158 results about "Paecilomyces sp" patented technology

The invention relates to an agricultural microbial fertilizer and a preparation method and application thereof. The agricultural microbial fertilizer comprises a medium and thalli. The thalli comprise bacillus subtilis, Bacillus megaterium, Bacillus mucilaginosus, Bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, Bacillus mucilaginosus, Streptomyces jingyangensis, azotobacter vinelandii, bacillus coagulans, Aspergillus oryzae and paecilomyces lilacinus. The agricultural microbial fertilizer provided by the invention can improve soil, can effectively control part of plant diseases and insect pests and can supply nutrients to soil to effectively solve a long-term problem of soil hardening. In addition, the agricultural microbial fertilizer can dissolve phosphorus and potassium so as to affectively release phosphorus, potassium, etc. existing in the form of a chelate in the soil.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention relates to a bacterial strain for preventing and treating tobacco black shank and the bacteria agent thereof, which is characterized in that: the invention adopts paecilomyces lilacinus ZY-19-2, the collection number is CCTCCNO: M207196, as the original bacterial strain to prepare the bacteria agent through a fermentation process, and the fermentation conditions are as follows: 0.8 to 2.0 percent of colloidal chitin is taken as a carbon source, the initial pH value of the fermentation fluid is 5.5 to 7.0, 0.8 to 1.5 percent peptone is taken as a nitrogen source, 0.1 percent Tween-80 is taken as a surfactant, the fermentation time is 48 to 72h, the vaccination amount is 0.8 to 2 percent, and the rotational speed of a shaking bed is 100 to 180r / min. The invention has the following advantages that: 1. the paecilomyces lilacinus strain has fast growth, the producing capacity of chitin is strong; 2. the invention can decompose the body walls of flies and maggots and can grow on the body walls of flies and maggots or a culture medium which is prepared by the decomposition products of the body walls and water; 3. the invention can be colonized at the root of a tobacco plant for resisting the infection of the tobacco black shank; 4. the invention can effectively overcome the antibacterial effect of the soil and maintain the suppression capacity of the tobacco black shank for a long time.

The invention provides a phosphate-solubilizing composite microorganism fungicide, which has effective components of pantoea ananatis, pythium oligandrum, trichoderma harzianum and paecilomyceslilacinus. According to the invention, high-efficiency phosphate-solubilizing bacterium-increase bacterium pantoea ananatis with wide applications and fungus of high-efficiency antagonistic crop disease areorganically combined, by applying the composite fungicide of the invention, while soil phosphorin is efficiently utilized and phosphatic fertilizer utilization rate is improved, the effect of antagonistic microorganisms in preventing soil-borne diseases can be sufficiently played, and the effects of solubilizing phosphate to increase yield and resisting diseases to increase yield are achieved. The phosphate-solubilizing composite microorganism fungicide has tremendous application value.

The present invention is one kind of organic biofertilizer capable of preventing and controlling nematode harm and its preparation process and use. The organic biofertilizer has the preparation process includes the following steps: 1. preparing materials for organic biofertilizer; 2. high temperature fermentation and decaying; 3. adding Paecilomyces lilacinus and culturing; 4. secondary fermentation; 5. palletizing; 6. quality inspection; and 7. packing. The organic biofertilizer capable of preventing and controlling nematode harm is suitable for use as base fertilizer, and has the long effect of reducing harm of nematode.

The invention discloses Paecilomyces lilacinus FZ07-9F-5 CGMCC No.2780, microbial inoculum and application thereof. The microbial inoculum is wireworm killing biological microbial inoculum with slow application function, which is prepared by the following steps: using a macromolecular material, namely sodium alginate as basic skeleton, using mycelium and spore mixture of P. lilacinus FZ07-9F-5 CGMCC No.2780 as active components, using infusorial earth as a carrier, and adopting a method of sodium alginate-calcium chloride reaction according to the characteristic of forming gel by the sodium alginate and polyvalent cations to be prepared into the microbial inoculum. Proved by experiments, the microbial inoculum has better wireworm killing efficacy, has control ratio up to between 50 and 60 percent, is comparatively stable, has no noxious chemical residues (innocuous and non-residue), does not pollute agricultural products and environment (does not react with other substances in the environment), and has the characteristics of high cost performance and convenient application. The microbial inoculum plays an important role in control of wireworm diseases of plant roots and knots, and has wide application prospect.

The invention belongs to the technical field of microorganism, in particular to a compound microbial preparation used for directly decomposing crop straws, namely an efficient straw-decomposing inoculant. The efficient straw-decomposing inoculant comprises the following raw materials by weight percentage: 20-30 percent of bacillus subtilis, 10-15 percent of white light gray streptomycete, 5-10 percent of endomycopsis fibuligera, 5-10 percent of candida utilis, 10-15 percent of aspergillus, 10-15 percent of paecilomyces lilacinus, 10-15 percent of trichoderma harzianum and 20-30 percent of thermophilus streptomycete. The efficient straw-decomposing inoculant is organically combined with soil no-tillage, shifting and fallow to achieve the comprehensive purposes of protecting the environment, improving soil, increasing the utilization rate of fertilizers, increasing the quantity and the quality of agricultural products and developing the ecological agriculture.

The invention discloses paecilomyces lilacinus and an application thereof. The provided paecilomyces lilacinus refers to paecilomyces lilacinus E16 with a register number of CGMCC No.5951 in the common microorganism center of the China microorganism culture preservation management committee. According to the paecilomyces lilacinus E16, in potting experiments, the control effect on second-instar banana root-knot nematodes is 86.37%, the growth rate to banana root weight is 37.76%, the growth rate to banana plant height is 56.47%, the growth rate to overground parts of banana is 38.03%; and in area experiments, the control effect on banana root-knot nematodes is 83.03% and the growth rate to banana fruit is 28.05%.

The invention discloses Paecilomyces variotii bacterial strain SJ1 and in particular relates to sea-buckthorn fungus, a preparation of sea-buckthorn endophytic fungi and extract of the sea-buckthorn fungus and use of the sea-buckthorn fungus extract. By utilizing the Paecilomyces variotii bacterial strain SJ1, growth and root development of crop, such as corn, rice are promoted, antifreezing capacity is improved, and yield is increased. The SJ1 bacterial strain is Paecilomyces variotii according to morphological and genetic analysis and identification.

The invention discloses a paecilomyces lilacinus strain and its culture method and use. The paecilomyces lilacinus strain is paecilomyces lilacinus CPLI0708, is preserved in the China general microbiological culture collection center (CGMCC) and has an accession number of CGMCC No.8450. The culture method of the paecilomyces lilacinus strain comprises the following steps of A, bacterial strain activation: carrying out bacterial strain streak cultivation activation on a PDA inclined plane, B, bacterial strain liquid fermentation culture: transferring the activated bacterial strain into a triangular flask with a liquid medium and carrying out shaking culture, and C, bacterial strain solid fermentation culture: mixing a carrier, a nutrition matrix and water according to a volume ratio to form a uniform mixture which is a solid fermentation medium, putting the solid fermentation medium into a fresh-keeping bag, inoculating the solid fermentation medium with a liquid fermentation seed, carrying out stirring to obtain a uniform mixture and carrying out culture. The bacterial strain conidiospore has strong effects of killing sucking-type insects such as greenhouse aphids and whitefly and the fermentation broth has strong effects of killing larvas of soybean cyst nematode and can be used for pollution-free vegetable production.

The invention discloses a ghost moth paecilomyces leavening feed additive. Ghost moth paecilomyces leavening is obtained in such a way that myceliums of a high-yield bacterial strain obtained by mutagenizing ghost moth paecilomyces separated from worm grass by ion beams are dried and crushed together with a a culture medium after strain preparation, shaking culture, primary starter can fermentation, fermentation can fermentation, solid culture fermentation. The invention also discloses an application of the ghost moth paecilomyces leavening feed additive in the preparation of animal feed, and the ghost moth paecilomyces leavening feed additive is added in the animal feed by 0.05 to 0.5 percent . The ghost moth paecilomyces leavening feed additive can increase the daily gain of animals, obviously promote the growth of the animals, improve the conversion rate of the feed, enhance the production performance and the antiviral capability of the animals and can also improve the quality of livestock and fowl meat. The ghost moth paecilomyces leavening has simple preparation technology flows, simple-formula culture medium and cheap and easily obtained raw materials.

The invention belongs to the field of biological prevention and control on crop insect damage and provides a microbial preparation for inhibiting root-knot nematode. The preparation is formed by mixing bacterial liquids of streptomyces avermitilis, paecilomyces lilacinus, verticillium chlamydosporium and Bacillus sphaericus and glycerol. The invention also provides a production method and an application effect of the microbial preparation for inhibiting the root-knot nematode. The microbial preparation comprises viable bacteria of which the number is greater than 2,500 millions per milliliter, wherein the viable bacteria comprise the streptomyces avermitilis, the paecilomyces lilacinus, the verticillium chlamydosporium and the Bacillus sphaericus. After the microbial preparation is prepared into soil, eggs of the root-knot nematode are killed by metabolites generated in the continuous growth and reproduction activity process of the viable bacteria, so that a granular structure of the soil is improved, the utilization rate of a fertilizer is improved and an effect of increasing both production and income is achieved.

The invention discloses paecilomyces javanicus strain and the application thereof. The strain adopts paecilomyces javanicus IJ-N2, the strain is preserved in the China Center for Type Culture Collection on tenth of June in 2008 with a CCTCC No of M208085. The strain is wild strain obtained through separating a plutella xylostella body naturally infested by entomogenous fungous from Nonggang Natural Forest Reserve Areas in Guangxi, the paecilomyces javanicus strain is obtained through the wild strain being back inoculated to the plutella xylostella body for rejuvenation, and depurative strain is obtained through the monospore separating operation being performed to the strain. The paecilomyces javanicus strain has strong infestation and insecticidal effect to aleyrodids, solenopsis invicta buren, spodoptera litura, aphid and plutella xylostella according to long-term infection biology study and indoor bioassay.

The invention discloses preparation and application of a soil ecosystem nursing microorganism bacterial fertilizer. The soil ecosystem nursing microorganism bacterial fertilizer contains abundant organic matters and beneficial bacteria, especially paecilomyces lilacinus. The preparation method for the fertilizer mainly comprises the following steps: preparation of a paecilomyces lilacinus strain; enlarged cultivation of a liquid strain used for production; preparation of a medium; addition of the paecilomyces lilacinus strain for enlarged cultivation; secondary fermentation; etc. The microorganism bacterial fertilizer is applied as a base fertilizer to soil according to an amount of 100 to 200 kg / mu, can improve soil, especially soil in a greenhouse, is capable of obviously mitigating harm of a plurality of plant nematode diseases caused by root-knot nematode, soybean cyst nematode, stem eelworm and the like, and alleviates continuous cropping obstacles.

The present invention provides a strain of entomopathogenic Beauveria bassiana, compositions comprising the entomopathogenic fungi strain or metabolites of the strain, and the use of the entomopathogenic fungi strain and compositions as biological control agents. Methods for the biological control of phytopathogenic insects using an entomopathogenic Beauveria bassiana fungi strain or one or more metabolites thereof, optionally together with other entomopathogenic fungi including fungi selected from strains of Lecanicillium spp., Paecilomyces fumosoroseus, and compositions comprising said fungi or metabolites thereof are also provided.

The invention relates to a method for producing cordyceps sinensis powder by virtue of liquid fermentation on mixed bacterial strains. The method comprises cultivation of bacterial strains, fermentation, solid-liquid separation, drying and smashing. The method for producing the cordyceps sinensis powder by virtue of the liquid fermentation on the mixed bacterial strain is characterized in that the fermented bacterial strains are hirsutella sinensis, hepialidae paecilomyces varioti and China cephalosporium. According to the method, three different bacterial strains, namely hirsutella sinensis, hepialidae paecilomyces varioti and China cephalosporium, are utilized, respective fermentation and growth characteristics of the three different bacterial strains are combined, the cordyceps sinensis powder which is closer to natural cordyceps sinensis in composition and has more effects is obtained by virtue of the liquid fermentation of the three different bacterial strains, and good economic benefit is brought for manual industrialization of cordyceps sinensis.

The present invention discloses a strain of Paecilomyces lilacinus for prevention and control of meloidogyne diseases of tomato and grape. The Paecilomyces lilacinus of the present invention is Paecilomyces lilacinus YT08, has the preservation number of CGMCC No.10026, is preserved in the China General Microbiological Culture Collection Center (CGMCC) in November 26, 2014, provides strong prevention and control effects for meloidogyne of tomato and grape, and can be developed as the novel biological prevention and control agent for the agriculture meloidogyne diseases.

The invention discloses a microbial preparation for preventing and controlling plant root knot nematodes and the application thereof. The preparation is mixed from Paecilomyces lilacinus, Bacillus subtilis, shell powder, shrimp shell powder, weathered coal humic acid and peat. The shell powder and shrimp shell powder are added to induce paecilomyces lilacinus to produce chitinase. The preparation can be stored at room temperature, and after storage for 90 days at 25 DEG C, the survival rate of paecilomyces lilacinus is more than 80%. The tomato pot experiment and cucumber field test prove that the preparation has remarkable effect on the control of root knot nematodes; at the same time, soil colonization test verify the Paecilomyces lilacinus in the preparation has good colonization condition in soil.

The invention provides a soil improvement formula. The soil improvement formula comprises a soil conditioner, a microbial agent and biostimulant, the soil conditioner is prepared from kitchen organicwaste, and the microbial agent is prepared from 5 wt%-10 wt% of actinomycetes, 10 wt%-20 wt% of nitrogen-fixing bacteria, 5 wt%-10 wt% of paecilomyces lilacinus, 10 wt%-25 wt% of trichoderma viride, 5wt%-20 wt% of lactic acid bacteria and 20 wt%-35 wt% of bacillus subtilis. The biostimulant comprises seaweed extract and / or crustacean extract, wherein the mass ratio of the soil conditioner to themicrobial agent to the biostimulant is (300-800): (150-200): (60-120). According to the soil improvement formula, the secondary metabolism level of apples can be increased, and the external light color and flavor of fruits are improved. The invention further provides an application of the soil improvement formula in apple planting.

The invention discloses a heavy metal bioremediation agent containing alga oligosaccharides and a preparing method of the agent, and relates to the technical field of heavy metal bioremediation application. The heavy metal bioremediation agent containing the alga oligosaccharides comprises, by mass percentage, 5% to 15% of the alga oligosaccharides, 10% to 20% of bacillus subtilis fermented liquid and 65% to 85% of paecilomyces lilacinus solid leavening, wherein the effective viable count is larger than or equal to 2 hundred million CFU / g. The preparation method comprises the steps that the bacillus subtilis fermented liquid is prepared; the paecilomyces lilacinus solid leavening is prepared; and the alga oligosaccharides, the bacillus subtilis fermented liquid obtained in the first step and the paecilomyces lilacinus solid leavening obtained in the second step are mixed, and the heavy metal bioremediation agent containing the alga oligosaccharides is obtained. Bacillus subtilis and paecilomyces lilacinus have the higher tolerance on multiple kinds of heavy metal and can directly adsorb heavy metal ions, and the bacillus subtilis and the paecilomyces lilacinus do combined action with the alga oligosaccharides so as to be capable of passivating the heavy metal in the soil. Preparation is simple, the repairing effect on the heavy metal in the soil is good, and safety and environmental protection are achieved.

The invention belongs to the technical field of microbial fermentation, and specifically relates to media for producing Paecilomyces cicadae conidiospores, a culture method thereof, a culture product thereof and application thereof. The media for producing Paecilomyces cicadae conidiospores comprise a solid medium and a liquid medium. The culture method provided in the invention can produce considerable Paecilomyces cicadae conidiospores; the culture product of the method comprises components like polysaccharides, cordycepic acid, adenosine, triterpenoids and a plurality of amino acids, has the functions of regulating immunity, relieving fever and pain, improving renal function, lowering blood pressure, resisting radiation and tumor, reducing blood sugar, etc., and is applicable to the field of human healthy life; considerable spores are generated in the process of fermentation and the generated spores can be used as effective means of biological control, thereby solving the problem that the amount pesticide residues in agricultural products exceed the standard amount from the head stream.

The invention discloses a paecilomyces lilacinus fungal spore oil suspending agent and a preparation method as well as application thereof, belonging to the technical field of pesticides. The suspending agent is prepared from the following components in percentage by weight: 1-20 percent of paecilomyces lilacinus fungal spore powder, 10-30 percent of emulsifier, 2-10 percent of thickening agent, 3-15 percent of dispersant and 25-84 percent of plant oil. The preparation method comprises the following step of: stirring the various components weighed according to the proportion in a stirring machine under the condition of environmental temperature of not higher than 30 DEG C for 0.5-1 hour, grinding the various components weighed according to the proportion in a sand mill at the rotation speed of 1,000-15,000 r / min for 10-20 minutes or shearing the various components weighed according to the proportion in a high shear at the rotation speed of 10,000-50,000 r / min for 5-10 minutes to obtain the paecilomyces lilacinus fungal spore oil suspending agent. The suspending agent has the advantages of obvious prevention and treatment effect on nematode, long duration of pesticide effect, environmental friendliness, safety and capability of promoting plant growth.

The invention relates to an application of an entomogenous fungi liquid fermentation mycelium as a shrimp feed additive, and belongs to the technical field of feed. The entomogenous fungi liquid fermentation mycelium is a Hirsutella sinensis liquid fermentation mycelium, a Paecilomyces gunnii liquid fermentation mycelium, or a Paecilomyces militaris liquid fermentation mycelium. The entomogenous fungi liquid fermentation mycelium is used for improving the growth indexes such as the survival rate, the weight gain rate and the feed utilization rate of white shrimps and increasing immunity.

The invention relates to the technical field of medicine, in particular to a method for preparing a ginsenoside Rd by using the microbial transformation. The invention provides a method for preparinga ginsenoside Rd by using the microbial transformation, including the following steps: adding the ginsenoside into a nutrient solution, and fermenting after Paecilomyces sp being inoculated; obtainingthallines, adding alcohol liquid into the thallines to extract, and obtaining alcohol extract; extracting the alcohol extract with saturated n-butanol; and separating and purifying by chromatographiccolumn. The method disclosed by the invention has high conversion rate, which reaches 70 to 90%, the fermented product ginsenoside Rd has the purity of 98%, moreover, the microbial industrializationpreparation of ginsenoside Rd can be realized, the medicament resource source of ginsenoside Rd can be expanded, and the wide health care market requirement can be met.

The invention discloses a profitable soil microorganism mixed fermentation method. The method includes the steps of 1) activating paecilomyces lilacinus, issatchenkia orientalis and bacillus licheniformis on a PDA (potato dextrose agar) medium, a YPD (yeast extract peptone dextrose) medium and a nutrient agar medium respectively, and transferring the three activated strains to corresponding liquid media so as to prepare seed fermentation broth; 2) inoculating 10% of each of the three strains into a mixed fermentation medium, and controlling the living bacteria count of the bacillus licheniformis to be one order of magnitude higher than those of the paecilomyces lilacinus and the issatchenkia orientalis according to the relation between the living bacteria count of the seed fermentation broth and an OD600 (optical density 600) value. The profitable soil microorganism mixed fermentation method has the advantages that a mixed fermentation medium formula is simple, production cost is reduced, equipment utilization rate is increased, and accordingly the profitable soil microorganism mixed fermentation method is suitable for large-scale production of fermented products; the fermented products are capable of improving soil flora balance, promoting growth and increasing production and can be used for producing biofertilizer and biopesticide by being compounded with organic materials, soil improvement agents and the like.

The invention discloses epipodium polycyclic aromatic hydrocarbons degrading bacteria and the application thereof. The epipodium polycyclic aromatic hydrocarbons degrading bacteria (paecilomyces variotii) F6 provided by the invention has the function of pipodium polycyclic aromatic hydrocarbons and pipodium polycyclic aromatic hydrocarbons mixture. After the pipodium polycyclic aromatic hydrocarbons is cultured for 30 d in a polycyclic aromatic hydrocarbons compound system, the degrading rate to the polycyclic aromatic hydrocarbons becomes 16.06 percent to 24.57 percent; however, the degrading rate in a single polycyclic aromatic hydrocarbons compound system becomes 10.42 percent to 33.25 percent, wherein, the degrading rate of benzo (k) fluoranthene and benzo (b) fluoranthene is the highest, which respectively accounts for 33.25 percent and 26.44 percent. The strain provided by the invention can supply novel germplasm resource for developing the co-metabolism mechanism study of a plurality of epipodium polycyclic aromatic hydrocarbons and the construction of aromatic hydrocarbons combined pollution water-soil environment repairing technology.

The invention provides an endophytic fungus (Paecilomycessp.) capable of accelerating phosphorus absorption of cedar and a separation, purification, inoculation, cultivation and application method thereof. The endophytic fungus is (Paecilomycessp.)AJ6 and is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCCNO.9186. The endophytic fungus bacterial strain related by the invention is separated and purified from a cedar plant, and is inoculated to a cedar tissue culture seedling, according to comprehensive evaluation of indicators of photosynthesis, the endophytic fungus is further proven to have the effect of accelerating phosphorous absorption of the cedar, functional endophytic fungus beneficial to acceleration of phosphorous absorption of the plant is found, and the functional endophytic fungus can be applied to production.

The invention provides biological fertilizer and a preparation method and application thereof and belongs to the technical field of biological organic fertilizer. The biological fertilizer is preparedfrom, by mass, 30-45 parts of pig manure, 10-25 parts of seaweed, 20-35 parts of mushroom residues, 5-20 parts of straw, 10-30 parts of paecilomyces lilacinus and 5-15 parts of Bacillus licheiformis,wherein the number of effective viable bacteria in the paecilomyces lilacinus is greater than or equal to 120 million / mL. The number of effective viable bacteria in the Bacillus licheiformis is greater than or equal to 80 million / mL. The provided biological fertilizer can improve the disease resistance of crops and has a good inhibition effect on root knot nematode diseases of the crops and certain effects of improving the quality of the crops and increasing the yield of the crops.

The present invention belongs to the technical field of biological pesticides, and specifically discloses paecilomyces lilacinus PlTS01 strain and application thereof in bemisia tabaci control. The paecilomyces lilacinus PlTS01 strain is isolated and purified from soil in Zhongchuan town Yongdeng County in Gansu Province, and is preserved in China Center for Type Culture Collection (CCTCC) on March 31, 2015, and the accession number is CCTCC No. M2015168. The paecilomyces lilacinus PlTS01 strain has strong bemisia tabaci insecticidal activity, sixth day after the treatment, medial lethal concentration LC50 of B-type bemisia tabaci second instar nymphs is 1.66 * 10<6> spores / mL, and the median lethal time LT50 is 4.35 d (at a dose of 1.0 *10<7> spores / mL). The paecilomyces lilacinus PlTS01 strain shows good prospects for being used as an environmentally friendly microbial pesticide in control of the agricultural important pest-bemisia tabaci.