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Penicillium citrinum-derived penicitrinine A as well as application thereof to preparation of drugs for resisting nasopharyngeal carcinoma

A technology derived from Penicillium citrinum, applied in the field of medicine

Inactive Publication Date: 2015-11-18
福建省肿瘤医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the alkaloid compound shown has anti-nasopharyngeal carcinoma activity, and there is no report on the anti-proliferation activity of this compound on human nasopharyngeal carcinoma cells CNE-1 and CNE-2, so there is no relevant report on the market. medicine

Method used

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  • Penicillium citrinum-derived penicitrinine A as well as application thereof to preparation of drugs for resisting nasopharyngeal carcinoma
  • Penicillium citrinum-derived penicitrinine A as well as application thereof to preparation of drugs for resisting nasopharyngeal carcinoma
  • Penicillium citrinum-derived penicitrinine A as well as application thereof to preparation of drugs for resisting nasopharyngeal carcinoma

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Experimental program
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Effect test

Embodiment 1

[0019] Fermentative production and separation and purification of the compound of embodiment 1

[0020] 1 Fermentation production

[0021] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Penicillium citrinum ( Penicillium citrinum ) IBPT-5 (preserved in the China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, and the deposit number is: CCTCCNO: M2013713) was inoculated onto PDA solid slant medium in a 28°C incubator Cultured for 4 days.

[0022] Get the Penicillium citrinum ( Penicillium citrinum ) IBPT-5 was inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, NaCl30.0 constant volume] in a 1000mL Erlenmeyer flask, after static culture at 28°C for 30 days, mycelium and fermentation broth were obtained.

[0023] 2 Obtaining th...

Embodiment 2

[0030] The test of embodiment 2 in vitro antitumor activity

[0031] 1 Experimental samples and experimental methods

[0032] Preparation of the tested sample solution The test sample is the pure product of the compound separated and purified in the above-mentioned implementation 1. Precisely weigh an appropriate amount of sample, and prepare a solution with the required concentration with DMSO for activity measurement.

[0033] The subculture of cell lines and cells adopts tumor cell lines, and the tumor cells are treated with RPMI1640 medium containing 10% FBS at 37°C in 5% CO 2 Subculture in an incubator.

[0034] Cell Proliferation Inhibitory Activity Test Method

[0035] The tumor cells in the logarithmic growth phase were collected by WST-1 method, and the cell density was adjusted to 5×10 per ml. 4 Cells were inoculated into 96-well cell culture plates at 100 μL per well, and placed in 5% CO at 37°C. 2 cultured overnight in an incubator. Aspirate the supernatant, ...

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Abstract

The invention relates to a preparation method and application of penicillium citrinum-derived penicitrinine A. The compound has the effect of restraining human nasopharyngeal carcinoma cell proliferation. The structural formula of the compound is shown in the description. According to the invention, through fermenting culture of penicillium citrinum IBPT-5, a fermentation product is obtained, and then the compound is separated and purified from the fermentation product. Experiment results confirm that the compound has better antitumor activity on human nasopharyngeal carcinoma cells such as CNE-1 and CNE-2. The penicillium citrinum-derived penicitrinine A can be applied to preparation of human nasopharyngeal carcinoma cell proliferation inhibition drugs or antitumor drugs to be used for research on human nasopharyngeal carcinoma.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a preparation method of penicitrinine A derived from Penicillium citrinus and its application in inhibiting the proliferation of human nasopharyngeal carcinoma cells. Background technique [0002] Alkaloids are a type of nitrogen-containing organic compounds produced by biological secondary metabolism. There are many types of alkaloids in nature, most of which come from plants, so they are also called plant alkaloids. Alkaloids have important physiological effects on humans and animals, including anti-asthma and antitussive, hypoglycemic, hypolipidemic, antibacterial, antitumor, analgesic, etc., among which antibacterial and antitumor activities are the most prominent. Natural structural alkaloids are an important source for discovering lead compounds in the research of innovative drugs, and there are nearly a hundred kinds of alkaloid drugs currently used in clinical practice...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/20C12P17/18A61K31/407A61P35/00C12R1/80
CPCC07D491/20C12P17/182
Inventor 郑秋红刘沁颖陈立应敏刚周彤
Owner 福建省肿瘤医院
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