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36 results about "Paecilomyces spectabilis" patented technology

Paecilomyces variotii is a common environmental mold that is widespread in composts, soils and food products. It is known from substrates including food, indoor air, wood, soil and carpet dust. Paecilomyces variotii is the asexual state of Byssochlamys spectabilis, a member of the Phylum Ascomycota (Family Trichocomaceae).

Bacillus subtilis Pc3 and use of bacillus subtilis Pc3 in preparation of fermentation supernatant for preventing and controlling plant pathogenic fungi

The invention relates to bacillus subtilis Pc3 and a use of bacillus subtilis Pc3 in preparation of a fermentation supernatant for preventing and controlling plant pathogenic fungi, and relates to bacilli. The bacillus subtilis Pc3 has a preservation number of CCTCC NO: M2013470. The bacillus subtilis Pc3 can prevent and control plant pathogenic fungi such as gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani and fusaria. A rape sclerotinia sclerotiorum prevention and control pot experiment proves that the bacillus subtilis Pc3 has good biological-prevention and control effects and has prevention and control efficiency of 95.6%. The fermentation supernatant of the bacillus subtilis Pc3 can be prepared by simple processes and has a wide fungus inhibition spectrum and good biological prevention effects.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION

Paecilomyces variotii bacterial strain SJ1 and application thereof

The invention discloses Paecilomyces variotii bacterial strain SJ1 and in particular relates to sea-buckthorn fungus, a preparation of sea-buckthorn endophytic fungi and extract of the sea-buckthorn fungus and use of the sea-buckthorn fungus extract. By utilizing the Paecilomyces variotii bacterial strain SJ1, growth and root development of crop, such as corn, rice are promoted, antifreezing capacity is improved, and yield is increased. The SJ1 bacterial strain is Paecilomyces variotii according to morphological and genetic analysis and identification.
Owner:山东蓬勃生物科技有限公司

Bacillus DY26-004 and application thereof to prevention and control of plant pathogenic fungi

The invention relates to bacilli, in particular to a bacillus DY26-004 and application thereof to prevention and control of plant pathogenic fungi. A preservation number of the Bacillus sp.DY26-004 is CCTCC NO:M 2015406. The bacillus DY26-004 has a strong inhibiting effect on various plant pathogenic fungi such as fusaria, colletotrichum musae, paecilomyces variotii, sclerotinia scleotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani, alternaria alternata, botrytis cinerea and colletotrichum gloeosporioides, and has good biological prevention and control effects in pot experiments for prevention and control of sclerotinia scleotiorum and fusarium graminearum, wherein control efficiencies are 72% and 65% respectively. The bacillus DY26-004 which is a nontoxic, non-pathogenic and environment-friendly bacillus is a useful resource for research and development of microbial pesticides and has a potential application prospect.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION +1

One-strain high-ring polycyclic aromatic hydrocarbon degradation bacterium and uses thereof

The invention discloses epipodium polycyclic aromatic hydrocarbons degrading bacteria and the application thereof. The epipodium polycyclic aromatic hydrocarbons degrading bacteria (paecilomyces variotii) F6 provided by the invention has the function of pipodium polycyclic aromatic hydrocarbons and pipodium polycyclic aromatic hydrocarbons mixture. After the pipodium polycyclic aromatic hydrocarbons is cultured for 30 d in a polycyclic aromatic hydrocarbons compound system, the degrading rate to the polycyclic aromatic hydrocarbons becomes 16.06 percent to 24.57 percent; however, the degrading rate in a single polycyclic aromatic hydrocarbons compound system becomes 10.42 percent to 33.25 percent, wherein, the degrading rate of benzo (k) fluoranthene and benzo (b) fluoranthene is the highest, which respectively accounts for 33.25 percent and 26.44 percent. The strain provided by the invention can supply novel germplasm resource for developing the co-metabolism mechanism study of a plurality of epipodium polycyclic aromatic hydrocarbons and the construction of aromatic hydrocarbons combined pollution water-soil environment repairing technology.
Owner:SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP

Broad-spectrum fungus-resistant bacillus and application thereof to preventing wheat scab

The invention discloses broad-spectrum fungus-resistant bacillus and application thereof to preventing wheat scab and relates to bacillus. The preservation number of the bacillus (Bacillus sp.)DY26-010 is CCTCC NO:M2015407. Supernatant liquid of the bacillus is antagonistic to fusarium spp, gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia, mutualism alternaria, botrytis cinerea and colletotrichum dematium. Fermentative supernatant liquid of the bacillus DY26-010 has high antagonism on pathogenic bacteria and fusarium spp of wheat scab, the preventing effect can be 57.1% in a potted experiment of wheat scab, and the bacillus can be used for preparing the fermentative supernatant liquid applied to preventing wheat scab. A biological prevention method of wheat scab is provided, no chemical pesticide is used, the bacillus has the advantages of being non-toxic, not prone to forming a pesticide-resistant environment, biologically safe and the like, and application prospects are good.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION +1

Antarctic-derived Bacillus sp.N311 and application of Bacillus sp.N311 for preventing and treating phytopathogenic fungi

The invention relates to Antarctic-derived Bacillus sp.N311 and an application of Bacillus sp.N311 for preventing and treating phytopathogenic fungi, which relate to Bacillus sp. A preservation number of the Bacillus sp.N311 is CCTCC NO: M 2015607. The Bacillus sp.N311 can be used for preparing a fermentation supernatant for preventing and treating phytopathogenic fungi. The phytopathogenic fungi comprise fusarium, gloeosporium musarum, Paecilomyces variotii, sclerotinia sclerotiorum, Alternaria longipes, Trichoderma viride, Rhizoctonia solani, alternaria alternate, botrytis cinerea and Colletotrichum gloeosporioides. The Bacillus sp.N311 has good biological prevention and treatment effects in Rape Sclerotinia Rot pot culture prevention and treatment tests, and prevention and treatment effects can reach more than 50%. The Bacillus sp.N311 fermentation supernatant has wide fungus-inhibiting spectrum and good biocontrol effect, has advantages of no toxicity, difficult generation of drug resistance, environment safety and biosafety, and has wide application prospect.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION

Production process for fermenting organic fertilizer by using Maotai-flavor liquor vinasse

The invention belongs to the technical field of chemical production, and relates to a production process for fermenting an organic fertilizer by using Maotai-flavor liquor vinasse. The production process comprises the following steps: (1) preparing a fermentation inoculant; (2) adding the fermentation inoculant in the step (1) into vinasse and auxiliary materials to prepare a fermentation raw material; and (3) transporting the fermentation raw materials obtained in the step (2) to a fermentation zone, and carrying out compost fermentation to obtain the organic fertilizer, the vinasse being Maotai-flavor liquor vinasse, and the fermentation inoculant being composed of Candida rugosa, Bacillus licheniformis, Paecilomyces variotii and Aspergillus fumigatus. According to the production process, the organic fertilizer production is performed on the Maotai-flavor Baijiu vinasse with characteristics of high acidity, high viscosity and degradation resistance under the conditions of natural pH,natural moisture and no additional chemical material adding, and the obtained organic fertilizer meets the national organic fertilizer standard (NY525-2012) and can be used for more scientifically and more effectively recycling and harmlessly utilizing the spent grains after the production of the Maotai-flavor liquor is finished, and has a wide application prospect in the Maotai-flavor liquor brewing industry.
Owner:KWEICHOW MOUTAI COMPANY

Paecilomyces variotii strain and application thereof

The invention relates to the technical field of biological strains, particularly a Paecilomyces variotii strain and application thereof. The Paecilomyces variotii strain MM2 is collected to the China General Microbiological Culture Collection Center on July 8th, 2013, and the collection number is CGMCC No.7903. The application method comprises the following steps: adding the strain into any of straw-bran liquid culture medium, filter paper-inorganic salt liquid culture medium or PDA (potato dextrose agar) liquid culture medium, and culturing at 28-30 DEG C for 2-9 days to obtain the single-strain solid microbial inoculant. The Paecilomyces variotii strain can efficiently degrade and utilize cellulose, can be used as an external strain source for recycling lignocellulose solid waste, and can accelerate the degradation of the cellulose; the Paecilomyces variotii strain is separated from wine sediment compost and applied to wine sediment compost, thereby avoiding the problem of difficulty in assessment; and after being added, the microbial inoculant containing the strain effectively promotes the rotting of the wine sediment compost and accelerates the processing of the organic fertilizer.
Owner:KWEICHOW MOUTAI COMPANY

Penicillium composition for promoting plant growth, fungicide and application thereof

The invention belongs to the technical field of microorganisms, and particularly relates to a penicillium composition for promoting plant growth, a fungicide and application of the penicillium composition. The invention provides a penicillium composition for promoting plant growth. The penicillium composition comprises Paecilomyces variotii, Penicillium digitatum, Penicillium islanneum and Penicillium purpureum. The penicillium composition can improve the fresh weight, dry weight, leaf number, plant height, chlorophyll content and hormone content of plants, can effectively promote plant growth, is high in biological safety and free of pollution, and can provide a theoretical basis for production of bio-organic fertilizers in the future.
Owner:YUNNAN UNIV

Antifungal protein and usage thereof

The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.
Owner:FOOD IND RES & DEV INST

Pholiota adiposa strain YX1 as well as culture method and application thereof

The invention discloses pholiota adiposa YX1 as well as a culture method and application thereof and belongs to the technical field of edible mushrooms. The pholiota adiposa YX1 has been preserved in the China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on December 1, 2020, and the preservation number of the YX1 is CGMCC NO. 21077. According to the pholiota adiposa YX1 as well as the culture method and application thereof, paecilomyces variotii fermentation broth obtained by separating endophytic fungi is used as one of raw materials of a liquid culture medium, so that not only is the mycelium biomass of the pholiota adiposa YX1 increased, but also the cost of the raw materials is reduced, and a technical support is provided for large-scale production. Meanwhile, fermentation liquor of the endophytic fungi is fully utilized, the growth promoting effect on other plants can be achieved, other reagents are not introduced, and ecological environment protection is facilitated.
Owner:山东蓬勃生物科技有限公司 +1

Method for detection of paecilomyces variotii

Disclosed is a method for detecting Paecilomyces variotii. In the method, Paecilomyces variotii is identified by using a nucleic acid represented by any one of the following nucleotide sequences (a) and (b): (a) a partial nucleotide sequence of ss-tubulin gene, which is depicted in any one of SEQ ID NO:1 to SEQ ID NO:4 and SEQ ID NO:22 to SEQ ID NO:33, or a sequence complementary to the partial nucleotide sequence; and (b) a nucleotide sequence which has the deletion, substitution or addition of one or several nucleotides in a nucleotide sequence depicted in any one of SEQ ID NO:1 to SEQ ID NO:4 and SEQ ID NO:22 to SEQ ID NO:33 and can be used for the detection of Paecilomyces variotii, or a sequence complementary to the nucleotide sequence.
Owner:KAO CORP

Penicillium composition with low-temperature protection effect, low-temperature protective agent, application of low-temperature protective agent and method for improving survival rate of floating seedlings

The invention belongs to the technical field of agricultural production and biology, and particularly relates to a penicillium composition with a low-temperature protective effect, a low-temperature protective agent, application of the penicillium composition and the low-temperature protective agent and a method for improving the survival rate of floating seedlings. The invention provides a penicillium composition with a low-temperature protection effect. The penicillium composition comprises penicillium citrinum, penicillium rubrum, penicillium torusii and paecilomyces variotii. The penicillium composition can effectively improve the survival rate and the cold resistance of floating breeding, has the advantages of being low in cost and easy to operate, and has the advantages of being high in safety and free of pollution due to the fact that microorganisms are adopted.
Owner:YUNNAN UNIV

A kind of Amanita liposa strain yx1, culture method and application thereof

The invention discloses a strain of Agaricus polyphagoides ( Pholiota Adiposa ) YX1, cultivation method and its application belong to the technical field of edible fungus. The lipopolyphaga YX1 has been deposited in the General Microorganism Center of China Microbiological Culture Collection Management Committee on December 1, 2020, and the strain preservation number is CGMCC NO.21077. The present invention adopts the Paecilomyces variotii fermentation liquid fermented liquid that endophytic fungus isolates to obtain as one of the raw materials of liquid culture medium, not only has increased Agaricum multiliposa ( Pholiota adiposa ) YX1 mycelium biomass, and reduce the cost of raw materials, to provide technical support for large-scale production. At the same time, the fermentation liquid of endophytic fungi is also fully utilized, which can promote the growth of other plants, without introducing other reagents, which is beneficial to the protection of the ecological environment.
Owner:SHANDONG PENGBO BIOTECHNOLOGY CO LTD +1

A kind of Paecilomyces variotii strain and application thereof

The invention relates to the technical field of biological strains, particularly a Paecilomyces variotii strain and application thereof. The Paecilomyces variotii strain MM2 is collected to the China General Microbiological Culture Collection Center on July 8th, 2013, and the collection number is CGMCC No.7903. The application method comprises the following steps: adding the strain into any of straw-bran liquid culture medium, filter paper-inorganic salt liquid culture medium or PDA (potato dextrose agar) liquid culture medium, and culturing at 28-30 DEG C for 2-9 days to obtain the single-strain solid microbial inoculant. The Paecilomyces variotii strain can efficiently degrade and utilize cellulose, can be used as an external strain source for recycling lignocellulose solid waste, and can accelerate the degradation of the cellulose; the Paecilomyces variotii strain is separated from wine sediment compost and applied to wine sediment compost, thereby avoiding the problem of difficulty in assessment; and after being added, the microbial inoculant containing the strain effectively promotes the rotting of the wine sediment compost and accelerates the processing of the organic fertilizer.
Owner:KWEICHOW MOUTAI COMPANY

Method for treating pH of nisin wastewater

The invention discloses a method for treating the pH of nisin wastewater, which comprises the steps of treating nisin wastewater with a fermentation method, applying Paecilomyces variotii to the treatment of nisin wastewater, cultivating it in the stock solution of nisin wastewater to maintain its vitality, aeration and stirring for 7~ In 9 days, the pH of the nisin wastewater can be neutralized and the COD can be reduced by 60% to 90%. The method for treating the pH of nisin wastewater obtained by the present invention only adds bacteria species and does not add other substances, and is very economical; moreover, the method is simple and easy to handle on a large scale.
Owner:TIANJIN AGRICULTURE COLLEGE

Liubao tea automatic stacking fermentation process and fermentation strain composition

The invention discloses a Liupao tea automatic pile fermentation strain composition which comprises a base bacterium solution, a consolidation bacterium solution and an aging bacterium solution. The bacterium solutions contain Aspergillus awamori, Aspergillus niger, Aspergillus tubingensis, penicillium citrinum, Aspergillus fumigatus, Eurotium rubrum, Aspergillus versicolor, Eurotium amstelodami, Paecilomyces variotii, Eurotium niveoglaucum, saccharomycetes and other beneficial microorganisms. On such basis, the inventor establishes the corresponding fermentation technique and equipment; and on the premise of not changing the upstream and downstream main production techniques of the original pile fermentation, the beneficial microorganism florae are added to control the growth of harmful infectious microbes, thereby enhancing the fermentation efficiency, shortening the pile fermentation period, and keeping the special thick flavor of the traditional Liupao tea. The pile fermentation automatic control system is utilized to dynamically monitor and control the changes on temperature, humidity and florae in the whole fermentation process, thereby implementing automation, continuity and high efficiency of the production technique, and ensuring the Liupao tea product to have safe and reliable quality and high and stable quality.
Owner:GUANGXI UNIV

Method capable of remarkably increasing monoester-type alkaloid of aconite roots

InactiveCN109620869AHigh active ingredientActive ingredients monoester type alkaloids increasePlant ingredientsPaecilomyces variotiiBiotechnology
The invention belongs to the technical field of medicine preparing, and particularly relates to a method capable of remarkably increasing monoester-type alkaloid of aconite roots. On the basis of traditional preparation of black prepared aconite roots, sieved pure breed microorganism bacterial suspension after steaming is fermented for 2 days, and strains in the microorganism bacterial suspensionare paecilomyces variotii. By adding the strains, the content of effective component monoester-type alkaloid of unprepared aconite roots can be increased by 11.11%, the aconite roots are prepared intothe black prepared aconite roots or white prepared aconite roots, compared with the content of monoester-type alkaloid in traditional black prepared aconite roots, the effect is best after paecilomyces variotii is added, and the content of monoester-type alkaloid of the aconite roots is increased by 23.18%. The preparation condition with addition of paecilomyces variotii is researched, and when preparing time is 48 h, the initial pH is 8 and temperature is 30 DEG C, the content of monoester-type alkaloid is increased by 47.7% compared with the traditional black prepared aconite roots.
Owner:SICHUAN ACAD OF FOOD & FERMENTATION INDS +1

Antibacterial mouldproof biaxially oriented polylactic film and preparation method thereof

The invention discloses an antibacterial mouldproof biaxially oriented polylactic film and a preparation method thereof. Based on 100 weight parts of biaxially oriented polylactic film special material, the polylactic film comprises the following blended components: 0.4 to 3 weight parts of antibacterial and mouldproof agent and 0 to 0.5 weight parts of dispersing agent; the film is prepared by a biaxial tension method and has the thickness of 10-100 mu m; and the antibacterial and mouldproof agent is a polyguanidine pyrithione antibacterial and moldproof agent, is prepared by mixing water-soluble polyguanidine inorganic acid salt or organic acid salt and sodium pyrithione in a molar ratio of 1:(0.1-5). The polylactic film has a good antibacterial and mouldproof effect, has the antibacterial effect of 99.9 percent on staphylococcus aureus and Escherichia coli, has the mouldproof grade of 0 for moulds such as aspergillus niger, aspergillus terreus, Paecilomyces variotii and the like, and has high water resistance and wide application prospect.
Owner:CHINA PETROLEUM & CHEM CORP +1

A Broad Spectrum Antifungal Bacillus and Its Application in Controlling Wheat Scab

The invention discloses broad-spectrum fungus-resistant bacillus and application thereof to preventing wheat scab and relates to bacillus. The preservation number of the bacillus (Bacillus sp.)DY26-010 is CCTCC NO:M2015407. Supernatant liquid of the bacillus is antagonistic to fusarium spp, gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia, mutualism alternaria, botrytis cinerea and colletotrichum dematium. Fermentative supernatant liquid of the bacillus DY26-010 has high antagonism on pathogenic bacteria and fusarium spp of wheat scab, the preventing effect can be 57.1% in a potted experiment of wheat scab, and the bacillus can be used for preparing the fermentative supernatant liquid applied to preventing wheat scab. A biological prevention method of wheat scab is provided, no chemical pesticide is used, the bacillus has the advantages of being non-toxic, not prone to forming a pesticide-resistant environment, biologically safe and the like, and application prospects are good.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION +1

Method for Detection of Paecilomyces Variotii

A method of detecting Paecilomyces variotii, containing identifying Paecilomyces variotii using a nucleic acid represented by the following nucleotide sequence (a) or (b):(a) a partial nucleotide sequence of a β-tubulin gene set forth in any one of SEQ ID NOS: 1 to 4 and 22 to 33, or a complementary sequence thereof; and(b) a nucleotide sequence which includes a nucleotide sequence set forth in any one of SEQ ID NOS: 1 to 4 and 22 to 33 which has a deletion, substitution, or addition of one to several nucleotides and can be used for detecting Paecilomyces variotii, or a complementary sequence thereof.
Owner:KAO CORP

A strain of Paecilomyces variotii with strong pathogenicity to citrus psyllids and its application

The invention discloses a Paecilomyces variotii strain with strong pathogenicity to citrus psyllids and an application thereof, belonging to the technical field of biological control. The strain is Paecilomyces variotii GZMS‑11, which was deposited in China Center for Type Culture Collection on October 29, 2015, with the preservation number CCTCC NO: M 2015651. Paecilomyces variotii GZMS-11 described in the present invention has strong pathogenicity to citrus psyllids, and can be developed as a biological pesticide for the biological control of citrus psyllids, compared with the current chemical agents for preventing and treating citrus psyllids , has the advantages of high efficiency, green, environmental protection, long-lasting effect and not easy to produce drug resistance, and has no pollution to the environment and no residue. The strain GZMS-11 has fast growth speed, large spore production, high spore germination rate, is easy to prepare, and has good market application prospects.
Owner:PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI

A kind of Paecilomyces variotii powder and its preparation method and application

The invention discloses paecilomyces variotii powder as well as a preparation method and an application thereof. The powder is prepared by fermenting paecilomyces variotii with a preservation number of CGMCC No.13772 on a corn flour culture medium. The paecilomyces variotii powder has the beneficial effects that (1) paecilomyces variotii can parasitize nematodes and simultaneously produce multiple secondary metabolites so as to inhibit the growth of the nematodes, so that the paecilomyces variotii powder can be used for effectively preventing and treating the nematodes; (2) the paecilomyces variotii powder is high in spore content and nematode killing efficiency and long in guarantee period; and (3) a preparation process of the paecilomyces variotii powder is simple and suitable for industrial production.
Owner:山东星之星农业科技有限公司

Anti-bacterium sanitary towel

The invention discloses an anti-bacterium sanitary towel which belongs to the field of health products. The anti-bacterium sanitary towel comprises an inner layer, an external layer and a packing layer, wherein the packing layer is located between the inner layer and the external layer, the inner layer is made of anti-bacterium silky thin non-woven fabric, high-concentration negative ion dust-free paper is arranged between the inner layer and the packing layer, and the packing layer is a liquid-absorbable soft filler. The anti-bacterium sanitary towel is made of the international unique anti-bacterium silky thin non-woven fabric which has extremely high functions of quick inhibition and sterilization on colon bacillus, golden staphylococcus, anaerobic bacteria and the like, wherein the killing rate is more than 99.99%, and the anti-bacterium silky thin non-woven fabric also has 0-1 grade bacteria resistant effect on aspergillus niger, aspergillus flavus, discolored aspergillus, penicillium curinum thom, trichoderma viride, chaetomium globasum, paecilomyces variotii and cladosporium herbarum. The inhibition and killing rate to the infectious viruses, such as syphilis, influenza virus, AH1N1 and the like is more than 90%, and the sterilization function effects within 10 minutes.
Owner:马志银

Method for preventing and controlling greenhouse soil continuous cropping obstacles by using endophytic fungus secondary metabolites

The invention discloses a method for preventing and controlling facility soil continuous cropping obstacles by using endophytic fungus secondary metabolites, and belongs to the technical field of facility horticulture. The secondary metabolite of the endophytic fungus Paecilomyces variotii strain SJ1 can effectively improve the crop system resistance, improve the resistance to continuous cropping soil stress such as soil acidification, secondary salinization, root-knot nematode and the like, promote root development, enhance the resistance of crops to the continuous cropping soil stress, relieve the harm of greenhouse soil continuous cropping obstacles, and improve the crop yield. And the method becomes an important technical means for green ecological prevention and control of greenhouse soil continuous cropping obstacles.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
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