the field of tissue culture of medicinal plants, can solve the problems of seedling damage, reduced seedling survival rate, wastage, etc.

Active Publication Date: 2020-05-08
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But this seedling hardening method needs to utilize rooting medium to carry out rooting, and after rooting is finished, seedling is taken out from medium and planted into hardening medium, needs to clean the medium that seedling root dips in before planting into medium, and in cleaning process It will cause certain damage and loss to the seedlings, which will reduce the survival rate of the seedlings
[0005] Therefore, it is urgent to provide a rootless tissue culture method for Lycoris with high survival rate, thereby overcoming the above-mentioned defects in the prior art

Method used

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  • Rootless tissue culture method of lycoris radiata and lycoris radiata cultivating method
  • Rootless tissue culture method of lycoris radiata and lycoris radiata cultivating method
  • Rootless tissue culture method of lycoris radiata and lycoris radiata cultivating method

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Embodiment 1-1

[0047] (1) Selection and disinfection of explants: The subterranean bulbs of Amaryllis lycoris, which grow vigorously and have basically the same size, are selected as explants at the stage of withered leaves for primary culture. The brown outermost layer of scales is peeled off, retained when the size of the bulb is stripped to 2cm, rinsed under running water for 60 minutes and then placed on an ultra-clean workbench. Cut off the root and 2 / 3 of the upper part of the bulb, and 1 / 3 of the remaining bulb for subsequent disinfection; wash the bulb twice with 200mg / L cephalosporin water, then wash it with 75wt.% alcohol for 30s, and then wash it with 0.5 Wash with wt.% benzalkonium bromide for 30 minutes, wash with sterile water for 5 times, and keep shaking the bulbs during the washing. Cut into 4 pieces according to the size of the bulb disk, each piece has 6 layers of scales and retains the base disk as explants.

[0048] (2) Induction of adventitious buds: the explants after...

Embodiment 1-2

[0051] Put the rootless tissue cultured seedlings obtained in Example 1-1 into the greenhouse, unscrew the bottle mouth of the tissue cultured seedlings in the greenhouse, inject a small amount of water into the bottle after 3 days, and take out the seedlings after hardening for 3 days. Gently take out the rootless test-tube seedlings of Lycoris after seedling hardening with tweezers. The rootless seedlings are soaked in a solution containing 0.2 mg / L IBA and 1 / 10,000 potassium permanganate for 5 minutes, rinsed with clean water, transplanted to a substrate for cultivation, and rooted Lycoris seedlings are obtained.

[0052] The transplanting medium contains peat: vermiculite: perlite, mixed according to the volume ratio of 12:10:1 and placed in a 10cm high square plate; after the square plate substrate is watered, the test tube seedlings are planted into the seedbed according to the row spacing of 5×6cm , when the test-tube seedlings are transplanted, all the small bulbs are ...

Embodiment 2-1

[0054] The proliferation culture cycle of embodiment 2-1 is 2 times, and all the other treatments are the same as embodiment 1-1.

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Abstract

The invention belongs to the field of tissue culture, and relates to a rootless tissue culture method of lycoris radiata and a lycoris radiata cultivating method. The rootless tissue culture method oflycoris radiata, provided by the invention comprises the steps of performing induction on bulb blocks in induction culture mediums so as to obtain adventitious buds, wherein the induction culture mediums use MS as a base, also comprise 0.5-3.0mg / L of N-(phenylmethyl)-9H-purin-6-amine, 0.5-1.5mg / L of alpha-naphthaleneacetic acid, 30g / L of cane sugar and / or white sugar and 6-8g / L of agar; and culturing the adventitious buds in propagation culture mediums so as to obtain rootless tissue culture seedlings, wherein the propagation culture mediums use MS as a base and also comprise 3.0-5.0mg / L of N-(phenylmethyl)-9H-purin-6-amine, 0.5-1.5mg / L of alpha-naphthaleneacetic acid, 0-3mg / L of forchlorfenuron, 30g / L of cane sugar and / or white sugar, 0-1mg / L of thidiazuron and 7-8g / L of agar, and numberof times of propagation culture is 1-5. Through the adoption of the rootless tissue culture method of lycoris radiata provided by the invention, the survival rate is high.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of medicinal plants, and in particular relates to a rootless tissue culture method of Lycoris and a cultivation method of Lycoris. Background technique [0002] Lycoris radiata (Lycoris radiata) is an important medicinal and ornamental plant in my country. It has bright colors and strong ornamental value, and its bulbs contain various alkaloids such as lycoris radiata and galantamine. Dementia (Alzheimer's disease, AD), anti-tumor and anti-cancer and other important functions, the economic value is extremely high. Due to the low natural reproduction coefficient of lycoris, it is not easy to grow normally, and only 1 to 2 seed bulbs can be produced each year by dividing the bulbs, and it takes 3 to 5 years after planting to form a flowering bulb, and the growth cycle is long. At present, the reproduction period cannot be greatly shortened. Therefore, greatly increasing the reproduction coeff...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
CPCA01H4/005A01G31/00
Inventor 李青竹蔡友铭张永春殷丽青许俊旭周晓慧
Owner SHANGHAI ACAD OF AGRI SCI
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