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One-strain high-ring polycyclic aromatic hydrocarbon degradation bacterium and uses thereof

A technology for polycyclic aromatic hydrocarbons and degrading bacteria, which is applied in the direction of fungi, microorganisms, biochemical equipment and methods, and can solve problems such as increasing research efforts

Inactive Publication Date: 2010-07-28
SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on other high-ring PAHs, and we should increase our research efforts in this area

Method used

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  • One-strain high-ring polycyclic aromatic hydrocarbon degradation bacterium and uses thereof
  • One-strain high-ring polycyclic aromatic hydrocarbon degradation bacterium and uses thereof
  • One-strain high-ring polycyclic aromatic hydrocarbon degradation bacterium and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: Paecilomyces variotii (Paecilomyces variotii) F 6 The screening separation and its PAH degradation performance.

[0013] Put 10g of fresh soil samples into a 250ml Erlenmeyer flask, add 90ml of sterilized sterile aqueous solution, and shake on a reciprocating shaker for 30min at 28°C (150r / min). After the solid particles have stood still for 30 minutes, take part of the suspension and dilute to obtain a series of suspensions 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 , respectively take 1ml and evenly spread on the agar plate containing high ring PAHs (three parallel samples), culture at 28°C. After 5-12 days of growth, single colonies with different characteristics (mycelium) and vigorous growth were selected and transferred to PAHs-free MYEA medium. The pure strains were obtained by streaking repeatedly on the solid medium plate. The purified strains were stored on a slant at 4°C. Fungal culture medium (MYEA): malt extract, 0.2%; yea...

Embodiment 2

[0016] Example 2: Effects of various growth factors on the degradation effect of high-ring PAH-degrading bacteria

[0017] 1. Select pH value, temperature, pollutant concentration, and C:N as the influencing factors (glucose is the source of C, (NH 4 ) 2 SO 4 N source), designed as L9(34) orthogonal experiment, the results are shown in Table 1.

[0018] 2. Insert the same number of dominant fungal spores into a series of Erlenmeyer flasks containing 50 mL of inorganic salt medium with different carbon-to-nitrogen ratios (the final number of spores in the medium is about 10 7 L -1 ), three repetitions were set; shake culture (150rpm, 28°C) for 5-7 days, and the degradation rate of ΣPAHs was measured, and the results are shown in Table 2.

[0019] The configuration method of above-mentioned inorganic salt medium is: inorganic salt basal medium (g·L -1 ): MgSO 4 ·7H 2 O, 0.2; CaCl 2 2H 2 O, 0.01; FeSO 4 ·7H 2 O, 0.005; KH 2 PO 4 , 0.4; Na 2 HPO 4 12H 2 O, 0.6; dis...

Embodiment 3

[0029] Example 3: Paecilomyces variotii F 6 Degradation effect on PAHs artificially polluted soil

[0030] 1. As shown in Table 4, the C of the artificial blending soil, the amount of N is relatively low (PAHs trace can be ignored), according to the optimal C: N ratio determined in Example 2, adding glucose and ammonium sulfate to adjust the content is C: 8g / kg, N: 0.8g / kg, adjust the moisture content to 60%-70%. Prepare nutrient solution: glucose: 19.2g / L, ammonium sulfate: 3.5g / L, deionized water 1L. (calculated on the basis of glucose 20g / kg).

[0031] Table 3 The content of each nutrient element in the soil

[0032] sample

Organic carbon (g / kg)

Total nitrogen (g / kg)

Available phosphorus (g / kg)

Available Potassium (mg / kg)

clean soil sample

4.16

0.43

0.007

0.9

[0033] 2. Take 12 parts of 50g soil, add 25ml / part of nutrient solution, that is, C: 8g / kg, N: 0.8g / kg (10:1), mix them evenly and put them into a sterilized pet...

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Abstract

The invention discloses an epipodium polycyclic aromatic hydrocarbon degrading bacterium and applications thereof. The epipodium polycyclic aromatic hydrocarbons degrading bacterium (paecilomyces variotii) F6 has the function of degrading a single pipodium polycyclic aromatic hydrocarbon and pipodium polycyclic aromatic hydrocarbon mixture. After the degrading bacterium is cultured for 30 days ina polycyclic aromatic hydrocarbon compound system, the degrading rate to the polycyclic aromatic hydrocarbon becomes 16.06 percent to 24.57 percent; however, the degrading rate in a single polycyclicaromatic hydrocarbons compound system becomes 10.42 percent to 33.25 percent, wherein, the degrading rates of benzo [k] fluoranthene and benzo [b] fluoranthene are the highest, which respectively account for 33.25 percent and 26.44 percent. The strain provided by the invention can provide new germplasm resources for developing the co-metabolism mechanism study of a plurality of epipodium polycyclic aromatic hydrocarbons as well as the construction of aromatic hydrocarbons combined pollution water-soil environment repairing technology.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a fungus for degrading high-ring polycyclic aromatic hydrocarbon components and its application in biological treatment of polluted soil and restoration of environmental pollution. Background technique [0002] PAHs are a class of hydrophobic organic pollutants, and low water solubility is the main reason for the persistence of these compounds. These compounds are of great concern due to their carcinogenic, teratogenic and mutagenic properties. In recent decades, the content of PAHs (polycyclic aromatic hydrocarbons) in the environment has been increasing, and the US EPA has listed 16 PAHs as priority control substances. Therefore, remediation of water and soil environments polluted by PAHs has become a research hotspot. [0003] Although PAHs can be degraded and transferred through chemical oxidation, photolysis and v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B09C1/10C12R1/79
Inventor 蔡信德李慧李伯威
Owner SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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