Special seed coat eco-fertilizer for planting peanuts in red soil and preparation method and application thereof
An ecological fertilizer and peanut technology, applied in the agricultural field, can solve the problems of declining economic benefits, slow growth of peanuts, and destruction of ecological balance, and achieve the effects of enhancing vitality and nodule formation, slowing root growth, and promoting nitrogen fixation activity
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Embodiment 1
[0021] (1) Bacteria production:
[0022] 1) Peanut Rhizobium: The composition of the liquid medium is 0.08-0.2% (weight / volume) of yeast extract, 0.5-1.5% (volume / volume) of mannitol, 0.05-0.2% magnesium sulfate (weight / volume), 0.1-0.4 % Iron trichloride (weight / volume), 0.05-0.2% sodium molybdate (weight / volume), 0.01-0.1% zinc sulfate (weight / volume), 0.05-0.15% potassium dihydrogen phosphate (weight / volume), 0.08-0.2% calcium chloride (weight / volume), pH 6.6-7.6, sterilized at 121°C for 25-40 minutes. Cool to about 35℃, inoculate peanut rhizobia, the inoculation amount is about 10 per ml of liquid medium 5 -10 6 One peanut rhizobia cultured on a shaker at a speed of 100-180 revolutions per minute. After 48-72 hours, sample the fermentation broth and check that the number of rhizobia reached 20x10 per ml of liquid. 8 At this time, the fermentation is over;
[0023] 2) Bacillus subtilis: The composition of the liquid medium is peptone 0.08-0.1% (weight / volume), beef extract 10-3...
Embodiment 2
[0034] (1) Bacteria production:
[0035] 1) Peanut rhizobia liquid 1000mL: The liquid culture medium consists of 1g yeast extract, 1.2g mannitol, 1g magnesium sulfate, 2g ferric chloride, 2g sodium molybdate, 0.5g zinc sulfate, 1g potassium dihydrogen phosphate, and chlorinated Calcium 1g, pH 6.6-7.6, sterilized at 121°C for 25-40 minutes. Cool to about 35℃, inoculate peanut rhizobia, the inoculation amount is about 10 per ml of liquid medium 5 -10 6 One peanut rhizobia cultured on a shaker at a speed of 100-180 revolutions per minute. After 48-72 hours, sample the fermentation broth and check that the number of rhizobia reached 20x10 per ml of liquid. 8 When the fermentation is over, 1000 mL of peanut rhizobia fermentation broth is obtained;
[0036] 2) Bacillus subtilis 1000mL: The liquid medium is composed of 1g peptone, 20g beef extract, 6g sodium chloride, 1g magnesium sulfate, 2g ferrous sulfate, 1g potassium dihydrogen phosphate, 1g calcium chloride, pH 6.6-7.6, 121 Sterili...
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