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Entomopathogenic Fungi and Uses Thereof ?

A technology for fungi and uses, applied in the field of entomopathogenic fungi and their metabolites, can solve the problems of prosperous, non-native fungi that are not suitable for survival or other problems

Inactive Publication Date: 2012-02-01
BIOTELLIGA HLDG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, many non-native fungi may not be suitable or capable of surviving or thriving in local conditions

Method used

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  • Entomopathogenic Fungi and Uses Thereof ?

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0209] Identification and isolation of embodiment 1 Beauveria bassiana bacterial strain K4B3

[0210] Beauveria bassiana K4B3 was first isolated from a colony of dead cicada pupae that had reached the soil surface and died in large numbers in pine forests in Bombay, New Zealand. Fungi were isolated from insect samples using standard procedures, which included growth at 24 °C and 93% relative humidity to maximize sporulation. Single colonies were then subcultured on MEA to generate pure strains for screening insect pathogenic potency.

[0211] Beauveria bassiana characteristics

[0212] Using taxonomic criteria well known in the art, the isolate was identified as Beauveria bassiana.

[0213] Morphological features

[0214] Isolate K4B3 is pathogenic to juvenile thrips, adult thrips and thrips pupae, aphids and whiteflies. This isolate has the following identifying characteristics:

[0215] Mycelium: Easy to grow on MEA. Colonies are usually white, turning cream to pale ye...

Embodiment 2

[0220] Example 2 - Comparison of Whitefly Control Using K4B3 and Chemical Insecticides

[0221] introduction

[0222] This example describes field trials conducted to evaluate the efficacy of Beauveria bassiana strain K4B3 as a biological control agent for whiteflies and compares it to established chemical treatment programs. Tested at 2 1680m 2 It was performed in a Venlo-type Faber greenhouse with coal-fired evaporators and Chemtest environmental and irrigation controllers. The greenhouse is identical in all respects except for the runoff runoff. The chemical pesticide program was carried out in Greenhouse 1 and the BCA test of the present invention was carried out in Greenhouse 2.

[0223] method

[0224] Greenhouse 1

[0225] The De Ruiter variety Antarctica is grown in this greenhouse. As is usual after establishment, allow plants to reach knee height, then pass 100ml / 1000m 2 For irrigation, apply Vydate (240gm / L oxamyl). Increase the dose of Vydate to 200ml / 100m...

Embodiment 3

[0234] The preparation of embodiment 3-beauveria bassiana

[0235] introduction

[0236] This example describes the large-scale solid phase growth of Beauveria bassiana strain K4B3 and the preparation of compositions comprising one or more metabolites thereof.

[0237] method

[0238] Germination of conidia

[0239] Optimal sporulation requires a saturated atmosphere and a temperature of 25 to 30°C. After sporulation, the spores were transferred to dry sealable containers and stored at 8°C. Spores can be stored this way for up to 635 days.

[0240] solid phase, large scale growth

[0241] After 300 days of storage, the hydrophobic spore powder was removed from storage and tested for viability as follows.

[0242] Malt extract agar was prepared at pH 5.5 (supplemented with 20,000 I.U. penicillin / L and 40 mg streptomycin / L). Add a 1 mL aliquot of the spore suspension to malt extract agar, spread and incubate at 24 °C for 14 days.

[0243] On day 15, the fungi were harve...

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Abstract

The present invention provides a strain of entomopathogenic Beauveria bassiana, compositions comprising the entomopathogenic fungi strain or metabolites of the strain, and the use of the entomopathogenic fungi strain and compositions as biological control agents. Methods for the biological control of phytopathogenic insects using an entomopathogenic Beauveria bassiana fungi strain or one or more metabolites thereof, optionally together with other entomopathogenic fungi including fungi selected from strains of Lecanicillium spp., Paecilomyces fumosoroseus, and compositions comprising said fungi or metabolites thereof are also provided.

Description

field of invention [0001] The present invention relates to entomopathogenic fungi and their metabolites, compositions comprising said entomopathogenic fungi or one or more of their metabolites and the use of these entomopathogenic fungi and compositions as biological control agents. Also provided is the use of the entomopathogenic fungus Beauveria bassiana (Beauveria bassiana) and compositions comprising said fungus or one or more of its metabolites for phytopathogenic insects including aphids, thrips, whiteflies, mealybugs, etc. and other methods of biological control. Background of the invention [0002] Plant diseases caused by pathogens such as insects are an important economic cost of plant-based agriculture and industry. Losses can be caused by damage to the product before and after harvest, loss of the plant itself, or by a reduction in growth and productivity. [0003] Traditionally, control of plant pathogens has been sought through the application of chemical pes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01N63/30
CPCC12R1/645A01N63/04C12N1/14A01N63/30C12R2001/645C12N1/145A01N25/00C12N11/00
Inventor S·R·福特
Owner BIOTELLIGA HLDG
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