34 results about "Protein Z" patented technology

The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, comprising at least the mutation wherein the glutamine residue at position 9 has been mutated to a tryptophan, leucine, glutamic acid, valine or lysine. The invention also discloses multimers of the polypeptide, as well as separation matrices comprising the multimers or polypeptides.

The invention discloses a polypeptide capable of binding immunoglobulins or immunoglobulin-containing proteins, which polypeptide comprises six or more domains of protein Z or the C domain of protein A or a functional variant thereof. It also discloses separation matrices comprising the polypeptide and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin-containing proteins.

The invention relates to the technical field of chlorogenic acid purification, in particular to a purification process of chlorogenic acid in corn vinasse. The purification process comprises the stepsthat under the action of ultrasonic dispersion, cellulose, polygalacturonase and a protein Zeah can all be evenly dispersed in a mixed component, the contact probability between the cellulose, the polygalacturonase as well as a the protein Zeah and corn cell walls is increased to the great extent, and thus the enzymolysis efficiency on the corn cell walls is improved; the permeability of corn cells is increased under the action of calcium ions, and through cooperative use of an ultrasonic cell crusher, the crushing efficiency of the corn cells is improved greatly; under cooperative use actionof complex enzymes, ultrasonic dispersion, a calcium ion solution and the ultrasonic cell crusher, the chlorogenic acid flows out to the maximum limit; and then the chlorogenic acid is preliminarilypurified, namely lixiviated, through an ethanol solution, a crude chlorogenic acid product is prepared, then the crude chlorogenic acid product is eluted through ethyl acetate, finally, the chlorogenic acid is recrystallized through an organic solvent, and thus the purity of the prepared chlorogenic acid is improved, and yield of the prepared chlorogenic acid is increasedpurity and yield of the prepared chlorogenic acid are increased.

The invention discloses a centromere associated protein and encoded gene as well as application thereof, and aims to provide the centromere associated protein and the encoded gene as well as the application in preparing drug for inhibiting tumor proliferation. The protein is one of the following amino acid sequences: 1) the amino acid sequence indicated in SEQ ID NO.1; or 2) the amino acid residue sequence of the SEQ ID NO.1 is replaced by one or multiple amino acid residues, lost and added so as to be the amino acid sequence that is derived from the SEQ ID NO.1 and has the regulating and controlling functions to the process of the cell mitosis as combined with the CENP-E protein. The protein participates in the spindle checkpoint signal transduction route of the cell mitosis through the mutual function with the CENP-E, and the active ingredient of the protein can be taken as the drug target for developing the drug for inhibiting tumor proliferation. The protein and the encoded gene thereof play a significant role in the field of medicine and pharmacy, and have a wide application range.