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Paecilomyces lilacinus fungal spore oil suspending agent and preparation method as well as application thereof

A technology of Paecilomyces lilacinus and fungal spores, which is applied in the field of pesticides, can solve limitations and other problems, and achieve the effects of complete emulsification stability, low application cost, and safe dispersants

Inactive Publication Date: 2012-10-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although domestic research on Paecilomyces lilacinus preparations has begun, it is still limited to some simple dosage forms, such as powders and granules, and new dosage forms have not been reported yet.

Method used

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  • Paecilomyces lilacinus fungal spore oil suspending agent and preparation method as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: the storage time determination of Paecilomyces lilacinus fungus spore oil suspension

[0041] Mix Paecilomyces lilacinus fungal spore powder, emulsifier (NP-4 and EL-20), thickener (xanthan gum), dispersant (YUS-EP60P) and vegetable oil (salad oil) according to the formula shown in the table below ) in a sand mill for 10 minutes to prepare Paecilomyces lilacinus fungus spore oil suspension; get 1ml of Paecilomyces lilacinus fungus spore oil suspension into a 1.5ml centrifuge tube, store at room temperature, The spore germination rate was determined weekly.

[0042] Table 1 Paecilomyces lilacinus fungus spore oil suspension formulation

[0043]

[0044] The experimental results show that the spore activity of Paecilomyces lilacinus fungus spore oil suspension is 80% after being stored for one month, and the shelf life of Paecilomyces lilacinus fungus spore oil suspension at room temperature is about 8 months through linear relationship calculation Comp...

Embodiment 2

[0045] Embodiment 2: Indoor nematicidal activity determination of Paecilomyces lilacinus spore oil suspension

[0046] (1) Preparation of Paecilomyces lilacinus fungus spore oil suspension

[0047] Paecilomyces lilacinus is a nematode-eating fungus that infects nematodes through spore germination and hyphae growth. Under the condition that the ambient temperature is not higher than 30°C, 72wt% of methylated soybean oil, 7wt% of EL-20, 4wt% of NP-4, 4wt% of AEO-3, 3wt% of YUS-CH1100, 5 wt% Vaseline and 5 wt% Paecilomyces lilacinus fungus spore powder were added to a sand mill and ground at a speed of 1000-1500r / min for 10 minutes to make Paecilomyces lilacinus fungus spore oil suspension ; Paecilomyces lilacinus fungus spore oil suspension was diluted 1 × 10 5 , 1×10 7 , 1×10 9 Times, made into a suspension, used for indoor nematicidal activity determination;

[0048] (2) Preparation of nematode suspension

[0049] Pick the ovum mass of root-knot nematode incognita from liv...

Embodiment 3

[0054] Under the condition that the ambient temperature does not exceed 30°C, 10wt% of Paecilomyces lilacinus fungus spore powder, 8wt% of EL-20, 7wt% of AEO-3, 5wt% of EL-12, 5wt% of YUS- CH1100, 5wt% petrolatum and 60wt% soybean oil were added to a blender and stirred for 30 minutes to prepare 10wt% Paecilomyces lilacinus fungus spore oil suspension. When in use, dilute 2000-5000 times with water, and apply evenly in the way of furrow application or hole application in combination with soil preparation before sowing.

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Abstract

The invention discloses a paecilomyces lilacinus fungal spore oil suspending agent and a preparation method as well as application thereof, belonging to the technical field of pesticides. The suspending agent is prepared from the following components in percentage by weight: 1-20 percent of paecilomyces lilacinus fungal spore powder, 10-30 percent of emulsifier, 2-10 percent of thickening agent, 3-15 percent of dispersant and 25-84 percent of plant oil. The preparation method comprises the following step of: stirring the various components weighed according to the proportion in a stirring machine under the condition of environmental temperature of not higher than 30 DEG C for 0.5-1 hour, grinding the various components weighed according to the proportion in a sand mill at the rotation speed of 1,000-15,000 r / min for 10-20 minutes or shearing the various components weighed according to the proportion in a high shear at the rotation speed of 10,000-50,000 r / min for 5-10 minutes to obtain the paecilomyces lilacinus fungal spore oil suspending agent. The suspending agent has the advantages of obvious prevention and treatment effect on nematode, long duration of pesticide effect, environmental friendliness, safety and capability of promoting plant growth.

Description

technical field [0001] The invention belongs to the technical field of pesticides, and in particular relates to a Paecilomyces lilacinus fungus spore oil suspension and a preparation method and application thereof. Background technique [0002] Paecilomyces lilacinus is a biological agent, which has the characteristics of high efficiency, safety and long-lasting effect in the process of killing nematodes. In addition, Paecilomyces lilacinus can also promote the growth of plants and achieve the purpose of increasing production. The existing formulations of Paecilomyces lilacinus include powder and wettable powder. Various dosage forms have their own advantages and disadvantages. Powder is the earliest dosage form developed and used by fungi, and its preparation method is simple; however, spore powder has disadvantages such as short storage time, inconvenient use, uneven distribution, and slow efficacy. Compared with powder, wettable powder is convenient to use, and it can be...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/04A01P3/00A01G13/00
Inventor 杜凤沛殷丽
Owner CHINA AGRI UNIV
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