39 results about "Rhodiola sachalinensis" patented technology

The invention relates to a separating purification method of a natural rhodioloside, which particularly belongs to the technical field of plant extraction deep-processing and is characterized by comprising the steps that: natural rhodiola sachalinensis is taken as a material which is crashed, subjected to alcoholic solvent extraction, two-step macroporous resin absorption and desorption, absolute ethyl alcohol crystallization, crystal mush centrifugalization and absolute ethyl alcohol washing, and later dried in vacuum so as to obtain high purity natural rhodioloside product. The purity of the rhodioloside extracted by the method can be up to over 99 percent, the total recovery coefficient of the rhodioloside can be up to over 40 percent in the whole process; the method has simple and convenient separating purifying technique and large treatment amount, is applicable to industrializing production, can provide technical support for deep development and utilization of the rhodiola sachalinensis resources, and provide excellent natural materials for medicine and function food industrialization.

The present invention discloses a method using Agrobacterium rhizogenes (Ar) gene to transform Rhodiola sachalinensis A.Bor to achieve a hairy root system for Salidroside production. By preparation of explant and cultivation in Ar bacteria liquid, Rhodiola sachalinensis A.Bor is induced to grow hairy roots by genetic transformation; the PCR testing proves that hairy roots are generated by transformation; a fermenter is used for massive production of hairy roots, and precursor substances and elicitors are added to improve the Salidroside content. The method is applied to produce Salidroside to substitute the drying-up Rhodiola sachalinensis A.Bor resources.

The invention discloses a rhodiola rosea extract product, a preparation method and an application. The preparation method of the rhodiola rosea extract product comprises the following steps: extracting the root of rhodiola rosea by using 95% of ethanol aqueous solution, and collecting an extract and removing ethanol in the extract to obtain 95% of ethanol extract product of rhodiola rosea, wherein the 95% of ethanol extract product of rhodiola rosea is the rhodiola rosea extract product. The experiment proves that when the concentration of the 95% of ethanol extract product of rhodiola crenulata, 95% of ethanol extract product of rhodiola arctica and 95% of ethanol extract product of rhodiola sachalinensis is 1mg / ml, the rhodiola rosea extract product has obvious inhibition effect for tomato Botrytis germs and cabbage black spot germs. The rhodiola crenulata, rhodiola arctica and / or rhodiola sachalinensis can be used for preparing an inhibitor for tomato Botrytis germs and cabbage black spot germs.

The invention discloses duck feed containing ramie root and a production method thereof. The duck feed comprises the following raw materials in parts by weight: maize, wheat bran, bean pulp, buckwheat, soybean protein powder, silkworm chrysalis, vinegar residue, euphorbia thymifolia, silybum marianum, cowherb seed, eclipta, rhodiola sachalinensis, salvia prionitis, gynostemma pentaphylla, the ramie root, sweet potato leaf, elaeagnus pungens, fried barley, salt, a premix compound, rose essence essential oil, ferrous sulfate, lysine, threonine, a probiotic preparation and choline. According to a formula of the duck feed, the buckwheat, the soybean protein powder, the silkworm chrysalis and the vinegar residue are high in nutrient content and can be used for improving the growth state of ducks and the quality of duck meat; the eclipta, the gynostemma pentaphylla and the elaeagnus pungens can be used for enabling the duck body to be strong, improving the resistance to diseases and reducing the occurrence rate of duck diseases; the euphorbia thymifolia and the ramie root have the effect of removing endotoxin and can be used for reducing the infection probability of duck diseases; and the whole formula is scientific and reasonable.

The invention belongs to the field of rhodiola sachalinensis cultivation and provides a method for cultivation of an improved variety of rhodiola sachalinensis in a low-altitude plain area. The method aims to solve the technical problems that in the process of cultivation of the rhodiola sachalinensis, root rot is prone to occurrence, the rate of emergence of seeds is low, and the yield is low. According to the method for cultivation of the improved variety of the rhodiola sachalinensis in the low-altitude plain area, a production cycle lasts for four years, a sowing plate is used for sowing, a bed surface is covered with pine needles and a mulching film, a shading net is erected on a bed, and weeding is conducted in time; in the next year, field planting is conducted, and organic compound fertilizer and farmyard manure which is processed through ferment bacteria are applied additionally; in the third year, after the seeds are harvested, stems and leaves above the ground are cut off, and field cultivation, vegetative propagation and other measures are taken; in the fourth year, fertilization is conducted from early May, and other aspects are managed according to the third year. By means of the method, the rate of emergence is over 90.5% which is more than three times that of common seeds which are sown, the morbidity of root rot is below 0.01%, the purity of the seeds is up to 100%, and the content of salidroside is up to 0.88%.

The invention relates to a separation and purification method of salidroside in natural rhodiola sachalinensis. The separation and purification method is characterized by comprising the steps of: slicing the natural rhodiola sachalinensis serving as a raw material; carrying out ultrasonic water leaching, low-temperature alcohol participation and vacuum concentration to obtain a water extract; carrying out solvent stripping and impurity removal, solvent extracting and back-extracting to obtain a high-purity salidroside water solution; and finally, carrying out vacuum concentration, absolute ethyl alcohol crystallization, separation and drying to obtain the salidroside. The purity of the salidroside extracted by adopting the separation and purification method can reach above 97 percent; the yield in a purification stage of a novel process is greatly increased, the yield in an extraction and purification stage of salidroside can reach above 70 percent, and the raw material utilization rate is increased; an addition product tyrosol enriching solution can be obtained; and the separation and purification method is simple in process, convenient for operation and suitable for industrialized massive production.

The invention discloses an application and use method of salidroside in preparing a skin-care product for preventing skin photoaging. When the skin-care product is prepared, the salidroside can be taken as an active matter and prepared into a salidroside liposome with the grain diameter less than 200 nanometers, and the mass percent concentration of the salidroside liposome is 0.01%-10%. According to the application and the use method disclosed by the invention, the influence of the salidroside on the expression of a UVB (Ultraviolet B) induced skin keratinocyte photoaging key gene is inspected, a test result indicates that the skin-care product effectively inhibits the degradation of collagen and the inflammatory reaction of cells and achieves the effect of preventing the skin photoaging; the salidroside is one of main components of rhodiola sachalinensis, and the salidroside can be directly added to the skin-care product for preventing the skin photoaging, or a salidroside extract which contains the salidroside can be indirectly added to the skin-care product for preventing the skin photoaging.

The invention relates to a traditional Chinese medicine composite with anti-tumor effect and a preparation method of an injection of the same. The traditional Chinese medicine composite comprises the following components in percentage by weight: 7.56%-43.48% of radix semiaquilegiae, 12.00%-47.62% of solanum nigrum, 12.00%-47.62% of radix ranunculi ternati, 5.17%-42.67% of hairyvein agrimony and 2.44%-30.77% of rhodiola sachalinensis, wherein the sum of the weight percentage of the components is 100%. The preparation method comprises the following steps of: weighing materials; extracting, and refining; embedding; sterilizing. The traditional Chinese medicine composite disclosed by the invention keeps the characteristic of the integrated action of multiple component, multiple target point and multiple effects of Chinese herbal medicines, inhibits the tumor cell and prevents the diffusion transfer of the tumor cell through multiple action mechanisms, can not generate the drug resistance in a using process, can improve the symptoms and the physical signs, enhance the survival quality and prolong the survival time and is a Chinese herbal medicine anti-tumor formulation with wide application prospect.

The invention discloses a preparation method of high-purity salidroside, which comprises the following steps: crushing rhodiola sachalinensis as a raw material, and then adding a 95% ethanol solution for soaking and percolating; sucking supernatant liquid after the percolate is in a standing state, and adsorbing by using weak-polar macroporous resin to remove polysaccharide and protein; sampling, then washing with purified water until the supernatant liquid is colorless, and carrying out gradient elution; separating a salidroside crude product in a RP-HPLC (Reversed-Phase High Performance Liquid Chromatography), and carrying out gradient elution by taking a methanol aqueous solution as a mobile phase; when the target peak occurs, collecting elution solutions in sections; and freeze-drying the elution solution in which the purity of salidroside is higher than 98%, thereby obtaining high-purity salidroside. According to the invention, impurities including saccharide and protein are removed by percolation and macroporous resin adsorption to obtain the salidroside crude product; after the impurities including saccharide and protein are removed, the degree of purification of the RP-HPLC can be favorably improved; and through reasonable gradient elution and elution solutions, the purity and yield of the product are further increased.

The invention discloses a pleurotus nebrodensis / lycopodium clavatum composite beverage which is characterized by being prepared from the following raw materials in parts by weight: 25-30 parts of tremella, 20-25 parts of pleurotus nebrodensis, 15-18 parts of hericium erinaceus, 1-2 parts of rhodiola sachalinensis, 1-2 parts of lycopodium clavatum, 1-2 parts of red ginseng, 2-4 parts of grifola, 3-5 parts of oriental wormwood, 1-2 parts of rhizoma corydalis, 12-15 parts of raspberry, 14-17 parts of lycium chinense, 10-13 parts of cinnamon, 13-15 parts of soybean, 0.3-0.5 part of lutein, 14-18 parts of strawberry, 15-18 parts of perilla leaf, 17-19 parts of gizzards, 5-6 parts of additive and a proper amount of water. The beverage disclosed by the invention can cool, reduce the blood press and enhance the human body immunity and disease resistance, can be used as a series healthcare products for auxiliary treatment on hepatitis B; the fungi such as hericium erinaceus and pleurotus nebrodensis are rich in nutrition, so that the beverage has the effects of promoting longevity, nourishing and strengthening, removing heat from the liver, strengthening tendons and bones, preventing fatigue and preventing aging after being taken for a long time.

The invention relates to the technical field of cosmetics, and discloses a soothing body milk mainly composed of root extract of Rhodiola sachalinensis. The active ingredient comprises rhodiola root extract, sodium dehydroacetate, rhizoma polygonati extract, phenoxyethanol and ethylhexyl glycerol. The soothing body milk of the invention has no irritation to skin, can effectively keep skin moisture, delay skin aging, restore skin elasticity, and has simple preparation process.

The present invention discloses a method using Agrobacterium rhizogenes (Ar) gene to transform Rhodiola sachalinensis A.Bor to achieve a hairy root system for Salidroside production. By preparation of explant and cultivation in Ar bacteria liquid, Rhodiola sachalinensis A.Bor is induced to grow hairy roots by genetic transformation; the PCR testing proves that hairy roots are generated by transformation; a fermenter is used for massive production of hairy roots, and precursor substances and elicitors are added to improve the Salidroside content. The method is applied to produce Salidroside to substitute the drying-up Rhodiola sachalinensis A.Bor resources.

The invention relates to a Chinese materia medica preparation, which is a decoction medicine for treating chronic bronchitis emptysis. The medicine is prepared by the raw materials according to the parts by weight as follows: 13-15g of rhodiola sachalinensis, 13-15g of gynura bicolor, 8-10g of kweichow sage herb and 8-10g of fructus aristolochiae. The decoction medicine has the characteristics of low cost, easy preparation, insignificant toxicity and side effects and remarkable healing efficacy.

The invention discloses a body-building health-care product. The body-building health-care product is prepared from the following natural medicines in parts by weight: 10-20 parts of Rhodiola sachalinensis, 5-10 parts of cistanche, 30-50 parts of the fruit of Chinese wolfberry, 5-10 parts of pearl powder, 30-60 parts of radix curcumae longae, 20-40 parts of rhizoma polygonati, 10-15 parts of glossy privet fruit, and 5-10 parts of Angelica sinensis. The body-building health-care product adopting a plurality of kinds of natural medicines is harmless to a human body, and has the effects of regulating metabolism of the human body, invigorating the blood circulation and building the body, improving the immunity of the human body, and enabling people to resist aging, have health body and prolong the life.

A pharmaceutical composition for treating and preventing cancer, and health food for ameliorating and preventing cancer containing the pharmaceutical composition are provided. The pharmaceutical composition includes rhodiola sachalinensis, oldenlandia diffuse, and cistanchis herba to increase the expression of a cancer suppressor gene and thus treat and prevent cancer by suppressing the growth of cancer cells while not affecting normal cells.

A moose brain Rhodiola anoxia resistant heath care capsule belongs to the field of health care food and is prepared by taking moose brain, rose hip tea, Rhodiola sachalinensis, poria cocos and chrysanthemum as raw materials through the steps of drying, crushing, alcohol extracting, water extracting and other processes. The health care capsule can obviously alleviate discomfort and impairment on human body by anoxia, has good health care effect and no toxic or side effect, and is suitable for people in anoxic environments to take.

The invention discloses a feed for ewes in suckling period, and a preparation method thereof, and belongs to the field of feed. The feed is prepared from, by weight, 30 to 40 parts of corn flour, 4 to 10 parts of pumpkin powder, 6 to 12 parts of zeolite powder, 4 to 10 parts of bone meal, 4 to 8 parts of cottonseed meal, 6 to 12 parts of fish meal, 5 to 15 parts of soya bean meal, 4 to 10 parts of shell powder, 1 to 2 parts of amino acid, 1 to 2 parts of a vitamin additive, 1 to 2 parts of table salt, 4 to 10 parts of cortex fraxini, 6 to 12 parts of coriander seed, 4 to 10 parts of spica prunellae, 6 to 12 parts of rhizoma polygoni cuspidati, 4 to 10 parts of rhodiola sachalinensis, 4 to 10 parts of folium mori, 6 to 12 parts of liquorice, 4 to 10 parts of semen alpiniae katsumadai, and 6 to 12 parts of pericarpium citri reticulatae. The feed is scientific in formula, is abundant in nutrients, and is capable of satisfying requirements of growth and physiological characteristics of ewes in suckling period, providing ewes in suckling period with nutrients needed by growth, promoting digestion and absorption, and improving immunity of ewes at the same time.

The invention discloses a pigskin hawthorn sour ginger paste, which is prepared from the following raw materials in parts by weight: 300-310 parts by weight of ginger, 56-58 parts of hawthorn fruit, 21-23 parts of pigskin, 15-17 parts of chrysanthemum vinegar, 3-4 parts of star anise and shredded jellyfish 40-45, pomegranate seed powder 13-15, reed root 3-3.2, cassava seed 2.4-3, dogwood 2.3-2.5, rhodiola 3.3-3.5, malic acid 1.2-1.4, brown sugar 90-95 and water. The ginger juice extracted during the processing of the ginger paste of the present invention has a good effect, and the ginger juice is combined with various auxiliary materials, such as hawthorn fruit, pig skin, pomegranate seed powder, etc., so that the processed ginger paste has many nutritional components and comprehensive nutrition. It also overcomes the shortcomings of single taste and poor flavor, has high nutritional value, and is easy to be digested and absorbed by the human body.

The invention discloses a functional health care flour taking affinal drug and diet Chinese herbal medicine including trichosanthes kirilowii maxim, rhodiola sachalinensis, fructus toosendan, sweetening chrysanthemum, polygonum multiflorum, angelica sinensis, fruit of immature citrons, caulis spatholobi, magnolia officinalis, liquorice and the like as raw materials. The flour product has the functions of relaxing bowels, tonifying spleen and lungs, reinforcing the stomach and tonifying kidney and preventing and curing constipation, and the multilevel requirements of pursuing taste, nutrition and heath care by people can be met. The flour powder has the advantages that the Chinese herbal medicine functional ingredients extracted by the technology of 'secondary ultrasonic-assisted digestion+ boiling' are added with enzymic preparations such as cellulose, amylase, saccharifying enzyme and xylanase, not only is the leaching efficiency of Chinese herbal medicine active ingredients improved, but also egg white, xylan and the like are degraded into water-soluble functional activity ingredients such as polypeptide and xylooligosaccharide, and therefore the nutritional performance and the functionality of the enriched flour are improved; through the scientific compatibility of the various types of Chinese herbal medicine, the synergistic effect between the Chinese herbal medicine raw materials is fully played, and the constipation prevention function of the enriched flour is improved.

The invention discloses a traditional Chinese medicine composition for treating essential hypotension, which is prepared from the following raw medicines: deerhorn glue, oxhide glue, eclipta, mulberry, cassia, spiny jujube, flowery knotweed stem, puerariae, acanthopanax, rhodiola sachalinensis and fresh ginger. The traditional Chinese medicine composition for treating essential hypotension provided by the invention fills the blank of no special medicines on treating essential hypotension, and is obvious in treatment effect and convenient to use. After 73 patients are treated for 2 weeks by adopting the traditional Chinese medicine composition water decoction provided by the invention, 29 patients are cured, 41 patients take a turn for the better, 3 patients do not take effect, and total effective rate is 95.89%.

The invention discloses a fast culture method of rhodiola kirilowii(regel)maxim. seedlings; the method takes rhodiola kirilowii(regel)maxim. cotyledonary petioles as explants, through induction culture of explant embryogenic cells and embryoids, the rhodiola kirilowii(regel)maxim. high-quality seedlings are successfully cultured, and a new way is provided for massive fast culture of the rhodiola kirilowii(regel)maxim. high-quality seedlings.

The invention discloses a rhodiola rosea extract product, a preparation method and an application. The preparation method of the rhodiola rosea extract product comprises the following steps: extracting the root of rhodiola rosea by using 95% of ethanol aqueous solution, and collecting an extract and removing ethanol in the extract to obtain 95% of ethanol extract product of rhodiola rosea, wherein the 95% of ethanol extract product of rhodiola rosea is the rhodiola rosea extract product. The experiment proves that when the concentration of the 95% of ethanol extract product of rhodiola crenulata, 95% of ethanol extract product of rhodiola arctica and 95% of ethanol extract product of rhodiola sachalinensis is 1mg / ml, the rhodiola rosea extract product has obvious inhibition effect for tomato Botrytis germs and cabbage black spot germs. The rhodiola crenulata, rhodiola arctica and / or rhodiola sachalinensis can be used for preparing an inhibitor for tomato Botrytis germs and cabbage black spot germs.

The invention discloses a rhodiola sachalinensis water extract prepared by the steps: drying roots of rhodiola sachalinensis, adding water for soaking, filtering, collecting filtrate and filter residues, adding water into the filter residues for decocting, filtering after decocting, collecting filtrate, mixing the two filtrates, concentrating, and freeze-drying to obtain the rhodiola sachalinensisaqueous extract; and carrying out macroporous resin dynamic adsorption-elution, eluting with water, discarding the eluent, eluting with a 10% ethanol solution, collecting the eluent, evaporating to remove the solvent, and thus obtaining the rhodiola crenulata extract. When the concentration of the rhodiola sachalinensis aqueous extract is 2 mg / mL, the inhibition rate of the rhodiola sachalinensisaqueous extract on ginseng rhizoctonia solani kuhn can reach 100%, and when the concentration of the rhodiola sachalinensis aqueous extract is 3 mg / mL, the inhibition rate of the rhodiola sachalinensis aqueous extract on ginsengrhizoctonia solani kuhn is 50%. When the refined rhodiola sachalinensis extract is 0.5 mg / mL, the inhibition rate of the rhodiola sachalinensis extract on ginseng rhizoctonia solani kuhn is 47.47%, and the inhibition rate of the rhodiola sachalinensis extract on ginseng rhizoctonia solani kuhn reaches 97.47%.

The invention discloses fusion protein, as well as an efficient expression method and application thereof and belongs to the technical field of fusion protein. The fusion protein is AtSUS1-RsUGT72B14fusion protein, including core fragments of arabidopsis thaliana sucrosesynthaseAtSUS1 and rhodiola sachalinensis uridine diphosphate glucosyl transferase RsUGT72B14. According to the codon preferenceof escherichia coli, an optimized gene sequence of the AtSUS1-RsUGT72B14 fusion protein is obtained by combining the characteristics of gene sequences of AtSUS1 and RsUGT72B14 and introducing GGSG four amino acid connecting peptide sequences by designing a restriction enzyme cutting site connection method, efficient expression of the AtSUS1-RsUGT72B14 fusion protein is realized, and the fusion protein can be used for catalytically synthesizing salidroside.

The invention relates to a culture method of an aseptic Rhodiola sachalinensis A.Bor. seedling having developed roots and belongs to the field of modern biological methods. The culture method comprises the following steps of 1, acquiring a wild Rhodiola sachalinensis A.Bor. seedling, 2, carrying out disinfection treatment on stem of the acquired wild Rhodiola sachalinensis A.Bor. seedling, 3, carrying out cutting treatment on the young stem of Rhodiola sachalinensis A.Bor. seedling, 4, preparing a medium, and 5, carrying out culture of the aseptic Rhodiola sachalinensis A.Bor. seedling having developed roots. The culture method solves the problem that in the existing Rhodiola sachalinensis A.Bor. tissue culture, Rhodiola sachalinensis A.Bor. seedling growth is weak, root production is difficult, the number of roots is less and seedling age is short. Through the culture method of the aseptic Rhodiola sachalinensis A.Bor. seedling having developed roots, a high-quality Rhodiola sachalinensis A.Bor. regenerated plant is obtained.

The invention discloses a cultivation method for improving the biomass of rhodiola kirilowii and belongs to the technical field of rhodiola cultivation. The cultivation method comprises the following steps of (1) moisture treatment, (2) light treatment and (3) fertilization treatment. According to the cultivation method for improving the biomass of the rhodiola kirilowii, the biomass of the rhodiola kirilowii can be effectively improved; the biomass of the rhodiola kirilowii is improved by 38.39% under moisture treatment; the biomass of the rhodiola kirilowii is improved by 23.75% under light treatment; and the biomasses of the rhodiola kirilowii to which nitrogen fertilizers, phosphorus fertilizers and potassium fertilizers are applied are improved by 159.76%, 136.59% and 248.78% correspondingly.

The invention discloses duck feed containing bamboo charcoal and a production method thereof. The duck feed comprises the raw materials in parts by weight: maize, wheat bran, rice bran, fresh rhizoma phragmitis, garlic bolt, maize powder, silkworm chrysalis, vinegar residue, brewer's grain, uncaria rhynchophylla, rhodiola sachalinensis, salvia prionitis, eclipta, costus root, Chinese alpine rush, Chinese knotweed head, the bamboo charcoal, sweet potato leaf, beautyberry leaf, elaeagnus pungens, salt, a premix compound, organic selenium, calcium hydrogen phosphate, lysine, threonine, organic chromium and choline. According to a formula of the duck feed, the fresh rhizoma phragmitis, the maize powder, the silkworm chrysalis, the vinegar residue and the brewer's grain are high in nutrient content and can be used for improving the growth state of ducks and the quality of duck meat; the uncaria rhynchophylla and the Chinese alpine rush can be used for enabling the duck body to be strong, improving the resistance to diseases and reducing the occurrence rate of duck diseases; and the Chinese knotweed head has the effect of removing endotoxin; the bamboo charcoal has the effect of adsorbing the endotoxin; the garlic bolt has the bactericidal effect and can be used for reducing the infection probability of duck diseases, so that the whole formula is scientific and reasonable.

The invention belongs to the technical field of health care food processing and relates to a new preparation of a Ficus carica Linn health care product which is prepared by the raw materials of Ficus carica Linn, Dioscorea opposita Thunb, Rhodiola sachalinensis A.Bor., Glycine max(L.)Merr., Hippophae rhamnoides L., pine pollen (the pure and dry pollen of Pinus Massoniana Lamb. and Pinus tabulaeformis Carr.), Ziziphus jujuba Mill.var.inermis(Bunge)Rehd., maltitol and the like. Glycine max(L.)Merr., Rhodiola sachalinensis A.Bor. and Ziziphus jujuba Mill.var.inermis(Bunge)Rehd are boiled in water for extracting biological active ingredients, Ficus carica Linn and Hippophae rhamnoides L. are subject to juice squeezing, Glycine max(L.)Merr. is soaked and prepared into cooked soybean milk whichis spray-dried to obtain bean powder, and the wall of pine pollen is broken by being ground on a high-pressure micro-powder pulverizing mill. The preparation can keep the content of relevant biological active ingredients of Ficus carica Linn and other raw materials, is suitable for industrial production, has low cost and reliable quality, is applicable to various groups, is suitable for being taken with water at breakfast and can achieve the function of health care to the human body. Moreover, by adopting the invention, the varieties of the products prepared by Ficus carica Linn are increasedand the problems of storing and transporting the fresh fruits of Ficus carica Linn are solved.

The invention discloses a composition for enhancing immunity and resisting oxidization, a preparation and a preparation method thereof. The composition comprises the following components in parts by weight: 100-150 parts of sheep placenta freeze-dried powder, 50-90 parts of rhodiola sachalinensis extract and 50-90 parts of grape seed extract. The composition is capable of regulating the immune function of a body and effectively eliminating excessive free radicals in the body to maintain balance between the free radicals in the body and antioxidant enzymes, thereby playing roles in enhancing immunity and resisting oxidization. The preparation is stable in property, good in absorption and high in bioavailability and increases the drug effect.