The invention discloses probiotics-containing chicken feed and a preparation method of the probiotics-containing chicken feed. The probiotics-containing chicken feed is prepared from the following raw materials in parts by weight: 25 to 35 parts of wheat, 15 to 25 parts of corn distiller dried grains, 7 to 10 parts of soybean meal, 7 to 9 parts of cottonseed meal, 10 to 15 parts of vegetable oil, 4 to 7 parts of maifanite powder, 4 to 6 parts of fish meal, 3 to 5 parts of folium artemisiae argyi powder, 0.2 to 0.5 part of calcium hydrogen phosphate, 0.5 to 0.8 part of salt, 0.1 to 0.3 part of lysine, 0.1 to 0.3 part of methionine, 0.2 to 0.4 part of probiotics, 0.5 to 0.8 part of premix compound, 0.8 to 1.2 parts of radix astragali, 0.5 to 1 part of radix glycyrrhizae, 0.5 to 1 part of radix angelica sinensis, 0.9 to 1.5 parts of radix bupleuri, 0.5 to 1.4 parts of citri reticulatae pericarpium and 0.4 to 1 part of fructus ligustri lucidi. According to the probiotics-containing chicken feed disclosed by the invention, the probiotics is selected as one part of an additive for chickens, so that the food digestion of the chickens can be promoted, pathogenic bacteria in the intestinal canal are inhibited, the growth and the propagation of beneficial bacteria in the intestinal canal are promoted, and the functions of invigorating spleen and replenishing qi, promoting digestion and relieving food retention, enhancing the immunity, and the like are obtained.