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Aspergillus versicolor and application thereof in preparing podophyllotoxin mono-glucoside and podophyllotoxin

A monoglucoside and podophyllotoxin technology is applied to a strain of Aspergillus versicolor and its application field in the preparation of podophyllotoxin monoglucoside and podophyllotoxin, which can solve the problem of uneconomical, no finding, and replacement of wild podophyllotoxins and other problems, to achieve the effects of low cost, short production cycle, and protection of plant ecological diversity

Active Publication Date: 2014-05-28
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] A lot of research has been done on the source of podophyllotoxin at home and abroad, however, no suitable way has been found to replace the wild podophyllum plant excavation
Podophyllotoxin drugs can be successfully obtained by total synthesis and semi-synthesis methods, but the total synthesis goes through too many steps, and there are too many isomers of podophyllotoxin obtained, so it is very uneconomical, while semi-synthesis requires natural podophyllotoxin as raw material

Method used

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  • Aspergillus versicolor and application thereof in preparing podophyllotoxin mono-glucoside and podophyllotoxin
  • Aspergillus versicolor and application thereof in preparing podophyllotoxin mono-glucoside and podophyllotoxin
  • Aspergillus versicolor and application thereof in preparing podophyllotoxin mono-glucoside and podophyllotoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, isolation and identification of bacterial strain

[0023] 1. Isolation of strains

[0024] The roots and rhizomes of the plant Diphyllria sinensis L. collected from the Red River Valley (1560m above sea level) in Meixian County, Shaanxi Province were washed with tap water and washed with 75% (volume percentage) Treat with ethanol aqueous solution for 5 minutes, then rinse with sterile water for 3-5 times; then treat with 2.5% (mass percentage) sodium hypochlorite aqueous solution for 10 minutes, and then rinse with sterile water for 3-5 times; Peel off the outer skin of the root of Erqi, cut into small pieces of 0.5cm×0.5cm and plant them on PDA solid medium, and cultivate them at 28°C for 3-7 days; After peeling and cutting, rolling on the PDA medium for one week, culture under the same conditions as a control observation.

[0025] After a few days of cultivation, it was found that mycelium grew from the incision of the root of Woerqi. Take the newly gr...

Embodiment 2

[0031] Example 2, Utilizing Aspergillus versicolor W1 to prepare podophyllotoxin monoglucoside and podophyllotoxin

[0032] 1. Cultivation of Aspergillus versicolor W1

[0033] The composition of the potato glucose liquid medium is: 200 g of peeled potatoes, 20 g of glucose, and add water to make the volume to 1000 mL. The preparation method of potato dextrose liquid medium: peel the potatoes and cut them into about 2cm 2 put into a beaker and boil for 30 minutes, then filter with double-layer gauze, take the filtrate and add glucose, then make up water, the pH is natural. Put 100ml of potato dextrose liquid medium in a 250ml Erlenmeyer flask, and sterilize it for later use.

[0034] A single colony of Aspergillus versicolor W1 was inoculated into 100ml potato dextrose liquid medium, and cultured with shaking at 28°C and 120r / min for 7 days.

[0035] 2. Preparation of crude extract

[0036] 1. Take the fermentation system obtained in step 1, use a suction filter to separat...

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Abstract

The invention discloses aspergillus versicolor and the application thereof in preparing podophyllotoxin mono-glucoside and podophyllotoxin. The preservation number of the aspergillus versicolor W1 is CGMCC NO.8571. The invention further provides application of the aspergillus versicolor W1 in preparing the podophyllotoxin mono-glucoside and / or the podophyllotoxin. The invention also provides a method for preparing the podophyllotoxin mono-glucoside and / or the podophyllotoxin. The method comprises the following steps: fermenting and culturing the aspergillus versicolor W1 so as to obtain the podophyllotoxin mono-glucoside and / or the podophyllotoxin. The podophyllotoxin mono-glucoside and the podophyllotoxin can be obtained after fermenting and culturing the aspergillus versicolor disclosed by the invention, the operation is simple, the cost is low, the production cycle is short, and the application potential for industrialized large-scale production is achieved. The invention provides a new way for the resource development of podophyllotoxin substances, and an important significance is realized in protection for the ecological diversity of plants.

Description

technical field [0001] The invention relates to a strain of Aspergillus versicolor and its application in preparing podophyllotoxin monoglucoside and podophyllotoxin. Background technique [0002] Podophyllotoxin (podophyllotoxin) and its derivative podophyllotoxin monoglycoside (podophyllotoxin7'-O-β-D-glucopyranoside) have a wide range of biological activities, especially its anti-tumor activity. Podophyllum plant extracts were included in the United States Pharmacopoeia as a natural medicine in 1820, and the main active ingredient is podophyllotoxin. Broad-spectrum antineoplastic drugs currently widely used in clinical practice, etoposide (etoposide), teniposide (teniposide), etoposide phosphate (etoposide phosphate) are all podophyllotoxin glycoside derivatives. [0003] Podophyllotoxin is a lignan isolated from podophyllum scutellaria resin. At present, podophyllotoxins mainly come from wild podophyllum plants, such as Podophyllum peltatum L, Sinopodophyllum emodi, an...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/60C12P17/18C12R1/66
Inventor 赵长琦梁子臻王珂贾栗
Owner BEIJING NORMAL UNIVERSITY
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