87 results about "Aspergillus versicolor" patented technology

The current invention relates to the field of detection and identification of clinically important fungi. More particularely, the present invention relates to species specific probes originating from the Internal Transcribed Spacer (ITS) region of rDNA for the detection of fungal species such as Candida albicans, Candida parapsilosis, Candida tropicalis, Candida kefyr, Candida krusei, Candida glabrata, Candida dubliniensis, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Aspergillus fumigatus, Cyptococcus neoformans and Pneumocystis carinii in clinical samples, and methods using said probes.

The present invention relates to a microorganism, a microbial phosphate fertilizer and a method for manufacturing said microbial phosphate fertilizer, wherein said microorganism is Streptomyces cellulosae with Accession number CGMCC No. 2167 or Aspergillus versicolor with Accession number CGMCC No. 2171. The microbial phosphate fertilizer of the present invention comprises a fermentation product of the microbial composition consisting of the following four microorganisms: Bacillus subtilis strain WH2, Bacillus licheniformis strain WH4, Streptomyces cellulosae strain WH9 and Aspergillus versicolor strain WH13. A method for manufacturing said microbial phosphate fertilizer is also provided, wherein the manufacture of said microbial phosphate fertilizer may involve the use of ground phosphate rock having 8%-12% P2O5.

The invention discloses a furanone compound, a preparation method and application thereof. The furanone compound is characterized by having a structural formula shown as formula I. The preparation method includes the steps of: conducting fermentation culture on aspergillus versicolor with a preservation number of CCTCC No. M2014089 to obtain a fermentation product containing a new furanone compound, then soaking the fermentation product with methanol, and performing extraction with ethyl acetate to obtain a crude extract, subjecting the crude extract to decompression silica gel column chromatography and conducting reversed-phase semi-preparative high performance liquid chromatography separation and purification, thus obtaining the product. The furanone compound has antifungal effect, and can be used as an antifungal new drug component or lead compound.

The invention provides a bacterial strain capable of producing chitin deacetylase and an application of bacterial strain in production of chitin deacetylase through fermentation. The bacterial strain is Aspergillus versicolor X, and is preserved at a China Center for Type Culture Collection, the preservation number is CCTCC NO.M2014459, and preservation date is 8th, Oct, 2014. The invention also provides a enzyme production method through fermentation, the enzyme activity of the prepared chitin deacetylase can reach 1.41U / mL. The application has the advantages that scope of enzyme microbe capable of producing chitin deacetylase is developed, production cost is reduced, and chitin deacetylase has wide application prospect.

The invention relates to the fields of algistats, pesticides and bacteriostatic agents, in particular to a chloro alkaloid compound which is a source of seaweed endophytic fungi and the preparation and application of the chloro alkaloid compound. The specific structural formula is shown in I, and the preparation method comprises the following steps: inoculating Aspergillus versicolor DL-29 into a fungi liquid medium for fermental cultivation, and purifying the fermentation product to obtain the chloro alkaloid compound shown in the formula I. An algistatic activity experiment shows that the median inhibitory concentration of the chloro alkaloid compound is 6.3 microgram / ml, and the compound has insecticidal and bacteriostatic activities.

The invention provides an Aspergillus versicolor SD-3 and its application in the preparation of chitin deacetylase. The Aspergillus versicolor SD-3 has simple nutrition requirements, has strong competitor pollution resistance, and is easy to culture; chitin deacetylase generated by the Aspergillus versicolor SD-3 is lyoenzyme, a crude chitin deacetylase liquid can be obtained after separating and removing the Aspergillus versicolor SD-3, and the crude chitin deacetylase liquid can be directly used, and is simple to prepare; the deacetylase produced by the Aspergillus versicolor SD-3 has high activity, and the vitality of the non-separated and non-purified crude deacetylase liquid reaches 142.3U / mL under optimal conditions; and a production technology of the chitin deacetylase does not use toxic harmful raw materials, so environmental protection and no pollution are realized.

The invention provides Xanthone compounds and a preparation method thereof, and application of the Xanthone compounds in the field of agriculture, belonging to the field of fungus extracts. A fermentation extract is obtained from Aspergillus versicolor D5 derived from ocean, and 15 compounds are obtained through separation and extraction, wherein 6 compounds are identified as new compounds, and 9compounds are identified as known compounds. The resuts of herbicidal activity tests and antifungal activity tests show that the Xanthone compounds have good antifungal and herbicidal activity, are environment-friendly, safe and efficient, and have better or equivalent control effects compared with chemical pesticides.

The invention discloses an Aspergillus-versicolor-derived alpha-galactosidase Z-GALC, and a coding gene and application thereof. The protein Z-GALC has alpha-galactosidase activity, is derived from Aspergillus versicolor, and is (a) or (b) as follows: (a) protein composed of amino acid sequence disclosed as Sequence 1 in the sequence table; or (b) protein derived from Sequence 1 in the sequence table with alpha-galactosidase activity, which is subjected to substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence disclosed as Sequence 1 in the sequence table. The specific activity of the protein Z-GALC disclosed by the invention as the alpha-galactosidase is 4.07U / mg; the optimum pH value is 7.0; the optimum temperature is 42 DEG C; and the protein Z-GALC has high enzyme activity at the temperature of 37-50 DEG C under the pH value of 6.0-8.0. The invention provides a new way for preparing alpha-galactosidase, and has important application value.

The invention relates to an Aspergillus versicolor for fermenting a Jatropha cake to manufacture biological bacterial manure, belonging to the field of manure. The Aspergillus versicolor provided by the invention is named as Aspergillus versicolor A003. The stain is preserved in a preservation unit designated by State Intellectual Property Office, the preservation date is 18th, July 2011, the name of the preservation unit is China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2011240. When the Aspergillus versicolor is inoculated on a culture medium mainly prepared from the Jatropha cake, the Jatropha cake can be quickly fermented, toxic components in the Jatropha cake can be eliminated, the detoxified Jatropha cake and live thalli growing on the Jatropha cake can be used as biological bacterial manure and can promote the growth of various crops.

The invention belongs to a classification and identification method upon 12 common fungi, and specifically relates to a method for carrying out classification and identification upon 12 common fungi such as F.graminearum, A.terricola, P.citreonigrum, A.flavus, A.parasiticus, P.aurantiogriseum, T.pseudokoningii, A.humicola, P.viridicatum, A.funigatus, A.uersicolor, and F.solani by using a Fourier transform infrared spectroscopic (FTIR) technology combined with chemometric operation (hierarchical cluster analysis). The method comprises the steps of the establishment of a FTIR spectra information database of the 12 fungi, fungi culturing, ZnSe window manufacturing, spectral acquisition and preprocessing, and result determination by hierarchical cluster analysis. The method has the advantages of high resolution, high speed, simple operation, and low cost.

The invention discloses an aspergillus sp. Strain GW13 CGMCC No.19931 and application of the aspergillus sp. Strain GW13 in preparation of sterigmatocystin. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 16th, 2020 (CGMCC for short), the address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing), and the registration number of the preservation center is CGMCC No.19931. The industrial technical problem of preparing the high-purity sterigmatocystin standard substance is effectively solved.

The invention discloses a tetrahydrofuran compound and a preparation method and application thereof. The invention is characterized in that the structural formula of the tetrahydrofuran compound is as shown in the formula I. The preparation method comprises the following steps: acquiring a fermented substance containing a novel tetrahydrofuran compound by fermental cultivation of aspergillus versicolor with the preservation number of CCTCC No. M2014089; immersing the fermented substance with methanol and extracting with ethyl acetate so as to obtain a crude extract; and separating and purifying the crude extract by reduced pressure silica-gel column chromatography and semi-preparative reversed phase high-performance liquid chromatography. The tetrahydrofuran compound has an antifungal effect and can be used as a novel medicine component or lead compound with an antifungal effect.

The invention relates to an anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human colon cancer resisting drugs. The compound plays a role in inhibiting proliferation of colon cancer cells. The compound has a structural formula shown in the description. The compound is obtained through carrying out fermentation culturing on aspergillus versicolorIBPT-7 so as to obtain a fermented product and then separating the compound from the fermented product and purifying the compound. Confirmed by experiments, the compound has relatively good anti-tumor activity to human colon cancer cells, i.e., HCT-116. The compound can be applied to research on human colon cancers as a human lung cancer cell proliferation inhibiting drug or anti-tumor drug.

The invention provides a microbial agent for fermenting Chinese herb residues. The microbial agent for fermenting Chinese herb residues is composed of two kinds of bacterial strains including streptomyces BY1 and aspergillus versicolor BM13. The microbial agent can improve the temperature of compost, accelerate moisture evaporation, rapidly start degradation of cellulose and lignin, increase the degradation rate of cellulose and promote compost maturity under the conditions of not adjusting the pH value and C / N of Chinese herb residues and having high moisture content (greater than 70%).

The invention belongs to the field of medical biology, and relates to five antifungal compounds (as shown in formulas I-V) which are obtained from fermentation products of marine-derived aspergillus versicolor by adopting a co-culture (also known as mixed culture) technology, and a preparation method of the antifungal compounds. The invention also relates to an application of the compound in resisting fungal infection.

The invention discloses a biphenyl compound, a preparation method and uses thereof. The preparation method comprises: carrying out fermentation culture on Aspergillus versicolor with a preservation number of 3A00080 to obtain a fermentation broth containing a biphenyl compound and hypha, extracting the fermentation broth by using ethyl acetate to obtain a crude extract, carrying out pressure reducing silica gel column chromatography on the crude extract, carrying out reverse phase medium pressure column chromatography, and carrying out separating purification through reverse phase semi-preparative high performance liquid chromatography to obtain the biphenyl compound. According to the present invention, the biphenyl compound can inhibit Saccharomyces cerevisiae, and can strongly inhibit Bacillus subtilis and Staphylococcus aureus.

The invention relates to an anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human esophageal cancer resisting drugs. The compound plays a role in inhibiting human esophageal cancer cell proliferation. The compound has a structural formula shown in the description. The compound is obtained through carrying out fermentation culturing on aspergillus versicolor IBPT-7 so as to obtain a fermented product and then separating the compound from the fermented product and purifying the compound. Confirmed by experiments, the compound has relatively good anti-tumor activity to human esophageal cancer cells, i.e., EC9706. The compound can be applied to research on human esophageal cancers as a human esophageal cancer cell proliferation inhibiting drug or anti-tumor drug.

The invention discloses aspergillus versicolor and the application thereof in preparing podophyllotoxin mono-glucoside and podophyllotoxin. The preservation number of the aspergillus versicolor W1 is CGMCC NO.8571. The invention further provides application of the aspergillus versicolor W1 in preparing the podophyllotoxin mono-glucoside and / or the podophyllotoxin. The invention also provides a method for preparing the podophyllotoxin mono-glucoside and / or the podophyllotoxin. The method comprises the following steps: fermenting and culturing the aspergillus versicolor W1 so as to obtain the podophyllotoxin mono-glucoside and / or the podophyllotoxin. The podophyllotoxin mono-glucoside and the podophyllotoxin can be obtained after fermenting and culturing the aspergillus versicolor disclosed by the invention, the operation is simple, the cost is low, the production cycle is short, and the application potential for industrialized large-scale production is achieved. The invention provides a new way for the resource development of podophyllotoxin substances, and an important significance is realized in protection for the ecological diversity of plants.

The invention discloses a preparation method of a building coating. The building coating has good polishability, good adhesiveness and a good waterproof property, and is composed of boric acid, a styrene acrylic emulsion, benzyl bromoacetate, magnesium oxide, polyvinyl alcohol, polyurethane resin, titanium dioxide, a film forming assistant, sodium hexametaphosphate, a dispersant, a thickening agent, heavy-calcium-and-silicon pulverized lime, talcum powder and an antifoaming agent. The building coating adopting an efficient antiseptic can effectively inhibit Aspergillus niger, Aspergillus flavus, Aspergillus versicolor and the like. The coating has the advantages of good mildew resistance, harmlessness to human bodies, low cost, extremely strong adhesion, extremely strong water and ultraviolet resistances, and no peeling, shedding or color change after long-term use in wet environment.

The invention provides an Aspergillus versicolor strain for manufacturing detoxicated feedstuff through the fermentation of Jatropha curcas cakes, relating to the field of feedstuff. In the invention, the Aspergillus strain is named Aspergillus versicolor A003; the strain was preserved by China Center for Type Culture Collection (a preservation unit specified by Patent Office of State Intellectual Property Office) on July 11th, 2011; and the preservation number is CCTCC NO:M2011249. By inoculating the strain onto a culture medium prepared from Jatropha curcas cakes used as main components, the Jatropha curcas cakes can be fermented quickly, and toxic components in the Jatropha curcas cakes can be removed; and the detoxicated Jatropha curcas cakes can be used as protein feedstuff for feeding animals.

The invention belongs to the technical field of natural product chemistry, and particularly relates to an indole alkaloid compound as well as a preparation method and application thereof. The molecular formula of the compound is C15H15NO2, and the compound has the following structure shown in the specification. The invention also discloses a preparation method and application of the compound. The compound disclosed by the invention is a novel compound separated from a fermentation product of endogenous aspergillus versicolor of tobacco, and is identified as the indole alkaloid compound through spectrum data such as nuclear magnetic resonance, mass spectrum, infrared and the like. The indole alkaloid compound has good tobacco mosaic virus resisting activity, and it is found through tobacco mosaic virus resisting activity tests that the relative inhibition rate of the indole alkaloid compound to tobacco mosaic viruses reaches 41.2%, and the activity of the indole alkaloid compound is higher than the relative inhibition rate (32.5%) of a reference substance ningnanmycin. The compound disclosed by the invention is simple in structure and good in activity, can be used as a leading compound of the tobacco mosaic virus resisting medicine, and has a good application prospect in preparation of the tobacco mosaic virus resisting medicine.

The invention provides endophytic strains with high camptothecin yield. The endophytic strains with high camptothecin yield are Alternaria temuissima ZCMUKL-S1 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M2021189, or Alternaria citricancri ZCMUKL-S2 with the preservation number of CCTCC NO: M2021190, or Aspergillus versicolor ZCMUKL-S3 with the preservation number of CCTCC NO: M2021191. The endophytic fungi have high camptothecin yield, the camptothecin yield of unit dry mycelium powder of the Aspergillus versicolor ZCMUKL-S3 is up to 116 [mu]g / g, which is the highest yield of camptothecin produced by the endophytic fungi reported at present, and the endophytic fungi have potential application value for industrial production of camptothecin. In addition, the endophytic fungi with high camptothecin yield provided by the invention have better genetic stability.

The invention relates to the technical field of microorganisms, in particular to a complex microbial inoculant for efficient degradation of straw and a preparation method and application of the complex microbial inoculant. The complex microbial inoculant comprises a flora A, a flora B and a flora C; the flora A comprises aspergillus fumigatus, aspergillus versicolor, trichoderma harzianum, phanerochaete chrysosporium and auricularia polytricha; the flora B comprises bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens; and the flora C comprises depubyomyces hansenii, pichia guilliermondii, bacillus subtilis, bacillus cereus and aneurinibacillus. The flora A is used for preparing a microbial agent I, the flora A and the flora B are used for preparing a microbial agent II, the flora A and the flora C are used for preparing a microbial agent III, and the flora A, the flora B and the flora C are used for preparing a microbial agent IV. According to the complex microbial inoculant, the flora A which is close to the environment and has a lignocellulose degradation function is preferably selected from the natural environment by a traditional microbiological method, and the flora B and the flora C are combined to achieve a synergistic effect, so that the straw degradation efficiency is effectively improved, and the complex microbial inoculant has important guiding significance on straw resource utilization.

The invention discloses aspergillus versicolor ZJB16085 and an application of the aspergillus versicolor to the synthesis of R-2-(4-hydroxyphenoxy) propionic acid. The preservation number of the aspergillus versicolor is CCTCC NO: M 2018408. The present invention separates out the aspergillus versicolor CCTCC NO: M 2018408 from humic soil, and the aspergillus versicolor can efficiently convert R-2-phenoxypropionic acid and synthesize R-2-(4-hydroxyphenoxy) propionic acid, and can synthesize 4.5 g / L R-2-(4-hydroxyphenoxy) propionic acid in the presence of 10 g / L substrate, wherein the conversion rate is 45%.

The invention relates to a non-toxic aspergillus flavus strain and application thereof. A non-toxic aspergillus flavus XZCY1805 disclosed by the invention does not produce various toxins such as aflatoxin (AFT), cyclopiamic acid (CPA), aspergillus flavus tremorine (Aflatrem), sterigmatocystin (ST) and the like, and is a biocontrol strain with high safety and strong competitive advantage. The non-toxic aspergillus flavus XZCY1805 disclosed by the invention has a strong toxic inhibition effect on a standard toxic aspergillus flavus strain 3.4408 and toxic aspergillus flavus separated from different peanut production places, and can remarkably reduce the contents of the aflatoxin, the cyclopiamic acid, the aspergillus flavus tremorin and the sterigmatocystin in agricultural products such as peanuts and the like.

The invention discloses 3,4,6-trisubstituted-alpha-pyrone derivative and preparation and application thereof. The 3,4,6-trisubstituted-alpha-pyrone derivative is characterized in that a structural formula of the 3,4,6-trisubstituted-alpha-pyrone derivative is shown as in formula I, and a preparation method comprises the steps of subjecting Aspergillus versicolor with a preservation number of CCTCCNo. M2014089 to fermentation culture to obtain a fermented product containing novel 3,4,6-trisubstituted-alpha-pyrone derivative, soaking the fermented product in methanol, extracting with ethyl acetate to obtain crude extract, subjecting the crude extract to reduced pressure silica-gel column chromatography, performing reversed-phase semi-preparative high-performance liquid chromatography, separating, and purifying to obtain the 3,4,6-trisubstituted-alpha-pyrone derivative. The 3,4,6-trisubstituted-alpha-pyrone derivative can resist Botrytis cinerea and has the advantage that the derivativemay act as a novel pharmaceutical ingredient or lead compound for Botrytis cinerea inhibitors.

The invention provides non-toxin-producing aspergillus flavus SX0104 and application thereof, and particularly relates to the technical field of microorganisms. According to the provided non-toxin-producing aspergillus flavus SX0104, the preservation number of the aspergillus flavus SX0104 is CCTCC NO:M2020520. The aspergillus flavus SX0104 does not produce aflatoxin, aspergillus flavus tremorin or sterigmatocystin, is a biocontrol strain with high safety and strong reproductive capacity, and has the capabilities of efficiently and competitively inhibiting the growth, reproduction and toxin production of toxin-producing aspergillus flavus; and the content of the aflatoxin, the aspergillus flavus tremorin and the sterigmatocystin in agricultural products such as peanuts can be obviously reduced.