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A kind of Paecilomyces variotii powder and its preparation method and application

A technology of Paecilomyces varostii and Penicillium powder, which is applied in the field of agricultural microorganisms, can solve the problems of low nematicide efficiency, short shelf life and low spore content, and achieves high nematicide efficiency, simple preparation process and high spore content. Effect

Active Publication Date: 2020-07-03
山东星之星农业科技有限公司
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AI Technical Summary

Problems solved by technology

[0002] In recent years, due to the continuous expansion of continuous cropping area and unscientific use of drugs, nematodes have developed and spread faster, their resistance has increased, and the rate of dead seedlings of crops has continued to increase. affected crop production
[0003] There are a variety of fungicides for nematode control on the market, but these fungicides have low nematodeicidal efficiency, short shelf life, and low spore content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] The present invention will be specifically introduced below in conjunction with specific embodiments.

[0019] Part 1: Obtaining Paecilomyces variotii

[0020] 1. Screen the isolated strains

[0021] The soil was collected from the greenhouse where the nematodes were infested, the soil was washed with water, and then the water samples were observed under a microscope, and the dead nematodes with hyphae were picked and transferred to the PDA plate for cultivation.

[0022] 2. Identification of strains

[0023] Using 28S for molecular detection, the detection results showed that the strain was Paecilomyces variotii.

[0024] 3. Strain preservation

[0025] The Paecilomyces wanzii we screened and isolated was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures (No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing) on ​​March 31, 2017, and the preservation number is CGMCCNo. 13772, with the Latin name Paeci...

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PUM

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Abstract

The invention discloses paecilomyces variotii powder as well as a preparation method and an application thereof. The powder is prepared by fermenting paecilomyces variotii with a preservation number of CGMCC No.13772 on a corn flour culture medium. The paecilomyces variotii powder has the beneficial effects that (1) paecilomyces variotii can parasitize nematodes and simultaneously produce multiple secondary metabolites so as to inhibit the growth of the nematodes, so that the paecilomyces variotii powder can be used for effectively preventing and treating the nematodes; (2) the paecilomyces variotii powder is high in spore content and nematode killing efficiency and long in guarantee period; and (3) a preparation process of the paecilomyces variotii powder is simple and suitable for industrial production.

Description

technical field [0001] The invention relates to a microbial powder and its preparation method and application, in particular to a Paecilomyces variotii powder and its preparation method and application, belonging to the technical field of agricultural microorganisms. Background technique [0002] In recent years, due to the continuous expansion of continuous cropping area and unscientific use of drugs, nematodes have developed and spread faster, their resistance has increased, and the rate of dead seedlings of crops has continued to increase. affected crop production. [0003] There are many fungal agents for nematode control on the market, but these fungal agents have low nematodecidal efficiency, short shelf life and low spore content. Contents of the invention [0004] In order to solve the deficiencies of the prior art, the object of the present invention is to provide a Paecilomyces variotii powder with high nematicidal efficiency, high spore content, long shelf life...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P5/00C12R1/79
CPCA01N63/30C12N1/14
Inventor 谢克虎
Owner 山东星之星农业科技有限公司
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