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Method for improving fermentation efficiency of hirsutella sinensis by using oxygen carrier

A technology of Chinese hairy sporum and fermentation efficiency, which is applied in the field of bioengineering, can solve the problems of incompatibility between oxygen mass transfer efficiency and product quality, and achieve the effects of improving equipment utilization, reducing industrial costs, and reducing energy loss

Pending Publication Date: 2022-04-29
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a liquid fermentation culture method for high-efficiency oxygen transfer of Mortierella chinensis, aiming to solve the problem that the oxygen mass transfer efficiency and product quality cannot be compatible during traditional Mortierella Chinese fermentation, and to provide a Efficient and orderly, high product quality method of using oxygen carrier to improve the fermentation efficiency of Trichosporum sinensis

Method used

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  • Method for improving fermentation efficiency of hirsutella sinensis by using oxygen carrier
  • Method for improving fermentation efficiency of hirsutella sinensis by using oxygen carrier
  • Method for improving fermentation efficiency of hirsutella sinensis by using oxygen carrier

Examples

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Embodiment 1

[0032] The examples in this part are intended to illustrate the fermentation process of Mortierella sinensis in the present invention.

[0033] Firstly, the seeds were cultured in shake flasks to obtain the primary seed solution. Specifically, the Mycelium of Mortierella sinensis on the solid medium is scratched off with an inoculation loop, a blade and other tools, and inserted into a first-class seed shaker flask in a sterile environment for shaking culture on a shaking table. The rotating speed of the shaking table is 160rpm. The cultivation temperature is 20°C, the cultivation time is 24-28h, and finally the primary seed liquid is obtained.

[0034] Then carry out seed tank culture to obtain secondary seed liquid. The specific steps are: put all the primary seed liquid into the seed tank for seed liquid cultivation, cultivate at 20° C. for 16-22 hours, and obtain the secondary seed liquid. The culture volume of the first-level seed shake flask is much smaller than the cu...

Embodiment 2

[0042] The examples in this part are intended to illustrate the fermentation of Mortierella sinensis without adding any oxygen carrier.

[0043] In particular, refer to the fermentation operation method in Example 1, the shake flask culture medium, the seed tank culture medium and the fermentation medium are all the same as in Example 1, the fermentation temperature is 20°C, the inoculum size is 20%, and the glucose concentration in the tank is controlled at ≤0.5%. The difference is that no oxygen carrier is added.

[0044] Based on the fermentation cycle, cell yield, adenosine content, and specific oxygen consumption rate (Q O2 ), oxygen uptake rate (R), air oxygen critical concentration, and power consumption as indicators, the results are shown in Table 1:

[0045] In particular, the concept of indicators mentioned above is as follows:

[0046] Specific oxygen consumption rate (breathing intensity) (Q O2 ): refers to the amount of oxygen consumed per hour by the dry tha...

Embodiment 3

[0053] The examples in this part are intended to illustrate the fermentation of Mortierella sinensis when n-dodecane is added as an oxygen carrier.

[0054] In particular, refer to the fermentation operation method in Example 1, the shake flask culture medium, the seed tank culture medium and the fermentation medium are all the same as in Example 1, the fermentation temperature is 20°C, the inoculum size is 20%, and the glucose concentration in the tank is controlled at ≤0.5%. The difference is that n-dodecane is started to be fed in the middle stage of fermentation (16h) and fed until the end of fermentation.

[0055] Based on the fermentation cycle, cell yield, adenosine content, and specific oxygen consumption rate (Q O2 ), oxygen uptake rate (R), air oxygen critical concentration, power consumption, oxygen carrier recovery rate, oxygen carrier cycle times as indicators, the results are shown in Table 2:

[0056] In particular, the recovery rate of the oxygen carrier refe...

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Abstract

The invention discloses a method for improving the fermentation efficiency of hirsutella sinensis by using an oxygen carrier, which is characterized in that the oxygen carrier is used in the fermentation process, so that the oxygen utilization efficiency of thalli is improved, the yield of artificially cultured hirsutella sinensis thalli and the yield of substances such as cordycepin and adenosine are higher, the fermentation cost is reduced, and the emission of wastewater and waste gas is reduced. The oxygen carrier comprises substances such as n-dodecane, n-hexadecane, perfluorodecahydronaphthalene, olive oil and the like, and has the advantages of being small in dosage, convenient to obtain, capable of being recycled and reused, not absorbed and utilized by thalli and the like. The final thallus yield can reach up to 30.7%, the adenosine yield is 2.92 mg / g, the specific oxygen uptake rate (QO2) and the oxygen uptake rate (R) are obviously improved, the air oxygen critical concentration is obviously reduced, the power consumption is obviously reduced, the use of water and electricity is greatly reduced, the fermentation period is shorter, the efficiency is higher, and the product competitiveness of hirsutella sinensis produced by a fermentation method is improved.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for fermentation and cultivation of Trichosia chinensis, which is a liquid fermentation method for high-efficiency oxygen transfer of Trichosia chinensis, and the method utilizes an oxygen carrier to improve the fermentation efficiency of Trichosia chinensis . Background technique [0002] Cordyceps sinensis (C. sinensis (Berk.) Sacc.) of the fungi kingdom (Fungi) ergot family (Clvaieipiactae) Cordyceps (Cordyceps) is more common in mountainous shady and semi-shady plateau meadows and alpine irrigation. Among the clump vegetation, it is especially common in snow-covered and well-drained alpine meadows, mainly distributed in Tibet, Qinghai, Gansu, Sichuan, Yunnan and other places in my country. Cordyceps sinensis has become a rare and precious Chinese herbal medicine in my country due to its special growth mode and unique growth environment and its important medici...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 徐庆阳耿燕周帅王鹏赵伏梅朱正乔
Owner JIANGSU SHENHUA PHARMA
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