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Anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human esophageal cancer resisting drugs

A technology of Aspergillus versicolor and compounds, applied in the field of biomedicine, can solve problems such as drugs that have not yet been seen, and achieve the effect of inhibiting the proliferation activity of human esophageal cancer and significantly improving the proliferation activity of human esophageal cancer

Active Publication Date: 2018-02-27
FUJIAN HEALTH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that the anthraquinone compounds shown have anti-human esophageal cancer activity, and there is no report on the anti-proliferation activity of this compound on human esophageal cancer cells, so there are no related drugs on the market.

Method used

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  • Anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human esophageal cancer resisting drugs
  • Anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human esophageal cancer resisting drugs
  • Anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human esophageal cancer resisting drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Fermentative production and separation and purification of the compound of embodiment 1

[0020] 1 Fermentation production

[0021] Fermentation culture of production bacteria: According to the conventional method of cultivating microorganisms, take Aspergillus versicolor ( Aspergillus versicolor ) IBPT-7 (preserved in China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number is: CCTCC NO: M 2013715) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 2 to 3 days.

[0022] Take an appropriate amount of Aspergillus versicolor cultivated on a slope for 2 to 3 days, inoculate it into the SWS culture medium, and after 30 days of static culture at 28°C, obtain 20 L of mycelia and fermentation liquid; the composition of the culture liquid: per liter of water contains 10.0 g of starch, peptone 1.0 g, NaCl 20.0 g.

[0023] 2 Obtaining the extract

[0024] The Aspergillus versic...

Embodiment 2

[0029] Example 2 Test of anti-tumor activity in vitro

[0030] 1 Experimental samples and experimental methods

[0031] Preparation of test sample solution The test sample is the pure product of the compound isolated and refined in the above-mentioned Example 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0032] The subculture of cell lines and cells adopts tumor cell lines, and the tumor cells are treated with DMEM medium containing 10% FBS at 37°C with 5% CO 2 Subculture in an incubator.

[0033] Cell Proliferation Inhibitory Activity Test Method

[0034] Tetrazolium salt (MTT) method Take tumor cells in the logarithmic growth phase and adjust the cell density to 1×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 μL per well, and placed in 5% CO at 37°C. 2 Incubate for 4 h in an incubator. Add 2 μL of sample solution or blank solution to each ...

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Abstract

The invention relates to an anthraquinone compound derived from aspergillus versicolor and application thereof in preparation of human esophageal cancer resisting drugs. The compound plays a role in inhibiting human esophageal cancer cell proliferation. The compound has a structural formula shown in the description. The compound is obtained through carrying out fermentation culturing on aspergillus versicolor IBPT-7 so as to obtain a fermented product and then separating the compound from the fermented product and purifying the compound. Confirmed by experiments, the compound has relatively good anti-tumor activity to human esophageal cancer cells, i.e., EC9706. The compound can be applied to research on human esophageal cancers as a human esophageal cancer cell proliferation inhibiting drug or anti-tumor drug.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to an anthraquinone compound derived from Aspergillus versicolor and its application in the preparation of anti-human esophageal cancer drugs. Background technique [0002] Anthraquinones are widely used in natural medicines and have important pharmacological effects. For example, Chinese medicine Polygonatum, Comfrey, Salvia, Rhubarb, Tripterygium and other plants contain a large amount of anthraquinones, which have anti-tumor, antibacterial, and antioxidant properties. It has multiple functions, especially in the prevention and treatment of major diseases such as cancer, cardiovascular disease, senile dementia, and AIDS. Among them, in the field of anti-tumor, its mechanism of action is mainly manifested in inducing tumor cell apoptosis, reversing the multidrug resistance of tumor cells, enzyme inhibitors, affecting the proliferation and cell cycle of tumor cells, inhibiting ...

Claims

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Application Information

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IPC IPC(8): C07D313/20C12P17/08A61P35/00C12R1/66
CPCC07D313/20C12P17/08
Inventor 倪敏林燕喃张丽华陈筱瑜唐小峦
Owner FUJIAN HEALTH COLLEGE
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