Experimental device for in-vitro simulated blood vessel microenvironment

Abstract

The invention provides an experimental device for a simulated blood vessel microenvironment. The device comprises a card type flow chamber, a peristaltic pump and a liquid storage tank, wherein the card type flow chamber as a core component of the device has a rectangular base and the center of the base is provided with a step which is provided with an O-shaped seal groove and a micro-reagent storage pool; a microporous membrane is covered on the storage pool; an O-shaped gasket ring is arranged on the microporous membrane; the membrane is flattened by a clamping plate with a rectangular groove and a central through hole, and is fixed on the step of the rectangular base; the base is matched with an upper cover which is provided with a T-shaped inflow port and an outflow port; and the rectangular groove of the clamping plate is closed to form a main flow field area; the clamping flow chamber, the peristaltic pump and the liquid storage tank are connected into a whole through pipelines; a lower liquid storage tank is connected with a vent hole which is provided with a filter membrane to keep constant system pressure. The device flattens and fixes the membrane by pushing of the clamping plate, and forms a micro-chemical cabin under the membrane, and the main flow field area is a flaky laminar flow, thereby providing the novel experimental device for the in-vitro simulated blood vessel microenvironment used in micro-chemical experiments with high flow field stability and low experiment cost.

Description

1. Technical field [0001] The invention belongs to the fields of vascular biology and biomechanics, and relates to an experimental device for simulating the microenvironment of blood vessels in vitro. The invention can simulate the vascular microenvironment in vitro, and study the regulating mechanism of the vascular microenvironmental factors on the vascular structure and function. 2. Background technology [0002] Cardiovascular disease is a serious threat to human life and health. As far as coronary heart disease, cerebral apoplexy and hypertension are the three cardiovascular diseases with the highest incidence and the greatest harm, they are basically vascular diseases. At present, the general view is that blood vessels are not only blood channels, but also organs with complex functions. It can not only synthesize and secrete prostaglandins, endothelium-dependent relaxation factors, endothelium-dependent hyperpolarization factors, endothelin, angiotensin and a series ...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to design an experimental device for simulating the microenvironment of blood vessels in vitro, effectively solving the problem that existing devices cannot be used to study the interaction between blood (blood cells) flow-biochemical factors-blood vessels (and cells thereof), especially It is the disadvantage that it cannot be used to study the regulation mechanism of vascular microenvironmental factors on vascular structure and function

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