Complex microbial inoculant for efficient degradation of straw and preparation method and application of complex microbial inoculant

Abstract

The invention relates to the technical field of microorganisms, in particular to a complex microbial inoculant for efficient degradation of straw and a preparation method and application of the complex microbial inoculant. The complex microbial inoculant comprises a flora A, a flora B and a flora C; the flora A comprises aspergillus fumigatus, aspergillus versicolor, trichoderma harzianum, phanerochaete chrysosporium and auricularia polytricha; the flora B comprises bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens; and the flora C comprises depubyomyces hansenii, pichia guilliermondii, bacillus subtilis, bacillus cereus and aneurinibacillus. The flora A is used for preparing a microbial agent I, the flora A and the flora B are used for preparing a microbial agent II, the flora A and the flora C are used for preparing a microbial agent III, and the flora A, the flora B and the flora C are used for preparing a microbial agent IV. According to the complex microbial inoculant, the flora A which is close to the environment and has a lignocellulose degradation function is preferably selected from the natural environment by a traditional microbiological method, and the flora B and the flora C are combined to achieve a synergistic effect, so that the straw degradation efficiency is effectively improved, and the complex microbial inoculant has important guiding significance on straw resource utilization.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a compound bacterial agent for efficiently degrading straw and its preparation method and application. Background technique [0002] my country is a big rice producing country in the world, and straw is an important biomass resource remaining after crop harvest. In view of the unique structure and complex composition of straw, when it is used as a resource, physical, chemical, biological and other means are often required to pretreat it to change its dense structure and separate components such as cellulose, lignin, and hemicellulose. To increase the degradation rate of straw. The physical method changes the internal structure of straw by means of crushing and extrusion, steam explosion, etc. to improve its utilization rate, but there are problems such as high equipment construction and operation costs, and large energy utilization. The chemical method reduces the conten...

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Problems solved by technology

However, this bacterial agent only uses some single functional bacteria, and there are problems such as low enzyme activity of the strain, harsh application conditions, and unstable degradation effect.

[0004] Another example is a lignocellulose-degrading composite bacterial agent disclosed in Chinese Patent Publication No. CN111849803A, which is a mixture of livestock and poultry manure and straw. The composite bacterial agent has a concentration of 10 9 The preservation number of each / mL is the fermentation broth of Rhizomucor miehei NJAU-F4-5 of CGMCC No.18135 and the concentration is 10 9 The preservation number of CFU / mL is CGMCC No.18020 Brevibacillus reuteri NJAU-N20 fermentation broth composed of equal volumes; the composite bacterial agent improves the production efficiency of organic fertilizer, and the degradation rate of the composite bacterial agent to straw only reaches 20.55% %, the degradation time and degradation effect are poor

[0005] In addition, as disclosed in Chinese Patent Publication No. CN110699289A, a preparation method and application of a high-efficiency straw-degrading composite bacterial agent includes: S1 collecting vegetable straws and cutting them into pieces to obtain straw waste A; S2 making straw waste A, cultivation soil , nitrogen source and water are evenly mixed to obtain mixture B; S3 takes mixture B, and inoculates EM bacteria liquid, yeast liquid, Bacillus subtilis bacteria liquid and Trichoderma harzian bacteria liquid into mixture B, and then stirs Ferment for 3 to 5 days after mixing; the straw-degrading composite bacterial agent can achieve the effect of improving the antagonistic effect of the dominant growth flora and increasing the diversity of the flora and microorganisms, but in the actual use of the bacterial agent, physical and chemical methods are still required. Pretreatment of materials increases the cost and difficulty of operation, and it is difficult to meet the requirements of industrial application of compound bacterial agents

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