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Isolation and purification method of natural salidroside

A technology for the separation and purification of salidroside, which is applied in the field of separation and purification of natural salidroside, can solve the problems of wasting organic solvents, organic solvent residues, and low separation efficiency, and achieves large processing capacity, good regeneration performance, and process easy effect

Active Publication Date: 2009-03-25
上海国矗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method uses multi-step solvent extraction combined with silica gel column and polyamide column chromatography, which has the disadvantages of wasting organic solvent, high energy consumption, low separation efficiency, residual organic solvent and high labor intensity
Although high-speed countercurrent chromatography (HSCCC) can be used to prepare high-purity salidroside, this method also has the disadvantages of high cost, residual organic solvents and unsuitability for industrialization.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The separation and purification method of a kind of natural salidroside of the present invention adopts the following process steps:

[0020] 1. Extraction: crush the Rhodiola rosea raw material into Rhodiola rosea powder material, the particle size of Rhodiola rosea powder material is 0.1-5mm, extract the crushed Rhodiola rosea powder material with a certain concentration of ethanol, the extraction condition is : the mass percent concentration of ethanol aqueous solution is 70%, the ratio of rhodiola rosea powdery raw material and ethanol aqueous solution is 1:6, the extraction temperature is 60 ℃, extract twice, each extraction time 1h, obtain rhodiola rosea extract;

[0021] 2. The first step of separation and purification of macroporous resin: at normal temperature, concentrate the Rhodiola rosea extract until it has no alcohol smell, then add water to dilute it into a sample salidroside clarified solution with a concentration of 1.89 mg / ml (volume dilution ratio of ...

Embodiment 2

[0027] The separation and purification method of a kind of natural salidroside of the present invention adopts the following process steps:

[0028] 1. Extraction: use Rhodiola rosea as the raw material and crush it into rhodiola powder. The particle size of the rhodiola powder is 0.1-5 mm. The crushed rhodiola powder is extracted with a certain concentration of ethanol. The extraction conditions for:

[0029] The mass percent concentration of the ethanol aqueous solution is 0-95%, the ratio of Rhodiola rosea powdered raw material to the ethanol aqueous solution is 1:6-1:20, the extraction temperature is 25-100°C, and the extraction is performed twice, and the extraction time is 0.5-100°C each time. 2h, obtain Rhodiola rosea extract;

[0030] 2. The first step of separation and purification of macroporous resin: at room temperature, concentrate the Rhodiola rosea extract until it has no alcohol smell, then add water to dilute it into a sample salidroside clarified solution wi...

Embodiment 3

[0035] The separation and purification method of a kind of natural salidroside of the present invention adopts the following process steps:

[0036] 1. Extraction: use Rhodiola rosea as the raw material and crush it into rhodiola powder. The particle size of rhodiola powder is 0.1-0.8mm. The conditions are: the mass percent concentration of ethanol aqueous solution is 20%, the ratio of Rhodiola rosea powdered raw material to ethanol aqueous solution is 1:20, the extraction temperature is 75°C, and the extraction is performed twice, each extraction time is 1h, and the Rhodiola rosea extract is obtained. liquid;

[0037] 2. The first step of separation and purification of macroporous resin: at room temperature, concentrate the Rhodiola rosea extract until it has no alcohol smell, then add water to dilute it into a clarified salidroside solution with a concentration of 5 mg / ml, and apply Rhodiola rosea Glycoside clarified liquid is adsorbed by a chromatographic column equipped w...

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Abstract

The invention relates to a separating purification method of a natural rhodioloside, which particularly belongs to the technical field of plant extraction deep-processing and is characterized by comprising the steps that: natural rhodiola sachalinensis is taken as a material which is crashed, subjected to alcoholic solvent extraction, two-step macroporous resin absorption and desorption, absolute ethyl alcohol crystallization, crystal mush centrifugalization and absolute ethyl alcohol washing, and later dried in vacuum so as to obtain high purity natural rhodioloside product. The purity of the rhodioloside extracted by the method can be up to over 99 percent, the total recovery coefficient of the rhodioloside can be up to over 40 percent in the whole process; the method has simple and convenient separating purifying technique and large treatment amount, is applicable to industrializing production, can provide technical support for deep development and utilization of the rhodiola sachalinensis resources, and provide excellent natural materials for medicine and function food industrialization.

Description

technical field [0001] The invention relates to a method for separating and purifying natural salidroside, and specifically belongs to the technical field of plant extraction and deep processing. Background technique [0002] Salidroside (saliroside) is one of the main active ingredients of Rhodiola, which has anti-lipid peroxidation, free radical damage to rat cortical nerve cells, protective effect on cardiovascular system, and liver protection. And anti-hepatic fibrosis, anti-fatigue, anti-depression, anti-cancer and anti-aging effects. Salidroside has wide application value in pharmaceutical and health food industries. The existing methods for the preparation of high-purity salidroside are mainly some traditional methods, which lack the preparation technology of high-purity products suitable for industrialization. The traditional method uses multi-step solvent extraction combined with silica gel column and polyamide column chromatography, which has the disadvantages of...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08
Inventor 王洪新马朝阳殷梦龙杭晓敏
Owner 上海国矗生物科技有限公司
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