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Xanthone compounds and preparation method thereof, and application of Xanthone compounds in field of agriculture

A technology of compounds and mixtures, applied in the field of fungal extracts, to achieve good inhibitory effect and excellent herbicidal performance

Active Publication Date: 2020-11-17
TOBACCO RES INST CHIN AGRI SCI ACAD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that microbial metabolites derived from marine fungi have medicinal effects, such as anti-tumor and anti-inflammatory activities, but there are few reports on their agricultural biological activities. Therefore, attempts to develop them from secondary metabolites of marine fungi Its biopesticide potential is the focus of research by personnel in this field

Method used

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  • Xanthone compounds and preparation method thereof, and application of Xanthone compounds in field of agriculture
  • Xanthone compounds and preparation method thereof, and application of Xanthone compounds in field of agriculture
  • Xanthone compounds and preparation method thereof, and application of Xanthone compounds in field of agriculture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 strain source

[0033] The embodiment of the present invention provides a herb-inhibiting fungus screened from seaweed. The herb-inhibiting fungus is Aspergillus versicolor D5. The date is February 6, 2018, and the preservation address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0034] The Aspergillus versicolor D5 was cultured on a PDA plate medium at 28° C. for 5 days, the diameter of the colony was 3 cm, the hyphae were orange-yellow, the surface was flocculent, and the edge was white. The conidia heads are radial at the beginning, the apical capsule is slightly elongated or slightly oval, the sporulation structure is double-layered, and the conidia are balls.

[0035] The 18S rDNA gene sequence of the Aspergillus versicolor D5 strain has been submitted to the GenBank database with the accession number MG827180 (since the gene sequence has been submitted to the GenBank datab...

Embodiment 2

[0036] Embodiment 2 fermentation culture obtains extract

[0037] Aspergillus versicolor D5 strain was grown on potato dextrose agar medium (PDA) for one week, edge hyphae were picked and added to the seed medium, and cultured at 28°C and 180rpm for 72h with shaking. Add 400mL potato dextrose water culture medium into a 1000mL fermentation bottle, after sterilization, absorb 5mL seed solution and add it to the culture medium, let it stand at 28°C for 30 days, and ferment 100L in total. It can be understood that the fermentation temperature of the strain can be around 28°C, and the specific fermentation time can be shortened or extended according to the fermentation situation.

[0038] Among them, potato dextrose agar (PDA) medium: potato extract powder 5-6g / L, glucose 15-20g / L, coarse sea salt 25-30g / L, and the rest is deionized water. It can be understood that the ratio of potato and glucose in the medium is optimized and selected for Aspergillus versicolor D5, which can eff...

Embodiment 3

[0041] Example 3 compound separation

[0042] The obtained fermented extract is processed sequentially by using ethyl acetate-petroleum ether solvent system (the elution ratio of ethyl acetate is 0-100%) and methanol-ethyl acetate solvent system (the elution ratio of methanol is 0-50%) The normal phase decompression column chromatography separated to obtain seven components of components 1-7.

[0043] Gradient elution of reversed-phase silica gel column chromatography was performed on component 2, and the solvent was 50%-100% methanol-water to obtain component 2-1-component 2-5. Component 2-2 was purified by Sephadex LH-20 gel column chromatography (dichloromethane / methanol, v / v, 1:1), and then purified by high performance liquid chromatography (HPLC), the solvent was 65% methanol-water , to obtain compound 11. Components 2-3 were subjected to SephadexLH-20CC (dichloromethane / methanol, v / v, 1:1) gel column chromatography, and then ODS reversed-phase silica gel column chromat...

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Abstract

The invention provides Xanthone compounds and a preparation method thereof, and application of the Xanthone compounds in the field of agriculture, belonging to the field of fungus extracts. A fermentation extract is obtained from Aspergillus versicolor D5 derived from ocean, and 15 compounds are obtained through separation and extraction, wherein 6 compounds are identified as new compounds, and 9compounds are identified as known compounds. The resuts of herbicidal activity tests and antifungal activity tests show that the Xanthone compounds have good antifungal and herbicidal activity, are environment-friendly, safe and efficient, and have better or equivalent control effects compared with chemical pesticides.

Description

technical field [0001] The invention belongs to the field of fungal extracts, and in particular relates to a Xanthone compound, a preparation method thereof and an application in the agricultural field. Background technique [0002] Plant diseases and weeds seriously threaten the growth of crops and cause huge economic losses to agricultural production every year. For a long time, chemical pesticides have played an important role in the control of plant diseases and weeds, and are of great significance to improving crop quality and yield and reducing labor intensity, but they are prone to environmental pollution, pesticide residues and pathogenic weeds. Resistance and other negative effects. [0003] In order to implement the "Action Plan for Zero Growth in Pesticide Use by 2020" formulated by the Ministry of Agriculture and Rural Affairs, my country vigorously advocates the use of microbial pesticides. Currently, live microbial pesticides are the most used, but their disad...

Claims

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Application Information

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IPC IPC(8): C07D311/86C07D493/14C07D311/58C12P17/18C12P17/06A01N43/90A01N43/16A01P3/00A01P13/00C12R1/66
CPCC07D311/86C07D493/14C07D311/58C12P17/181C12P17/06A01N43/90A01N43/16Y02A50/30
Inventor 赵栋霖韩小斌张成省刘京李义强彭玉龙阳显斌温明霞王小彦罗玉英
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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