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Microbial agent for promoting aerobic composting of dead pigs and application of microbial agent

A technology of microbial agent and dead pigs, which is applied in the direction of microorganisms, microorganisms, and animal carcass fertilizers, can solve the problems of less treatment of animal carcasses infected with epidemics, failure to promote them, and long fermentation cycle, so as to speed up carcass decomposition, facilitate promotion and Easy application and preparation

Active Publication Date: 2018-02-02
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the microbial agents on the market for harmless treatment and resource utilization are mainly concentrated in livestock and poultry manure, crop straw, etc., while there are few reports on the treatment of infected animal carcasses, and the fermentation period is long and the degradation is not complete. The impact cannot be generalized
How to solve the problems of long fermentation cycle and incomplete degradation, which plague the development of pig carcass bacterial agents

Method used

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  • Microbial agent for promoting aerobic composting of dead pigs and application of microbial agent
  • Microbial agent for promoting aerobic composting of dead pigs and application of microbial agent
  • Microbial agent for promoting aerobic composting of dead pigs and application of microbial agent

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Experimental program
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Effect test

Embodiment 1

[0035] Isolation and identification of Bacillus licheniformis ZR-1:

[0036] The applicant isolated, screened, and identified a strain of Bacillus licheniformis from natural compost samples of dead pig corpses. The strain was sent to the China Collection Center for Type Cultures on June 26, 2017. The taxonomic name: Bacillus licheniformis licheniformis ZR-1, the deposit number is: CCTCC NO: M2017384.

[0037] The 16S rDNA gene sequence of the Bacillus licheniformis ZR-1 bacterial strain is compared with the sequence in GenBank, and it is found that the homology of the 16S rDNA sequence of the bacterial strain ZR-1 isolated by the present invention and the known sequence reaches 99%, after identification, it is determined that the The strain is Bacillus licheniformis, and the strain has been sent to the China Center for Type Culture Collection on June 26, 2017 for preservation. The classification name is Bacillus licheniformis ZR-1, and the preservation number is: CCTCC NO: M20...

Embodiment 2

[0041]Detection of enzyme production ability of Bacillus licheniformis ZR-1:

[0042] Lipase assay:

[0043] After putting Bacillus licheniformis ZR-1 in the oily liquid medium for 24h, take 1mL of the bacteria solution (OD 600 =1) add to the Erlenmeyer flask respectively. Another Erlenmeyer flask was used as a control. Add 4 mL of substrate solution and 5 mL of phosphate buffered saline to all Erlenmeyer flasks, respectively. Immediately mix the timing, water bath 10min. Then 15 mL of 95% ethanol was added to all Erlenmeyer flasks to stop the reaction. Add 3-5 drops of phenolphthalein reagent to all Erlenmeyer flasks, and titrate with 0.05mol / L NaOH standard solution.

[0044] Calculation result, lipase enzyme activity unit is defined as: under certain conditions, the enzyme amount that releases 1umol fatty acid per minute is defined as 1 lipase activity unit (U). Calculate the enzyme activity by the following formula: X=(B-A)×5, where: X is the enzyme activity (U / mL) o...

Embodiment 3

[0061] A microbial agent for promoting aerobic composting of dead pig corpses, the preparation method comprising the following steps:

[0062] Preparation of Bacillus licheniformis, Bacillus subtilis, methylotrophic Bacillus, Bacillus amyloliquefaciens seed liquid:

[0063] Seed medium: beef extract peptone medium (beef extract 3.00g / L, peptone 10.00g / L, NaCl 5.00g / L, pH 7.4-7.6). The culture condition is 37° C., the rotation speed is 220 rpm, and the culture is 12-16 hours.

[0064] Preparation of microbial agents: Bacillus licheniformis, Bacillus subtilis, methylotrophic Bacillus, and Bacillus amyloliquefaciens were cultivated until the cell concentration was OD 600 The seed solution of =1 is mixed and fermented according to the volume ratio of 3:3:2:1, with the fermentation medium as the mixed bacteria culture medium fermentation medium, and the filling amount is 60%, adding food-grade defoamer vegetable oil and foam enemy. The operation steps are as follows: sterilize th...

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Abstract

The invention discloses a microbial agent for promoting aerobic composting of dead pigs and an application of the microbial agent. The microbial agent includes bacillus licheniformis ZR-1, bacillus subtilis dcy-1, bacillus methylotrophicus F7 and bacillus amyloliquefaciens YZ-0001. The microbial agent beneficial to degradation of organic matter is inoculated in the beginning of composting, maximumtemperature of a composting body is increased, high-temperature phase of the composting body is prolonged, pathogenic microbes are inactivated, composting of dead bodies is fastened, degradation of plant toxicity is promoted, and seed germination index is increased; the microbial agent is low in cost, simple in composition, convenient in preparation and easy to promote and apply; the microbial agent of aerobic composting of the dead pigs can generate stable humus and can be used as organic fertilizer applied to crop planting, suppress phytopathogens and reduce use of the fertilizer, and gooddevelopment prospect is achieved.

Description

technical field [0001] The invention belongs to the technical field of harmless treatment of sick and dead livestock and poultry, and in particular relates to a microbial agent for promoting aerobic composting of dead pig corpses and its application. Background technique [0002] While modern intensive farming improves the overall productivity, it also produces a large amount of pollutants such as livestock manure, sewage, and dead livestock and poultry residues. According to statistics, in 2016, the number of pigs slaughtered in my country was about 680 million. Under normal circumstances, the mortality rate of pigs caused by various diseases in my country is about 8% to 12%. A large number of animal carcasses, these dead pigs only carry a large number of pathogenic microorganisms, if not handled properly, it will cause the spread of pathogenic bacteria, cause major epidemics, and seriously threaten the ecological environment and human public health safety. At present, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/10C12R1/125C12R1/07
CPCC05F1/00C05F17/00C12N1/20C12N1/205C12R2001/10C05F11/08Y02W30/40
Inventor 郑嵘户青青杨旭晨王红帅蒋思文柴进
Owner HUAZHONG AGRI UNIV
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