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Method for solid-state fermentation of cut tobacco stems and application thereof

A technology of solid-state fermentation and tobacco stems, which is applied in the fields of application, tobacco, and tobacco processing, can solve the problems of low utilization rate of tobacco stems, and achieve the effect of improving utilization rate and increasing aroma components.

Active Publication Date: 2020-09-18
CHINA TOBACCO SICHUAN IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of low utilization rate of tobacco stems, to provide a method for solid-state fermentation of shredded tobacco stems and its application, so as to degrade unfavorable substances in shredded tobacco stems that affect flavor, increase its aroma components, and improve the quality of tobacco stems. Utilization in cigarettes

Method used

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  • Method for solid-state fermentation of cut tobacco stems and application thereof
  • Method for solid-state fermentation of cut tobacco stems and application thereof
  • Method for solid-state fermentation of cut tobacco stems and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0048] (1) Cultivation of slant strains: inoculate Paecilomyces strains on the slant medium (i.e. PDA medium), and cultivate for about 7 days at 30°C until the mycelium covers the slant;

[0049] (2) Liquid seed culture: Inoculate the slant strain into a 250mL Erlenmeyer flask equipped with 80mL seed medium, and cultivate it for 7 days at a shaking table with a rotation speed of 150 rpm and 30°C;

[0050] (3) Paecilomyces fermented broth culture: After the liquid seeds were broken up with glass beads and a magnetic rotor, the liquid seeds were inoculated into the 250mL Erlenmeyer flask of 80mL Paecilomyces fermentation medium according to the volume percentage inoculum of 4%. , cultured at 30°C for 7 days at a shaker speed of 150 rpm;

[0051] (4) Solid-state fermentation culture: after the cultured Paecilomyces fermented liquid is broken up and mixed, take the cultivated Paecilomyces fermented liquid and inoculate it into tobacco stem shreds at an inoculum size of 10% (V / m). ...

Embodiment 2

[0057] Paecilomyces fermented liquid inoculum size is 20% (V / m), and other conditions are the same as embodiment 1.

[0058] Test results: after 2 days, 4 days, and 6 days of fermentation, the changes in components of cut tobacco stems are shown in Table 2. After 6 days of fermentation, the degradation rate of cellulose was 13.75%, the degradation rate of hemicellulose was 14.77%, and the degradation rate of lignin was 11.37%. , the degradation rate of pectin was 15.12%, the increasing rate of reducing sugar content was 34.49%, and the increasing rate of total sugar was 30.99%.

[0059] Table 2:

[0060]

Embodiment 3

[0062] Paecilomyces fermented liquid inoculum size is 30% (V / m), other conditions are the same as embodiment 1.

[0063] Test results: after 2 days, 4 days, and 6 days of fermentation, the changes in components of cut tobacco stems are shown in Table 3. After 6 days of fermentation, the degradation rate of cellulose was 6.62%, the degradation rate of hemicellulose was 18.47%, and the degradation rate of lignin was 18.63%. , the degradation rate of pectin was 14.87%, the increasing rate of reducing sugar content was 13.97%, and the increasing rate of total sugar content was 10.35%.

[0064] table 3:

[0065]

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Abstract

The invention discloses a method for solid-state fermentation of cut tobacco stems, and belongs to the technical field of microbial fermentation. According to the method, solid-state fermentation is carried out on cut tobacco stems by using paecilomyces varioti fermentation liquor, and the method mainly comprises the following steps of: (1) slant strain culture; (2) liquid seed culture; (3) culture of paecilomyces varioti fermentation broth; and (4) solid-state fermentation culture, which is implemented by inoculating a cultured paecilomyces varioti fermentation liquid into a solid-state fermentation culture medium, and culturing the paecilomyces varioti fermentation liquid at the temperature of 22-32 DEG C for 6-10 days. The cut tobacco stems are subjected to fermentation and biotransformation of paecilomyces varioti strains, so that cell wall substances in the cut tobacco stems are decomposed and utilized by the paecilomyces species, efficient bioactive substances such as paecilomyces polysaccharide and paecilomyces acid are generated, adverse factors influencing the internal quality and flavor of the cut tobacco stems are reduced, the sensory quality during smoking is improved,and the utilization rate of the tobacco stems in cigarettes is increased.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and relates to a method for solid-state fermentation of shredded tobacco stems and an application thereof. Background technique [0002] As a big tobacco country, my country ranks first in the world in terms of planting area and output of tobacco, and a large amount of tobacco stems are also produced therefrom. At present, the methods for processing tobacco stems mainly include physical blasting, chemical treatment, and biological enzyme methods, which have disadvantages such as poor degradation effect, chemical reagent pollution, and high cost. At present, it is reported that Bacillus pumilus V35 is used to make a bacterial agent for the treatment of cut stems, but it only degrades cellulose and pectin, and its cell wall component lignin is not degraded, so the degradation effect of its cell wall substances is not obvious. At present, the utilization rate of tobacco stems is low,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20
CPCA24B15/20
Inventor 李东亮刘文莉马亚萍张娟丁重阳朱鹏程陈坚堵国成
Owner CHINA TOBACCO SICHUAN IND CO LTD
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