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Application of pseudoxanthomonas composti and microbial preparation thereof in biological composting

A technology for pseudoxanthomonas and microbial preparations is applied in the field of environmental microorganisms to achieve the effects of shortening the compost fermentation cycle, increasing the fermentation temperature, and improving the quality and efficiency of compost fermentation

Active Publication Date: 2019-02-01
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report about the application of Pseudoxanthomonas composti and its microbial preparations in biocomposting

Method used

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  • Application of pseudoxanthomonas composti and microbial preparation thereof in biological composting
  • Application of pseudoxanthomonas composti and microbial preparation thereof in biological composting
  • Application of pseudoxanthomonas composti and microbial preparation thereof in biological composting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1P

[0027] Example 1P.composti GSS15 T Cultivation, isolation, screening, identification and preservation of

[0028] 1.1 Culture and isolation of strains

[0029] P. composti GSS15 of the present invention T It is obtained from the enrichment culture of urban domestic sludge compost samples for separation and purification.

[0030] Concrete separation and purification method comprises the following steps:

[0031] (1) Take 5.0g of urban domestic sludge compost samples and inoculate them in a collection and separation medium with a pH value of 7.2, and cultivate them with shaking at 30°C and 200rpm / min; after cultivating for 3 days, transfer the culture solution to Connect to another fresh collection separation medium, shake culture at 30°C and 200rpm / min. Such enrichment culture for four generations.

[0032] (2) The culture solution after the fourth generation reaction was diluted to 10 by the dilution plate method. -5 -10 -6 , spread 0.1 mL of the diluted culture solutio...

Embodiment 2P

[0047] Example 2P.composti GSS15 T Preparation of Microbial Preparations

[0048] The specific method is:

[0049] (1) Shake flask culture: from P.composti GSS15 T Pick a ring of bacterial lawn on the inclined surface and put it into the Erlenmeyer flask filled with LB liquid medium, and cultivate it at 37°C and 200rpm / min on a shaking table for 24h. The formula of the LB liquid medium is (g / L): 5 g of yeast extract; 10 g of tryptone; 10 g of NaCl; 1000 mL of water; pH 7.2.

[0050] (2) Seed tank fermentation: the P.composti GSS15 cultured above T The fermentation broth was put into a 10L fermenter, and fermented at 37° C. and 180 rpm / min for 36 hours; the formula of the fermentation medium used was the above-mentioned liquid medium.

[0051] (3) fermenter fermentation: the above-mentioned cultured P.composti GSS15 T The fermentation liquid was put into a fermenter, and fermented at 37° C. and 200 rpm / min for 48 hours; the formula of the fermentation medium used was the a...

Embodiment 3P

[0053] Example 3P.composti GSS15 T Preparation of Microbial Preparations

[0054] The specific method is:

[0055] (1) Shake flask culture: from P.composti GSS15 T Pick a ring of bacterial lawn on the inclined surface and put it into the Erlenmeyer flask filled with LB liquid medium, and culture it on a shaker at 30°C and 200rpm / min for 24h. The formula of the LB liquid medium is (g / L): 5 g of yeast extract; 10 g of tryptone; 20 g of NaCl; 1000 mL of water; pH 7.2.

[0056] (2) Seed tank fermentation: the P.composti GSS15 cultured above T The fermentation broth was put into a 10L fermenter, and fermented at 30° C. and 200 rpm / min for 24 hours; the formula of the fermentation medium used was the above-mentioned liquid medium.

[0057] (3) fermenter fermentation: the above-mentioned cultured P.composti GSS15 T The fermentation liquid was put into a fermenter, and fermented at 30°C and 200 rpm / min for 48 hours; the formula of the fermentation medium used was the above-mentio...

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Abstract

The invention discloses an application of pseudoxanthomonas composti and a microbial preparation thereof in biological composting. The pseudoxanthomonas composti GSS15T is classified as pseudoxanthomonas composti GSS15T, is preserved in Guangdong Microbial Culture Collection Center, and has the preservation number of GDMCC No.60454. According to the microbial germplasm resource GSS15T and the microbial preparation thereof, the microbial preparation prepared by the pseudoxanthomonas composti GSS15T can improve the fermentation temperature of organic waste high-temperature aerobic composting, shortens the composting fermentation period, and improves the quality and efficiency of composting fermentation.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to the application of a strain of pseudoxanthomonas and microbial preparations thereof in biocomposting. Background technique [0002] The amount of agricultural waste in our country is large and wide, and the environmental pollution is serious. Agricultural organic waste mainly includes livestock and poultry manure and crop straw, among which livestock and poultry manure is more prominent and has become an important source of environmental non-point source pollution. Agricultural waste is a major source of environmental pollution, and it is also a huge biomass resource and energy pool. For example, livestock and poultry manure contains a large amount of nutrients such as nitrogen, phosphorus, and potassium necessary for the growth of crops and a large amount of organic matter, which can be used to produce high-quality organic waste. Fertilizer, increase soil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/01
CPCC05F3/00C05F17/00C12N1/20C12N1/205C12R2001/01C05F11/00C05F11/08Y02W30/40
Inventor 王跃强余震汤佳刘晓明周顺桂周普雄
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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