Special culture medium for hirsutella sinensis and preparation method thereof

Abstract

The invention discloses a special culture medium for hirsutella sinensis and a preparation method thereof, and belongs to the field of biotechnology. 1000 mL of the culture medium is mainly prepared from 400 mL of beef juice, 200 mL of fresh milk, 0.5 g of compound vitamin B, 1 g of dipotassium phosphate, 0.5 g of sodium chloride, 0.5 g of biotin, 5 g of lactoalbumin hydrolysate, 0.5 g of nucleic acid, 1 g of yeast powder, 16 g of agar and 400 mL of water. The culture medium disclosed by the invention has the advantages of short delay phase of the inoculated hirsutella sinensis, rapid growth, robust hypha, stable hypha character, stable yield and the like, and is a very mature and stable culture medium formula in the research of the hirsutella sinensis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a special medium for Mortierella chinensis and a preparation method thereof. Background technique [0002] Cordyceps sinensis, also known as Cordyceps sinensis, is one of the precious and nourishing Chinese medicinal materials. It is also known as the "three treasures" of Chinese traditional medicine together with velvet antler and ginseng. It is a complex of insects and bacteria formed after Cordyceps sinensis infects the larvae of bat moths. Cordyceps sinensis in the world is only distributed in alpine meadows at an altitude of 2800-5000m in four countries around the Qinghai-Tibet Plateau, including China, Nepal, Bhutan and India. However, the distribution of Cordyceps sinensis mentioned in some domestic articles is not correct in Shanxi, Henan, Taiwan and other countries or regions such as the United States and Japan, because other types of Cordyceps are mistaken for ...

AI Technical Summary

Problems solved by technology

Realizing the artificial cultivation of Cordyceps sinensis has always been the common dream of researchers of Cordyceps sinensis, and many researchers have made unremitting efforts for this. However, this dream has not been realized until now. The reason is that the physiological characteristics of Cordyceps sinensis itself complexity of

Existing research data show that Hirsutella sinensis is an anamorph of Cordyceps sinensis. In order to realize the full artificial cultivation of Cordyceps sinensis, it is necessary to carry out in-depth research on the characteristics of Hirsutella sinensis. However, Hirsutella sinensis Physiological characteristics are relatively complex, it cannot grow or grows very slowly on ordinary medium, and it is difficult to grow grass, which has become a bottleneck factor in its research