Rapid detection method for content of adenosine in paecilomyces hepialid mycelium powder

Abstract

The invention relates to a rapid detection method for content of adenosine in paecilomyces hepialid mycelium powder. The rapid detection method is characterized by comprising the following steps: 1) preparing adenosine standard solution, namely weighing an adenosine standard substance, dissolving and carrying out constant volume with adenosine extracting solution, and preparing the adenosine standard solution for later use; 2) preparing sample solution, namely weighing a paecilomyces hepialid mycelium powder sample, adding the adenosine extracting solution, dissolving, uniformly mixing, addingquartz sand and grinding to be pasty, cooling, then transferring into a colorimetric tube, carrying out constant volume, carrying out ultrasonic oscillation by virtue of an ultrasonic oscillator, mixing, freezing in an environment at the temperature of -20 DEG C, then melting and transferring into a centrifugal machine to be subjected to centrifugal treatment, taking supernatant, filtering by virtue of a 0.45Mum filtration membrane, and preparing the sample solution for later use; and 3) carrying out detection and analysis by virtue of high performance liquid chromatography. The rapid detection method provided by the invention is scientific and reasonable in design, good in adenosine chromatographic peak separation effect, simple in mobile phase, high in adenosine recovery rate and good in repeatability and has the advantages of easy operation, shorter time, high detection speed and high detection efficiency.

Description

technical field [0001] The invention belongs to the field of detection of active components of health food, and relates to the detection of active components of Paecilomyces basilicum mycelia powder, in particular to a rapid detection method for adenosine content in the mycelia powder of Paecilomyces baculata. Background technique [0002] Cordyceps sinensis is a medicinal fungus of the family Ergotaceae, which is rich in physiologically active substances, has unique medicinal value and extensive pharmacological effects, and is a rare and precious Chinese herbal medicine specially produced in China. In recent years, people have isolated a variety of biologically active substances from the fungal mycelium of Cordyceps sinensis, mainly including adenosine, Cordyceps polysaccharides, D-mannitol, sterols and other components. These main components have anti-inflammatory, anti-oxidation, anti-tumor, enhance Immunity and other physiological functions. [0003] At present, the art...

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Problems solved by technology

Both of the above two methods use a single method of direct ultrasound to extract adenosine, which has the problem of low extraction efficiency of adenosine

Moreover, the former is less polar because the extract is 60% ethanol, while the polarity of adenosine in the mycelia powder of Paecilomyces hematalis is relatively large, and there are problems such as insufficient extraction of active ingredients and inaccurate determination of adenosine content. The latter uses a phosphate buffer solution containing 2% tetrahydrofuran (0.066 mol / L potassium dihydrogen phosphate: 0.066 mol / L disodium hydrogen phosphate = 2:3) as the mobile phase when performing liquid chromatography detection, which is cumbersome to operate. , the mobile phase is relatively complex, and the equipment loss is large, so it cannot meet the requirements of rapid product inspection

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