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Culture method of aseptic Rhodiola sachalinensis A.Bor. seedling having developed roots

A cultivation method, Rhodiola rosea technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of short seedling age, few roots, weak seedling growth, etc., to simplify the cultivation procedure and reduce the cost of experiments , conducive to the effect of accumulation

Inactive Publication Date: 2014-06-18
JILIN NORMAL UNIV
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  • Description
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  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to overcome the problems such as weak seedling growth potential, difficult rooting, fewer roots, and short seedling age in the existing Rhodiola alpine tissue culture process, and provide a method for cultivating Rhodiola aseptic seedlings with well-developed root system, To obtain high-quality regenerated plants of Rhodiola rosea

Method used

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  • Culture method of aseptic Rhodiola sachalinensis A.Bor. seedling having developed roots
  • Culture method of aseptic Rhodiola sachalinensis A.Bor. seedling having developed roots
  • Culture method of aseptic Rhodiola sachalinensis A.Bor. seedling having developed roots

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Embodiment Construction

[0020] Below in conjunction with implementation case the present invention will be further described:

[0021] (1) Collection of wild seedlings of Rhodiola rosea: In July every year, wild seedlings of Rhodiola rosea are collected at an altitude of 1700-2500 m in Changbai Mountain. When selecting seedlings, young stems should be selected, and the distance from the root should be 2cm. For the above parts, care should be taken to keep the tender stems fresh when selecting the young stems.

[0022] (2) Disinfect the collected stems of Rhodiola rosea wild seedlings: rinse them with running water for 10 minutes, soak them in 75% alcohol for 30 seconds in an ultra-clean workbench, rinse them twice with sterile water, and then use Soak in 10% NaClO solution for 10 min, rinse with sterile water 4 times, and set aside.

[0023] (3) Segmentation of the young stems of Rhodiola rosea: Put the sterilized young stems of Rhodiola rosea in a petri dish, put them on the ultra-clean workbench, ...

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Abstract

The invention relates to a culture method of an aseptic Rhodiola sachalinensis A.Bor. seedling having developed roots and belongs to the field of modern biological methods. The culture method comprises the following steps of 1, acquiring a wild Rhodiola sachalinensis A.Bor. seedling, 2, carrying out disinfection treatment on stem of the acquired wild Rhodiola sachalinensis A.Bor. seedling, 3, carrying out cutting treatment on the young stem of Rhodiola sachalinensis A.Bor. seedling, 4, preparing a medium, and 5, carrying out culture of the aseptic Rhodiola sachalinensis A.Bor. seedling having developed roots. The culture method solves the problem that in the existing Rhodiola sachalinensis A.Bor. tissue culture, Rhodiola sachalinensis A.Bor. seedling growth is weak, root production is difficult, the number of roots is less and seedling age is short. Through the culture method of the aseptic Rhodiola sachalinensis A.Bor. seedling having developed roots, a high-quality Rhodiola sachalinensis A.Bor. regenerated plant is obtained.

Description

technical field [0001] The invention relates to a method for cultivating Rhodiola rosea aseptic seedlings with well-developed root system, and belongs to the field of modern biological methods. Background technique [0002] Alpine Rhodiola (Rhodiola s activated carbon halinensis A. Bor.), also known as Sakye Rhodiola, belongs to the genus Rhodiola (Rhodiola L.) of the Sedum family. It is a perennial dicotyledonous herb that grows in high-altitude and low-temperature mountainous areas. . In my country, it is mostly distributed in the northeast, and it is one of the rare and precious medicinal plants in the Changbai Mountain area. The roots, stems, leaves, flowers, and seeds of Rhodiola alpine can be used as medicine. The active ingredients are salidroside, flavonoids and various trace elements. Rhodiola not only has anti-oxidation, anti-fatigue, anti-hypoxia, Anti-cold, anti-aging, anti-microwave radiation and other similar "adaptogen" effects. Due to the large demand of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 周晓馥徐洪伟未晓巍
Owner JILIN NORMAL UNIV
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