The invention relates to the field of detergents, in particular to laundry condensate beads for washing underclothes, and a preparation method of the laundry condensate beads. The laundry condensate beads for washing the underclothes are respectively prepared from a water-soluble film and a cleaning solution arranged therein, wherein the cleaning solution is prepared from the following components: propylene glycol, sorbitol, glycerol, dodecyl benzene sulfonic acid, ethanol amine, a non-ionic surface active agent, coconut oil fatty acid, a maleic acid / acrylic acid copolymer, a chelating agent, ethanol, a bactericide, quaternary ammonium salt organo-siloxane, deionized water, essence, a bittering agent and enzyme. According to the laundry condensate beads, the adding amount of an anionic surfactant is reduced, so that the irritation to the skin is reduced; the laundry condensate beads have a stronger killing effect on common pathogenic bacteria. A softener component is added into the laundry condensate beads, so that clothes are stiffened and not dry and hard after being washed by using the laundry condensate beads, and the comfort level of the clothes is improved.