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A gibberella strain and its application in the preparation of rutin and quercetin

A technology of Gibberella and quercetin, which is applied to a Gibberella strain and its application field in the preparation of rutin and quercetin, can solve the problems of insufficient resources of Sophora japonica, high cost, and many by-products of hydrolysis, and achieves The effect of protecting plant ecological diversity, short production cycle and low cost

Active Publication Date: 2015-07-29
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, my country's rutin production mainly uses Huai Mi as raw material for extraction, but Huai Mi cannot fully meet the market demand due to insufficient resources. Therefore, it is of great significance to explore new sources of rutin to relieve the tight supply.
Traditional rutin extraction methods mainly include (hot) alkali extraction acid precipitation method, macroporous adsorption resin purification method, ultrasonic radiation extraction multiple recrystallization method, continuous reflux extraction method, etc. Although these traditional methods can successfully extract rutin, but There are various disadvantages, such as low yield, more hydrolysis by-products, complex process, higher cost, etc.

Method used

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  • A gibberella strain and its application in the preparation of rutin and quercetin
  • A gibberella strain and its application in the preparation of rutin and quercetin
  • A gibberella strain and its application in the preparation of rutin and quercetin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the separation and identification of Gibberella TG1

[0021] 1. Isolation of strains

[0022] The roots and rhizomes of Sinopodophyllum hexandrum (Royle) Ying collected from Xinglong Mountain (2300m above sea level) in Lanzhou City, Gansu Province were washed with tap water and placed in an ultra-clean workbench. Treat with 75% (volume percent) ethanol aqueous solution for 5 minutes, then rinse with sterile water for 3-5 times; then treat with 2.5% (mass percent) sodium hypochlorite aqueous solution for 10 minutes, and then rinse with sterile water for 3-5 times; Use sterilized tweezers and blades to peel off the outer skin of the root of Taoerqi, then cut into small pieces of 0.5cm×0.5cm and plant them on PDA solid medium, and cultivate them at 28°C for 3-7 days; at the same time, sterilize the same The roots of the treated Daoerqi were not peeled and cut, rolled on the PDA medium for one week, and cultured under the same conditions were observed as a co...

Embodiment 2

[0029] Embodiment 2, utilize Gibberella TG1 to prepare rutin and quercetin

[0030] 1. Cultivation of Gibberella TG1

[0031] The composition of the potato glucose liquid medium is: 200 g of peeled potatoes, 20 g of glucose, and add water to make the volume to 1000 mL. The preparation method of potato dextrose liquid medium: peel the potatoes and cut them into about 2cm 2 put into a beaker and boil for 30 minutes, then filter with double-layer gauze, take the filtrate and add glucose, then make up water, the pH is natural. Put 100ml of potato dextrose liquid medium in a 250ml Erlenmeyer flask, and sterilize it for later use.

[0032] A single colony of Gibberella TG1 was inoculated into 100ml of potato dextrose liquid medium, and cultured with shaking at 28°C and 120r / min for 7 days.

[0033] 2. Preparation of crude extract

[0034] 1. Take the fermentation system obtained in step 1, use a suction filter to separate and collect the fermentation supernatant.

[0035] 2. Ta...

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Abstract

The invention discloses a strain of gibberella and the application thereof in preparing rutin and quercetin. The preservation number of gibberolla intermedia TG1 is CGMCC NO.8572. The invention further comprises the application of the gibberolla intermedia TG1 in preparing the rutin and / or the quercetin. The invention also comprises a method for preparing the rutin and / or the quercetin. The method comprises the steps of fermenting and culturing the gibberolla intermedia TG1 to obtain the rutin and / or the quercetin. The endogenous gibberella provided by the invention can be used for preparing the rutin and the quercetin via fermentation, so that the operation is simple, the cost is low, the production cycle is short, and the application potential for industrialized large-scale production is achieved. According to the invention, a new way for the resource development of the rutin and the quercetin is provided, and an important significance is realized in protecting the ecological diversity of plants.

Description

technical field [0001] The invention relates to a gibberella strain and its application in preparing rutin and quercetin. Background technique [0002] Rutin, also known as rutin, is one of the main forms of quercetin, a class of flavonoids with a wide range of sources, mainly found in the flower buds of Sophora japonica L. (Sophora japonica L.) , fruit (Sophora japonica), Rutaceae plant Ruta (Ruta graveolenslens L.) whole plant, Hypericum plant red eclipse (Hypericum ascyron L.) plant and Polygonaceae plant buckwheat (Fagopyrum esculentum Moench) seedlings . [0003] Rutin has a wide range of utilization value, which is mainly reflected in its use as a therapeutic drug and health care product. Rutin has vitamin P-like effects, can reduce capillary permeability and fragility, promote cell proliferation and prevent blood cell aggregation, and have anti-inflammatory, anti-allergic, diuretic, antispasmodic, antitussive, and blood lipid-lowering effects. The pharmaceutical in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/60C12P17/06C12R1/645
Inventor 赵长琦王珂张嘉梁子臻贾栗
Owner BEIJING NORMAL UNIVERSITY
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