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Applications of 4-4' isomerized secalonic acid D derived from Penicillium oxalicum in colon cancer

A technology of ryeketonic acid and penicillium oxalate, which is applied in the directions of medical preparations, digestive systems, and pharmaceutical formulations containing active ingredients to achieve the effect of inhibiting the proliferation activity of human colon cancer cells

Inactive Publication Date: 2019-01-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the indicated rymelic acid compound has anti-human colon cancer activity, and there is no report on the anti-proliferation activity of this compound on human colon cancer cells, so there is no drug related to it in the market.

Method used

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  • Applications of 4-4' isomerized secalonic acid D derived from Penicillium oxalicum in colon cancer
  • Applications of 4-4' isomerized secalonic acid D derived from Penicillium oxalicum in colon cancer
  • Applications of 4-4' isomerized secalonic acid D derived from Penicillium oxalicum in colon cancer

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Fermentative production and separation and purification of the compound of embodiment 1

[0019] 1 Fermentation production

[0020] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Penicillium oxalicum ( Penicillium oxalicum ) IBPT-6 (preserved in China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number is: CCTCC NO: M 2013714) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 2 to 3 days.

[0021] Penicillium oxalicum ( Penicillium oxalicum ) Appropriate amount of IBPT-6, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 ·7H 2 O 0.3, NaCl 15.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, the mycelia and fermentation broth wer...

Embodiment 2

[0029] Example 2 Test of anti-tumor activity in vitro

[0030] 1 Experimental samples and experimental methods

[0031] Preparation of the test sample solution: the test sample is the pure product of the compound separated and purified in the above-mentioned implementation 1. Precisely weigh an appropriate amount of sample, and prepare a solution with the required concentration with DMSO for activity measurement.

[0032] Take out the frozen cell line from the -80°C ultra-low temperature refrigerator or liquid nitrogen, dissolve it quickly in a 37°C water bath, centrifuge at 1000 rpm for 5 minutes, suck the frozen liquid in the ultra-clean bench, add 1 mL of medium and blow it Aspirate into the culture bottle, add 10 ml of fresh DMEM or RPMI 1640 medium, shake gently to suspend the cells evenly in the medium, store at 37°C, 5% CO 2 cultured in an incubator. When the cells are full and need to be subcultured, first discard the old medium in the bottle, then wash it twice wit...

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Abstract

The invention discloses applications of 4-4' isomerized secalonic acid D derived from Penicillium oxalicum in colon cancer. According to the present invention, the compound isomerization is rare 4-4'linkage and is different from the 2-2' linkage of the known secalonic acid D; and the experiment results prove that the polyphenol-based compound has good inhibitory activity against colon cancer cells, can be used for preparing colon cancer cell proliferation inhibiting drugs or anti-colon cancer drugs, and can be used for antitumor research.

Description

technical field [0001] The invention relates to the application of 4-4' isomerized ryeconic acid D derived from Penicillium oxalicum in colon cancer. Background technique [0002] Rhyronic acid is a kind of polyphenolic organic compound produced by the secondary metabolism of microorganisms. It has been more than half a century since ryronic acid A was first discovered from fungi in 1952. Since the discovery of this type of compound, a total of 9 compounds have been reported, namely ryronic acid A-I. With the exception of ryronic acid I, all ryronic acids of natural origin are 2-2' linked. Rhyronic acid I has a 4-2' connection, and a 4-4' connection is very rare. The compounds have obvious inhibitory effects on tumor cells and are ideal raw materials for developing anti-tumor drugs or tumor cell proliferation inhibitors. [0003] The inventors have studied and learned that Penicillium oxalicum ( Penicillium oxalicum ) IBPT-6, (preserved in China Center for Type Culture C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P35/00A61P1/00
CPCA61K31/352
Inventor 陈立王思远刘沁颖伍久林鲁志浩李亚平张其清
Owner FUZHOU UNIV
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