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Paecilomyces variotii bacterial strain SJ1 and application thereof

A technology of Paecilomyces varostii and strains, applied in applications, fungi, chemicals for biological control, etc., can solve the problems of accelerated species disappearance, water pollution, frequent occurrence of sand and dust, etc. The effect of microbial growth, promoting root growth, and promoting growth and development

Active Publication Date: 2015-07-01
山东蓬勃生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unrestrained pressure on the agricultural ecosystem results in the transfer of pressure to the entire ecosystem, leading to water pollution, frequent occurrence of sand and dust, accelerated species disappearance, etc.

Method used

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  • Paecilomyces variotii bacterial strain SJ1 and application thereof
  • Paecilomyces variotii bacterial strain SJ1 and application thereof
  • Paecilomyces variotii bacterial strain SJ1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Wanshi is to be green mold (Paecilomycess variotii) The separation and identification of the strain SJ1

[0022] (1) Separation of Endococcinius of Seabuckthorn

[0023] ① The healthy wild sea buckthorn samples are processed and washed immediately after collecting, then the roots are cut into small segments, rinsed with 75%alcohol, washed with 10%Pakistan disinfection solution for 18 minutes, and finally rinsed with fungal water 4 times;

[0024] ② Cut the above -mentioned anti -sterilized roots under the sterile conditions to 0.5cm long segments on a small section of Bangladesh red medium tablet and place it on the 28 ° C training box for 5 days.Silk, repeatedly separated and purified through the tablet (Bangladesh Red Cultivation), and finally obtained the mildew of Wanshi (Paecilomycess variotii) The strain SJ1; the formula and production method of Bangladesh red medium are as follows: 5g of protein 胨, 10g of glucose, 1g of potassium dihydrogen phosphate, magne...

Embodiment 2

[0029] Example 2 Wanfishi is to be green mold (Paecilomycess variotii) Breakthrough SJ1's Experimental Experimental Experiment

[0030] (1) Seabuckthorn endogenous fungal liquid fermentation culture:

[0031] Wan's plan to be separated from the endogenous fungus of the sea buckthorn is to be green mold (Paecilomycess variotii) The strain SJ1 receives a flat PDA medium, and is cultivated for 6 days at 25 ° C. The perforated agar digging block is inoculated in 250ml bottle bottle with a 50ml seed culture medium (for the PDA medium without agar). It is 28 ℃ at 28 ℃, Rotate the 120R / min to train on the bed for 3 days as a seed, with a 10%volume inocus and 150ml fermentation medium (fermentation medium: potato extract 1.0 L, yeast paste 1.0 g, protein 胨 3.0 g, glucose 15.0 g, glucose 15.0 g, Gel 17.0 G, Preparation of potato extract: Take 200 grams of potatoes, cut into small pieces, add 1000 ml of seawater to boil for 30 minutes, filter the potato pieces, and make up the filtrate to 1...

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Abstract

The invention discloses Paecilomyces variotii bacterial strain SJ1 and in particular relates to sea-buckthorn fungus, a preparation of sea-buckthorn endophytic fungi and extract of the sea-buckthorn fungus and use of the sea-buckthorn fungus extract. By utilizing the Paecilomyces variotii bacterial strain SJ1, growth and root development of crop, such as corn, rice are promoted, antifreezing capacity is improved, and yield is increased. The SJ1 bacterial strain is Paecilomyces variotii according to morphological and genetic analysis and identification.

Description

Technical field [0001] The present invention involves the field of biotechnology, and is specifically a Wan's plan to be green mold. (Paecilomycess variotii) Base SJ1 and its application. Background technique [0002] With the expansion of the population and the improvement of people's living standards, people's demand for agricultural products is increasing.China is a large population country, with a per capita arable land area of ​​less than 2 acres. The perennial plunder production and operation and backward technology make the agricultural ecosystem overwhelmed. It seriously affects the safety of food production in my country. As an important resource material, food cannot depend on the international market.Pressive pressure on the agricultural ecosystem is not restrained. As a result, the pressure was passed on to the entire ecosystem, which caused water pollution, frequent sand and dust, and accelerating the disappearance of species.In response to these situations, to accel...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/79
CPCA01N63/30C12N1/145C12R2001/79
Inventor 孔波程丽娜耿全政崔国庆程玲
Owner 山东蓬勃生物科技有限公司
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