63 results about "Hirsutella sp." patented technology

The invention discloses a solid fermentation method for cordyceps sinensis. The method adopts fermented liquor the seed lot of which is Hirsutella sinensis Liu, Guo, Yu et Zeng as a liquid strain to enable cordyceps sinensis to be fermented and produced on a solid culture medium taking grains as the matrix by pouring or blending inoculation. The method combines the advantages of both liquid fermentation and solid fermentation. The method has the advantages of short strain cultivating time, high activity and good flowability and many development points and has the characteristics of small investment of production equipment, low energy consumption, no waste liquor discharge, easy storage of products and the like. The final product of the invention is the grain being rich in cordyceps sinensis and can be directly used as raw material of health-care food.

The invention relates to a method for producing cordyceps sinensis powder by virtue of liquid fermentation on mixed bacterial strains. The method comprises cultivation of bacterial strains, fermentation, solid-liquid separation, drying and smashing. The method for producing the cordyceps sinensis powder by virtue of the liquid fermentation on the mixed bacterial strain is characterized in that the fermented bacterial strains are hirsutella sinensis, hepialidae paecilomyces varioti and China cephalosporium. According to the method, three different bacterial strains, namely hirsutella sinensis, hepialidae paecilomyces varioti and China cephalosporium, are utilized, respective fermentation and growth characteristics of the three different bacterial strains are combined, the cordyceps sinensis powder which is closer to natural cordyceps sinensis in composition and has more effects is obtained by virtue of the liquid fermentation of the three different bacterial strains, and good economic benefit is brought for manual industrialization of cordyceps sinensis.

The invention relates to IMP (inosine monophosphate) dehydrogenase synthesized by 'Bailing' producing fungus Chinese caterpillar fungus hirsutella sinensis to metabolize guanine nucleotide based on xanthosine monophosphate and coding genes and application of the IMP dehydrogenase. The IMP dehydrogenase comprises proteins shown by SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3, and coding genes of the IMP dehydrogenase correspond to nucleotide sequences shown by SEQ ID No.4, SEQ ID No.5 and SEQ ID No.6. Detailed studies on metabolic pathways of the xanthosine monophosphate synthesizing the guanine nucleotide in principle are carried out and include that cloned DNAs (deoxyribonucleic acids) in the provided nucleotide sequences can be transferred into engineering bacteria by means of transduction, transformation and transconjunction, and high expressivity is given to the host guanine nucleotide by adjusting expressions of guanine nucleotide biosynthetic genes, so that an effective way for increasing output of the guanine nucleotide is provided, and the IMP dehydrogenase and the coding genes have significant application prospects.

The invention belongs to the medicine, processing and healthcare food technical field of cordyceps sinensis, particularly relating to a preparation method of the cordyceps sinensis extractive concentrated solution got by hirsrtella sinensis or hirsutella hepialid and applications of the cordyceps sinensis extractive concentrated solution in preparation of various preparation forms of medicine or healthcare products for strengthening and regulating immunity; thereby the solution can be applied for treating deficiency in lung and kidney, cancers and for helping strengthening immunity after themotherapy. The preparation of the invention is that the hirsrtella sinensis is fermented to produce cordyceps sinensis which is extracted respectively by ethanol, water and zymohydrolysis and then the extractive is condensed into concentrated solution which is produced into various preparation forms by spray-drying or directly produced into soft capsules after sterilization, and then the finished product is prepared well after packing and inspecting. No additive is added into the finished product which is composed of the extractive concentrated solution by 100 per cent. The various preparations prepared by the extractive concentrated solution of cordyceps sinensis all have multiple functions of restoring the deficiency in lung and kidney, strengthening immunity and enhancing resistance and have the function of regulating and steadying the immunological system of a human body, not only applicable to sub-healthy people for regular health care but also evident in efficacy for people who takes organ transplantation, cancer radiotherapy and chemotherapy, pneumonia, hepatitis, chronic nephritis and valetudinarianism.

The invention relates to an application of an entomogenous fungi liquid fermentation mycelium as a shrimp feed additive, and belongs to the technical field of feed. The entomogenous fungi liquid fermentation mycelium is a Hirsutella sinensis liquid fermentation mycelium, a Paecilomyces gunnii liquid fermentation mycelium, or a Paecilomyces militaris liquid fermentation mycelium. The entomogenous fungi liquid fermentation mycelium is used for improving the growth indexes such as the survival rate, the weight gain rate and the feed utilization rate of white shrimps and increasing immunity.

The invention relates to long-chain-acyl-coenzyme A synthetase for synthesizing metabolic cetyl-coenzyme A with the start that Bailing producing strain cordyceps sinensis hirsutella sinensis is participated in palmatic acid, a gene for coding the enzyme and an application thereof. The amino acid sequence of the long-chain-acyl-coenzyme A synthetase and the sequence represented by SEQ ID No. 1 or SEQ ID No. 3 have more than 90% of homology. According to the invention, the metabolic pathway for synthesizing cetyl-coenzyme A through palmatic acid is researched in principle in a detailed manner as follows: clone DNA of a nucleotide sequence provided by the invention can be transferred into engineering bacteria through transduction, conversion and combined transfer methods; gene expression is biologically synthesized by adjusting the cetyl-coenzyme A; high expressivity of host long-chain-acyl-coenzyme A synthetase is given; an effective way for enlarging the yield of the cetyl-coenzyme A is provided; and the cetyl-coenzyme A has a significant application prospect.

The invention discloses a cordyceps sinensis fungal fermentation liquid ferment and a preparation method thereof. The cordyceps sinensis fungal fermentation liquid ferment comprises the following raw materials by weight percent: 1-99 percent of hirsutella sinensis fermentation liquid, 0-98 percent of fruits and / or vegetables and / or traditional Chinese medicinal materials or 0-98% of fungal and probiotics and 1-99 percent of sugar. The ferment is obtained by uniformly mixing the raw materials according to the weight percent under germfree, drying and sealing conditions and fermenting at the temperature of 0-55 DEG C for more than 3 months. The cordyceps sinensis fungal fermentation liquid ferment prepared by the method disclosed by the invention can be directly filled and drunk, can be also redried to obtain ferment powder used as food, cosmetics and medicine additives, and can be also processed into various preparations for direct taking. The cordyceps sinensis fungal fermentation liquid ferment has rich active components; furthermore, the invention also provides an effective hirsutella sinensis fermentation liquid recycling and using way which has actual economic and social value.

The invention relates to a culture method based on a hirsutella sinensis metabolic pathway. The method comprises the following steps: inoculating the hirsutella sinensis strain into a fermentation culture medium, wherein a key differential metabolite is added into the fermentation culture medium, performing culture at 10-25 DEG C and 100-250 rpm for 216-240 h, performing filtering, oven-drying andgrinding into a powder on a fermentation product after the fermentation is completed, and then extracting cordycepic acid and cordycepin. The method can effectively increase the content of main active compounds cordycepic acid and cordycepin through adding various key differential metabolites before fermentation culture of hirsutella sinensis. As can be seen from the embodiment of the invention,the addition of sorbitol in the culture medium can increase the content of cordycepic acid from 59.28 mg / g to 149.38 mg / g; and the addition of L-proline or trans-4-hydroxy-L-proline can increase the content of cordycepin from 0.1103 mg / g to 0.1622 mg / g or 0.1867 mg / g, respectively.

The invention relates to a purine nucleosidase participating in purine-nucleoside-initiated anabolism of purine such as adenine, guanine, hypoxanthine, xanthine, and the like. The purine nucleosidase is obtained from a Bailing production fungus which is cordyceps sinensis hirsutella sinensis. The invention also relates to a purine nucleosidase coding gene, and an application of the purine nucleosidase. The amino acid sequence of the purine nucleosidase is represented as SEQ ID No.1. The nucleotide sequence of the coding gene is represented as SEQ ID No.2. From the respective of the principle, the invention makes detailed researches in the metabolic pathway in purine synthesis of purine nucleoside. The cloned DNA of the nucleotide sequence provided by the invention can be transferred into engineering fungus through methods of transduction, transformation, and conjugation transferring. Through the regulation upon the expression of biosynthesis gene of purine, high-expression is provided for host purine, such that an effective way for increasing purine yield is provided. Therefore, the invention has important application prospect.

The invention discloses a method for fermenting hirsutella sinensis, which comprises the following steps: inoculating a seed solution of the hirsutella sinensis into an airlift fermentation tank filled with a fermentation culture medium according to the weight ratio of 10-20%, fermenting and culturing at the temperature of 15-20 DEG C under the conditions that the ventilation ratio is 1: (0.5-1.5) vvm and the tank pressure is 0.02-0.05 Mpa, and feeding and supplementing the culture medium, glucose and hydrogen in the middle and later periods of fermentation. The airlift fermentation tank is adopted to reduce damage of shearing force to mycelia, the thallus growth activity is good, and the reducing sugar metabolism rate is high. On one hand, substrate feedback inhibition is relieved and the delay phase is shortened by adopting a full-nutrition fed-batch mode; on the other hand, nutrient substances are timely supplemented in the middle and later periods of fermentation, and the thallus growth time is prolonged. The utilization rate of the substrate is improved by utilizing glucose online control. Compared with a traditional method, the yield is increased, the mycelium quality is improved, and the content of effective active ingredients in the mycelium is remarkably increased.

The invention relates to an inosine monophosphate (IMP) dehydrogenase from 'Bailing' producing strain Cordyceps sinensis Hirsutella sinensis for participating in anabolism on IMP to obtain a xanthylic acid, a gene for encoding the dehydrogenase and application of the dehydrogenase. The IMP dehydrogenase comprises a protein shown by SEQ ID No.1 and a protein shown by SEQ ID No.2, and the coding gene of the protein shown by SEQ ID No.1 and the coding gene of the protein shown by SEQ ID No.2 respectively correspond to a nucleotide sequence shown by SEQ ID No.3 and a nucleotide sequence shown by SEQ ID No.4. According to the invention, the metabolic pathway of the IMP to synthetize the xanthylic acid is traversed from the principle; the cloning deoxyribonucleic acids (DNA) of the nucleotide sequences provided by the invention can be transferred into engineering bacteria through a transduction method, a transformation method and a conjugal transfer method; and through regulating the expression of the biosynthetic gene of the xanthylic acid, the high expressivity of a host xanthylic acid is endowed, and an effective way for increasing the yield of the xanthylic acid is provided. The IMP dehydrogenase has a great application prospect.

The invention relates to a guanine deaminase for synthesizing and metabolizing an xanthine from guanine of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis from 'Bailing' production strain, a gene coding the enzyme and application of the guanine deaminase. The guanine deaminase comprises the proteins shown in SEQ ID No. 1 and SEQ ID No. 2, and the coding gene of the guanine deaminase corresponds to the nucleotide sequence shown in SEQ ID No. 3 and SEQ ID No.4. According to the invention, the metabolizing way for synthesizing the xanthine from the guanine is researched in detail from the principle, comprising: the cloned DNA of the nucleotide sequence provided by the invention can be transferred to an engineering strain by transduction, transformation, and combined transfer; the expression of the gene is biologically synthesized by regulating the xanthine, the host xanthine is given high expressivity, an effective way is provided to increase the yield of the xanthine, and the invention has a great application prospect.

The invention relates to an enzyme from Bailing production bacterium Cordyceps Chinese Hirsutella for synthesizing metabolic (desoxy) uridine from (desoxy) cytidine, a gene for coding the enzyme and application thereof. The enzyme is cytidine deaminase which has more than 90% of homology with amino acid sequence disclosed as SEQ ID NO.1; and the coding gene has more than 90% of homology with nucleotide sequence disclosed as SEQ ID NO.2. The invention researches the metabolic pathway of (desoxy) cytidine synthesized (desoxy) uridine in details in principle. The cloned DNA (deoxyribonucleic acid) comprising the nucleotide sequence provided by the invention can be transformed into engineering bacterium by transduction, conversion and conjugal transfer. By adjusting the expression of the (desoxy) uridine biosynthesized gene, the host (desoxy) uridine is endowed with high expressivity, thereby providing an effective way for enhancing the yield of the (desoxy) uridine and having great application prospects.

The invention relates to a delta-5-desaturase from a Corbrin-production bacterium cordyceps sinensis hirsutella sinensis. The delta-5-desaturase participates in eicosapentaenoic coenzyme A anabolism started from eicosatetraenoic coenzyme A. The invention also relates to a gene coding the enzyme, and an application thereof. The amino acid sequence of the delta-5-desaturase has more than 90% homology with a sequence represented by SEQ ID No.1 or SEQ ID No.3. From the principle, the invention carries out detailed researches upon the metabolic pathway of eicosatetraenoic coenzyme A to synthesizing the eicosapentaenoic coenzyme A. A cloned DNA comprising the nucleotide sequence provided by the invention can be transferred to engineering bacteria through methods of transduction, transformation, and combined transferring. Through the regulation upon eicosapentaenoic coenzyme A biosynthetic gene expression, high expression of a host delta-5-desaturase is provided, an effective approach is provided for increasing eicosapentaenoic acid yield. The application has an important application prospect.

The invention relates to the field of cordyceps sinensis, and particularly discloses a method for improving the quality of fermented cordyceps sinensis mycelia, which comprises the following steps: S1, slant culture, S2, shake-flask culture, S3, seed amplification culture, S4, fermentation culture, and S5, fermentation zone reculture. Components such as vitamins, jerusalem artichoke, folic acid, nucleoside precursors, brown algae liquid, cicada pollen and vegetable oil are added on the basis of an original fermentation culture medium; in addition, corn flour and silkworm chrysalis meal in the culture medium are subjected to enzymolysis in advance through a hydrolysis method; therefore, the content of nucleoside substances, D-mannitol, polysaccharide, fatty acid, ergosterol, gamma-aminobutyric acid and other active ingredients in fermented Hirsutella sinensis mycelia is increased; and the drug effect of artificially fermented cordyceps sinensis is enhanced.

The invention relating to a method for preparation and utility of hirsutella polyspora fungi straying wireworm belongs to biotic pesticide technical sphere. The production strain is: Hirsutella polyspora ZK10, prepared in conventional method, and the formulation of culture medium is: yeast extract of 1 to 2 percent, peptone of 0.2 to 2 percent; dextroglucose of 2 to 4 percent; agar of 2 percent; and PH spontaneous. Prepare the lawn plate for testing with the said culture medium using the culture dish with a diameter of 9 cm in the conventional method, and it can be used when spore is produced largely. The strain carrys its point of wireworm prevention by straying wireworm, and the product has a high straying rate to pine beam wireworm and Panagrellus redivivus wireworm, having a good latent prospect for application.

The invention relates to a preparation and regeneration method for hirsutella sinensis protoplast. The method comprises the following steps of: taking a potassium chloride solution to serve as an osmotic pressure stabilizing agent, taking commodity enzymes Driselase from Basidiomycetes sp, Lysing Enzyme from Trichoderma harizianum and Yatalase as compound enzymes to carry out enzymolysis so as toprepare the hirsutella sinensis protoplast with a high content, and successfully regenerating. Meanwhile, a regeneration medium is optimized to shorten protoplast regeneration time, and in addition, the preparation and regeneration method is simple to operate, can be conveniently prompted and implemented and has a good application prospect.

The invention discloses hirsutella sinensis hypolipidemic healthcare beverage and a preparation method thereof. The active ingredient in the beverage is non-filtered or filtered hirsutella sinensis fermentation liquor. The preparation method of the fermentation liquor comprises the following steps: extracting a traditional Chinese medicinal herb consisting of 10 to 40 parts by weight of glossy privet fruit, 10 to 40 parts by weight of mulberry leaves, 10 to 40 parts by weight of gynostemma pentaphylla and 10 to 40 parts by weight of epimedium through an aqueous solution to obtain traditional Chinese medicinal composite extract liquor; preparing a fermentation culture medium by adopting the traditional Chinese medicinal composite extract liquor, turtle oil, Chinese torreya oil, glucose and water; inoculating the hirsutella sinensis strain into the fermentation culture medium, carrying out liquid fermentation to obtain the hirsutella sinensis fermentation liquor. The beverage contains the natural healthcare components such as turtle oil, Chinese torreya oil, glossy privet fruit, mulberry leaves, gynostemma pentaphylla and epimedium, which are matched with the fermented hirsutella sinensis fermentation liquor, so that a remarkable hypolipidemic function can be achieved. The preparation method of the beverage comprises the steps of uniformly mixing raw materials, and filtering and sterilizing the mixture. The method is simple to operate and the industrialized production can be realized easily.

The invention discloses fructokinase from a fungal traditional Chinese medicine and a coding gene and use thereof. The fungal traditional Chinese medicine fructokinase has an amino acid sequence as shown in SEQ ID NO: 1 and a nucleotide sequence of the coding gene as shown in SEQ ID NO: 2. The fungal traditional Chinese medicine fructokinase participates in synthesis of main precursor monosaccharide D-fructose of cordyceps polysaccharide from D-fructose-6-phosphoric acid. A fungal traditional Chinese medicine hirsutella sinensis polysaccharide biosynthetic metabolism pathway is studied in details in principle. A cloned DNA containing the nucleotide sequence can be transferred into engineering bacteria by transduction, transformation and conjugal transfer methods. An expression of a D-fructose biosynthetic gene is regulated, the host is endowed with high expressivity of D-fructose, an effective way is provided for increasing the yield of the D-fructose and a derivative thereof, and the fructokinase has a great application prospect.

The invention belongs to the technical field of agriculture, and particularly relates to a water-soluble fertilizer synergist and a preparation method thereof. The water-soluble fertilizer synergist is a dried product of fermentation liquor obtained by performing submerged fermentation on cordyceps fungus strains and then performing solid-liquid separation. The strain is one of Hirsutella sinensis, Paecilomyces hepiali (PaecilomyceshepialiQ.T.Chen&R.Q.Dai), Paecilomycesmilitaris, Acremonium terricola, and Cordyceps hawkesii Gray. The synergist has the advantages that (1) the utilization rate of the water-soluble fertilizer is promoted; (2) the application amount of the water-soluble fertilizer is reduced; (3) the effect is stable, and no toxicity or residue exists.

The invention relates to an enzyme from Bailing production bacterium Cordyceps Chinese Hirsutella for synthesizing metabolic pyrimidine nucleotide from pyrimidine nucleoside, a gene for coding the enzyme and application thereof. The enzyme is uridine-cytidine kinase which has more than 90% of homology with amino acid sequence disclosed as SEQ ID NO.1; and the coding gene has more than 90% of homology with nucleotide sequence disclosed as SEQ ID NO.2. The invention researches the metabolic pathway of pyrimidine nucleoside synthesized pyrimidine nucleotide in details in principle. The cloned DNA (deoxyribonucleic acid) comprising the nucleotide sequence provided by the invention can be transformed into engineering bacterium by transduction, conversion and conjugal transfer. By adjusting the expression of the pyrimidine nucleotide biosynthesized gene, the host pyrimidine nucleotide is endowed with high expressivity, thereby providing an effective way for enhancing the yield of the pyrimidine nucleotide and having great application prospects.

The invention discloses a strain of Ophiocordyceps sinensis and its application. The Ophiocordyceps sinensis KD11-2 has a preservation number of GDMCC No: 60594. The Ophiocordyceps sinensis KD11-2 ofthe present invention can grow sporophore on an artificial medium and form ascocarp (ascospores), and has the outstanding ability to form ascospores, under artificial cultivation condition, the Ophiocordyceps sinensis KD11-2 is used to produce and collect Ophiocordyceps sinensis ascospores, and the artificial cultivation of the Ophiocordyceps sinensis sporophores can be promoted, which provides anew method for utilizing the obtained ascospores to infect the larvae of host Hepialus armoricanus of Cordyceps sinensis.

The invention belongs to the technical field of agriculture, and particularly relates to a feed additive composition containing a hirsutella sinensis culture and a preparation method of the feed additive composition. The feed additive composition is prepared from the following components of a hirsutella sinensis [Hirsutella sinensis Liu, Guo, Yu, et Zang] fermentation culture, a jerusalem artichoke concentrated solution and a corn and silkworm chrysalis hydrolysis concentrated solution. The preparation method has the advantages that (1) the animal production performance and the product quality can be improved; (2) no toxic or side effect is generated after long-term use; (3) the feed additive composition can effectively enter gastrointestinal tracts to play a role, and the palatability of the feed is not influenced. and (4) incompatibility does not occur or rarely occurs, and bacteria are not easy to generate drug resistance to the composition.

The invention belongs to the technical field of agriculture, and relates to a feed additive containing hirsutella sinensis culture and a preparation method of the feed additive. The feed additive is a dried product of a fermentation broth obtained by carrying out liquid submerged fermentation and solid-liquid separation on the hirsutella sinensis [Hirsutella sinensis Liu, Guo, Yu, et Zang] strain. The method has the following advantages that: (1) the animal production performance and the product quality can be improved; (2) no harm is caused to human health, and excrement of animals does not cause potential pollution harm to the environment; and (3) the feed additive can effectively enter gastrointestinal tracts to play a role, and the palatability of a feed is not influenced. and (4) incompatibility does not occur or rarely occurs, and bacteria are not easy to generate drug resistance to the feed additive.

The invention discloses a method for improving the fermentation efficiency of hirsutella sinensis by using an oxygen carrier, which is characterized in that the oxygen carrier is used in the fermentation process, so that the oxygen utilization efficiency of thalli is improved, the yield of artificially cultured hirsutella sinensis thalli and the yield of substances such as cordycepin and adenosine are higher, the fermentation cost is reduced, and the emission of wastewater and waste gas is reduced. The oxygen carrier comprises substances such as n-dodecane, n-hexadecane, perfluorodecahydronaphthalene, olive oil and the like, and has the advantages of being small in dosage, convenient to obtain, capable of being recycled and reused, not absorbed and utilized by thalli and the like. The final thallus yield can reach up to 30.7%, the adenosine yield is 2.92 mg / g, the specific oxygen uptake rate (QO2) and the oxygen uptake rate (R) are obviously improved, the air oxygen critical concentration is obviously reduced, the power consumption is obviously reduced, the use of water and electricity is greatly reduced, the fermentation period is shorter, the efficiency is higher, and the product competitiveness of hirsutella sinensis produced by a fermentation method is improved.

The invention relates to an enzyme of metabolic adenine nucleotide synthesized by cordyceps sinensis hirsutella sinensis from bailing producing bacteria to participate in adenosine starting, gene coding the enzyme and an application of the enzyme. The adenosine kinase amino acid sequence is shown as SEQ ID No (sequencer identifier number).1, and the coding gene correspondingly has the nucleotide sequence shown as SEQ ID No .2. The metabolic pathway of the adenosine synthesized adenine nucleotide is detailedly studied in the principle aspect, cloning DNA (deoxyribonucleic acid) containing the nucleotide sequence provided by the invention can be used for being transferred into engineering bacteria by transduction, conversion and combination methods, high expression of host adenine nucleotide is given through regulating the adenine nucleotide biosynthetic gene expression, an effective path is provided for the yield increase of the adenine nucleotide, and great application prospects are realized.

The invention discloses a special culture medium for hirsutella sinensis and a preparation method thereof, and belongs to the field of biotechnology. 1000 mL of the culture medium is mainly prepared from 400 mL of beef juice, 200 mL of fresh milk, 0.5 g of compound vitamin B, 1 g of dipotassium phosphate, 0.5 g of sodium chloride, 0.5 g of biotin, 5 g of lactoalbumin hydrolysate, 0.5 g of nucleic acid, 1 g of yeast powder, 16 g of agar and 400 mL of water. The culture medium disclosed by the invention has the advantages of short delay phase of the inoculated hirsutella sinensis, rapid growth, robust hypha, stable hypha character, stable yield and the like, and is a very mature and stable culture medium formula in the research of the hirsutella sinensis.

The invention discloses a recombinant plasmid, which is characterized in that promoters, pGpdA and Trpc, in a plasmid pRFHUE-eGFP are respectively replaced by promoters, pAT and pTrpc, in a plasmid of entomogenous fungus. The invention also discloses a preparation method and an application of the recombinant plasmid, and discloses engineering bacteria that include the recombinant plasmid, as well as a preparation method and an application thereof. In the invention, the recombinant plasmid pRF-AETH as well as the engineering bacteria comprising same can convert Hirsutella sinensis, wherein blastospore and mycelia with strong green fluorescence of a conversion strain of the Hirsutella sinensis can be observed under a fluorescence microscope; this phenomenon indicates that in the recombinant plasmid pRF-AETH, an egfp gene can be effectively expressed in the Hirsutella sinensis, so that the recombinant plasmid can be applied to researches on growth, reproduction, development and differentiation processes of the Hirsutella sinensis. The recombinant plasmid supplies support to artificial cultivation of Cordyceps sinensis and has great application prospect.