202results about How to "Suitable for preservation" patented technology

The invention discloses a refrigerator and its temperature control method. The method comprises the following steps: detecting the current temperature of a variable-temperature chamber; judging whether a difference between a shutdown temperature of the variable-temperature chamber and the current temperature is not lower than a first difference temperature; if yes, detecting an ambient temperature of the refrigerator, and judging whether the ambient temperature is not higher than a preset ambient temperature; and if yes, controlling a temperature compensation heater in a first preset mode to heat the variable-temperature chamber, otherwise controlling the temperature compensation heater to operate in a second preset mode. The refrigerator temperature control method of the inventive embodiment can precisely control the temperature compensation heater to carry out temperature micro-adjustment on the variable-temperature chamber in different modes when the set temperature of the variable-temperature chamber is relatively high (usually used for a refrigerating chamber), to make the temperature of the variable-temperature chamber quickly reach the set temperature, and has advantage of high control accuracy, thus contributing to food storage in the variable-temperature chamber and improving practicability of the refrigerator.

The invention discloses a human umbilical cord mesenchymal stem cell (HUMSC) anti-hepatic fibrosis injection and a preparation method thereof. The injection is composed of 10%-50% of human albumin stock solution, 49.5%-89.5% of acetated Ringer's solution and 0.5% of heparin calcium, wherein, the concentration of the human albumin stock solution is 10%, and 1ml of the injection contains 6*10<5>-7*10<5> HUMSCs. The preparation method comprises the following steps: providing an MSC preserving fluid and pre-cooling the MSC preserving fluid for later use, wherein, the MSC preserving fluid comprises the human albumin stock solution, the Acetated Ringer's solution and the heparin calcium; providing the HUMSCs and adding the HUMSCs to the MSC preserving fluid; and adjusting the number of the HUMSCs added to the MSC preserving fluid to be 6*10<5>-7*10<5> HUMSCs / ml by means of re-suspending. The injection provided by the invention has the advantages of obvious curative effect and high stability, and is convenient in storage and transport and safe in use, thus being capable of bringing gospel to patients with hepatic fibrosis.

A hinge-lid type package for filter cigarettes is formed as a rectangular parallelepiped and includes an inner pack 36 of a cigarette bundle and a casing formed from a blank 50 as one sheet and accommodates the inner pack 36. The casing can be divided into an outer body 10 and a lid 12 by means of a separating line 14. When detached from the outer body 10 along the separating line 14, the lid 12 is rotated around a self hinge 16 to open or close.

The invention discloses a mesenchymal stem cell self-preserving liquid which is prepared from the following raw materials according to volume ratios: 1-6% of self platelet lysate, 95-97% of solution medium and 0-1% of vitamin C injection, wherein the solution medium is a mixed sugar electrolyte injection or physiological saline injection. The mesenchymal stem cell self-preserving liquid has the advantages that: growth factors contained in the mesenchymal stem cell self-preserving liquid can maintain the activity state of cells, the vitamin C can maintain the activity of various peroxidases, at the same time, the mixed sugar electrolyte injection is especially suitable for cell preservation, and osmotic pressure is also favorable for nutrient absorption of the cells so as to carry out metabolism; the activity of the mesenchymal stem cells which are preserved in the mesenchymal stem cell self-preserving liquid within 8 hours is still larger than 90%, the cell activity change is not larger than 7%, and the activity of the mesenchymal stem cells which are preserved in the mesenchymal stem cell self-preserving liquid within 12 hours is still maintained at 85%; and by using the mesenchymal stem cell self-preserving liquid, the requirements for human intravenous back-transfusion safety can be met, and the cell preserving problem of long-time transport is solved, thus more patients have opportunity to undergo mesenchymal stem cell treatment.

The present invention relates to methods for screening of cellular responses of cellular components comprising: (a) providing cellular components on the surface of a substrate, said substrate having immobilized thereon an array of detector molecules; (b) delivering test compounds to positions on the substrate corresponding to the arrayed detector molecules on the surface of said solid substrate; (c) incubating said test compounds with said cellular components on the surface of the solid support, under conditions allowing the induction of cellular responses; (d) assaying said cellular responses; and, identifying and characterizing the cellular responses induced by said test compounds. The present invention further relates to the uses of said methods as well as microarrays and kits for carrying out said methods.

The invention discloses a preparation method of a controlled release antibacterial film. The method includes the following steps of: (1) dissolving zein and a hydrophobic antibacterial agent into an alcoholic solution; (2) preparing a sodium caseinate aqueous solution; (3) adding the sodium caseinate aqueous solution into the solution obtained in step (1) under stirring; (4) subjecting the solution obtained in step (3) to rotary evaporation and centrifugation, then freeze drying the supernatant, thus obtaining nanoparticle powder; (5) dissolving a film-forming matrix in water, adding the nanoparticle powder and lysozyme to form a mixed solution; and (6) adding glycerin, and conducting film pouring, thus obtaining the film product. The method provided in the invention can simultaneously reduce initial microbial activity and inhibit microbial growth for a long term, and has the advantages of low instrument requirement, and controllable bacterium inhibiting intensity, thus having broad application prospects in food and medical products.

The invention discloses a moisture-proof deoxidizing agent for foodstuff, and belongs to the technical field of a deoxidizing agent for foodstuff. The moisture-proof deoxidizing agent for the foodstuff disclosed by the invention comprises the following components in parts by weight: 90-110 parts of iron powder, 5-15 parts of calcium chloride, 5-15 parts of activated carbon and 20-40 parts of auxiliary material, wherein the auxiliary material is attapulgite and montmorillonite, and the mass ratio is (4-6) to (4-6). The moisture-proof deoxidizing agent for the foodstuff disclosed by the invention has a long-term deoxygenization effect, and the antioxidant effect of an easy-to-oxidize food can be significantly improved. Meanwhile, the original oxygen absorbing performance still can be kept under a high-humidity condition, and the moisture-proof deoxidizing agent also has an auxiliary dehumidifying effect, and is significant in effect, simple in preparation technology, convenient to add and use, high in safety, lasting in action, low in production cost, applicable to preservation of various foods, and beneficial to popularization and application.

The invention provides methods for enriching methyl-CpG sequences from a DNA sample. The method makes use of conversion of cytosine residues to uracil under conditions in which methyl-cytosine residues are preserved. Additional methods of the invention enable to preservation of the context of me-CpG dinucleotides. The invention also provides a recombinant, full length and substantially pure McrA protein (rMcrA) for binding and isolation of DNA fragments containing the sequence 5′-CMeCpGG-3′. Methods for making and using the rMcrA protein, and derivatives thereof are provided.

The invention belongs to the field of health food processing, and particularly relates to a preparation method of leisure natto. The preparation method is characterized by comprising the following steps: carefully selecting, cleaning, soaking, stewing and cooling soybeans; inoculating the soybeans with bacillus natto, and fermenting; soaking the fermented natto into a sauce, enabling the whole fermented natto to be submerged in the sauce, and refrigerating the natto for 12-24h at 2-8 DEG C; taking out the natto after the natto is tasty, and draining out surface water; air-drying the natto at 30-50 DEG C, wherein the salinity of the air-dried leisure natto is 0.5-3% and the water content of the air-dried leisure natto is 10-16%. The invention aims to overcome the defects that the existing natto has strong ammonia odor and a large amount of mucilage, is not good-looking, affects appetite, is inconvenient to store and carry, and cannot be eaten at any time to prepare the leisure natto which is suitable for Chinese people, convenient to store and carry, can be eaten at any time, and has bioactivity, so as to enable more Chinese people to enjoy the delicious, nutritional and healthy leisure natto.

The invention relates to an aerobic nitration granule sludge long-term storage and activity recovery method, belonging to the technical field of sludge granule storage and recovery. The method comprises the following steps: 1. determining the COD (chemical oxygen demand) and ammonia nitrogen removal capacity of the aerobic granule sludge before storage; 2. carrying out freeze-drying on the pretreated aerobic granule sludge, and storing; and 3. recovering activity of the stored granule sludge, and determining the COD and ammonia nitrogen removal capacity. The method can solve the problem of long activity recovery period of the nitrifying bacteria in the aerobic granule sludge after long-term storage.

The invention belongs to the field of preparation methods of organism specimens, relates to an aquatic organism specimen and particularly relates to a novel method for storing an aquatic organism specimen by using epoxy resin. The method comprises the following effective steps of (a) dehydration, (b) resin preparation; (c) embedding; and (d) solidification. By using mixed preparation of the epoxy resin and a curing agent, the whole aquatic organism specimen is simple and fast in preparation and low in cost; the specimen produced by using the method can adapt to the research requirements of an organism teaching tool and an organism specimen of a standard sample. Meanwhile, the organism specimen prepared through the method is convenient to store, transport, exchange and communicate, the classification and identification characteristics of microorganisms are well stored, and the organism specimen is not easy to age and is especially suitable for storage of small and miniature aquatic organisms.

The invention provides a preparation method of a polyethyleneglycol monomethyl ether-polylactic acid block copolymer, which comprises the following steps: reacting raw materials polyethyleneglycol monomethyl ether and D,L-lactide by melt polycondensation by using stannous octoate as a catalyst; and after the reaction finishes, carrying out after-treatment by a 1-4-time dichloromethane dissolution-aether crystallization process, wherein the product to be purified is dissolved by the dichloromethane and washed with pure water in the first or second crystallization process to remove more low-molecular impurities, thereby obviously narrowing the molecular weight distribution of the product. The hydroscopic property of the product is obviously improved, and the flowability is higher and is more suitable for preservation and use of the product.

The invention belongs to the technical field of seed preservation and relates to a Dendrobium officinale seed preservation device and method. The device comprises a storage box and seed collection bottles; the seed collection bottles mounted in the storage box are used for storing Dendrobium officinale seeds. The Dendrobium officinale seeds are collected in the seed collection bottles. After mouths of the seed collection bottles are covered with bottle caps, and the outer surface, connected with the bottle cap, of each mouth is provided with a breathable seal film; the seed collection bottles are mounted in holes of bottle racks. The method includes: sterilizing tools, sterilizing capsules, collecting seeds and preserving at low temperature. The Dendrobium officinale seed preservation device and method has the advantages that the device is reasonable in structural design, the method is scientific and reliable, preservation time is long, survival rate is high, the requirements of Dendrobium officinale for perennial tissue culture can be met, and the device and method is applicable to collection and storage of like seeds.

The invention discloses a preparation method of a superhigh-temperature aerobic fermentation microbial agent prepared from municipal sludge. The preparation method comprises steps as follows: superhigh-temperature aerobic bacteria are activated and then fermented, a supernatant is removed from fermentation broth, a protective agent is added and is stirred and mixed uniformly, the mixture is driedand ground by a pulverizer, and a product is screened and subpackaged. The scheme has the advantages as follows: firstly, the low-cost municipal sludge is taken as a main fermentation raw material forregulation and preparation of a fermentation medium to realize fermentation production of the microbial agent, one idea is provided for solving the problem of pollution caused by the municipal sludge, protection and improvement of the ecological environment are facilitated, fermentation cost of the superhigh-temperature aerobic bacteria is reduced, and fermentation efficiency is improved; secondly, compared with a fermentation microbial agent prepared with a conventional method, survival rate and storage period of the superhigh-temperature aerobic fermentation microbial agent can be effectively increased with addition of a composite support and the protective agent, and the microbial agent is suitable for long-term storage; finally, the application cost of the superhigh-temperature aerobic bacteria is reduced, and the microbial agent and the preparation method are suitable for large-scale industrial production and application.

The invention discloses a preparation method of intelligent surface mosquito-repellent polyester fiber for a mosquito net. The preparation method comprises the following steps: performing etching reduction treatment on polyester fiber; preparing mosquito-repellent polyester fiber from a mosquito repellent by a block copolymer disperse coating method so that traditional polyester fiber becomes a high-performance spinning intelligent material and has different responsiveness to different chemical environments to finally realize a lasting mosquito killing effect of polyester filament. The mosquito-repellent polyester fiber for the mosquito net prepared by the method disclosed by the invention is scientific and technological polyester chemical fiber filament, and the mosquito repellent is uniformly distributed in the fiber surface; the mosquito-repellent polyester fiber offers different intelligent responses under the effect of specific auxiliaries and can be stored and used for long time; and moreover, the mosquito-repellent polyester fiber has the advantages of high mosquito-repellent efficiency, long duration and low toxicity and is relatively widely applicable to house materials.

The invention discloses a method for making an earthtype tea set. The method comprises the following steps that: mottled earth, loess, blue earth and purple soil are respectively mixed according to the weight percent of 56 to 64 percent, 17 to 23 percent, 4 to 6 percent and 13 to 17 percent, and then are ground; water is added in to carry out stirring; slurry is calmed and filtered; sunshine is carried out; water is filtered out to form mud; billet drawing and finishing are finished; natural drying is carried out; oxidizing flame negative pressure baking is finished at 1,100 DEG C; equal to or higher than 1,110 DEG C and lower than or equal to 1,180 DEG C reducing flame positive pressure baking is carried out to generate kiln transformation; and finally, natural cooling, grinding and polishing are carried out. The earthtype tea set made by the method is firm and durable and has high drying degree, infrequent water absorption and better gas permeability after grinding and polishing; therefore, the earthtype tea set is suitable for containing tea and Pu'er tea particularly for a long time, and can prevent the tea from easy deterioration; moreover, the tea set has smooth and rounded surface, excellent hand feeling and both practicality and ornamental property.

The invention relates to a carboxymethyl chitosan co-mixed fresh-keeping agent and a film-coating fresh-keeping method for a fresh egg, and belongs to the egg product fresh-keeping field. The chicken egg fresh-keeping agent is obtained by mixing the following components in a pure water solution: carboxymethyl chitosan, glycerin and calcium chloride. The carboxymethyl chitosan co-mixed fresh-keeping agent coats the surface of a cleaned and dried egg by a solution-soaking method, and then natural air drying is carried out. The prepared fresh-keeping agent has the advantages of wide sources, no toxicity, no pollution, and biodegradability; and moreover, carboxymethyl chitosan has good bacteriostasis performance, and the preparation cost is low. The egg fresh-kept by the fresh-keeping agent still belongs to an A-grade egg after being placed for two months at the normal temperature. The method is also suitable for fresh keeping of other fowl eggs.

The invention provides a safe blood or blood component storage container and a preparation method thereof. The storage container comprises a storage bag and a tube; wherein, the storage bag is made of a polyurethane / polyvinyl chloride double-layer composite cylinder film; the tube is made of a polyurethane / polyvinyl chloride double-layer composite tube; the inner layer of the polyurethane / polyvinyl chloride double-layer composite cylinder film is made of polyurethane, and the corresponding outer layer thereof is polyvinyl chloride; the inner surface of the polyurethane / polyvinyl chloride double-layer composite tube is a polyurethane layer, and the outer surface thereof is a polyvinyl chloride layer. The polyurethane / polyvinyl chloride double-layer composite cylinder film is prepared by a co-extrusion blow molding method, and the polyurethane / polyvinyl chloride double-layer composite tube is prepared by a co-extrusion method. The storage container has excellent blood compatibility, can avoid micromolecules such as phthalate di (2- ethylhexyl), plasticizer and the like to pollute the blood, meets the requirements of humid hot sterilization on heat resistance, has the advantages of high mechanical strength, easy bonding and low manufacturing cost, and is suitable for high frequency soldering.

The invention provides a placenta and umbilical cord cell protective solution. The protective solution is mainly prepared by dissolving a mycoplasma inhibitor and aminoglycoside antibiotics with an aqueous solution of MEM-alpha medium. 1 mL of the aqueous solution of MEM-alpha medium is respectively used for dissolving 10-30 mg of the mycoplasma inhibitor and 180-240 U of the aminoglycoside antibiotics. Concentration of the aqueous solution of MEM-alpha medium is 10-15 mg / mL. The protective solution provided by the invention not only can effectively kill or inhibit formation of mycoplasma and prevent placenta cell or umbilical cord cell infection, but also can provide a stable osmotic pressure environment for placenta or umbilical cord cells through addition of protective components. Then, human environment can be effectively simulated, and cell survival rate is raised. Meanwhile, nutrients can be provided for storing and transporting cells within a short period of time; immune performance of cells is enhanced; cell infection is prevented; cell viability is effectively improved; and cell transport time is prolonged.

The invention provides six-delicacy filling for dumplings and a preparation method thereof. The three-delicacy filling for the dumplings comprises pig lean meat 20-30 parts, pig backfat 20-30 parts, dried shelled shrimps 2-4 parts, Chinese chives 4-8 parts, dried scallops 5-8 parts, sea cucumbers 8-12 parts, shrimp eggs 2-4 parts, clear water 12-15 parts, dried shelled shrimp water 2-4 parts and dried scallop water 3-5 parts. The six-delicacy filling for the dumplings has multiple seafood species and enough delicate flavor and is free of fishy smell.

The invention discloses a difunctional deoxidier capable of quickly dehumidifying and a preparation method thereof. The deoxidier capable of quickly dehumidifying contains deoxidizing components and dehumidifying components which respectively account for the deoxidier capable of quickly dehumidifying in percentage by mass: 32 percent-47 percent of iron powder, 0.03 percent-1 percent of auxiliaries, 3 percent-7 percent of catalyst, 9 percent-18 percent of filler, 0.3 percent-0.5 percent of sulfuric acid, 7 percnet-10 percent of water, and 30 percent-38 percent of dehumidifying components. The preparation method comprises the following steps: after mixing the catalyst, the suxiliaries, the concentrated sulfuric acid and the water in proportion, quickly stirring evenly; adding the iron powder and stirring continuously, so that the iron powder is activated to form a primary battery with metal in the auxiliaries; adding the filler, and stirring evenly to obtain the deoxidizing components; and finally, after sealing the deoxidizing components, and putting the deoxidizing components and the dehumidifying components in a sealing body for sealing to obtain the deoxidier capable of quickly dehumidifying. The difunctional deoxidier capable of quickly dehumidifying is suitable to be widely used in food storage and goods storage in other fields; and the preparation method has simple process, obvious effect and low cost.

A rodenticide with longer storage time without deterioration is proportionally prepared from brornadiolone, bittering agent (C28H34N2O3), edible alcohol, ethandiol as adhesive, carmine red 60 and rice grain.

The invention relates to a normal-temperature preservation method for denitrifying bacteria. The normal-temperature preservation method comprises the following steps: (1) culturing denitrifying bacteria to a logarithmic phase and collecting the bacteria; (2) preparing a preservation nutrient solution which mainly comprises a carbon source, a nitrogen source, trace substances, etc.; (3) mixing thedenitrifying bacteria obtained in the step (1) with the preservation nutrient solution obtained in the step (2) in a preservation container, and adding a proper amount of a preservation aid, wherein the preservation aid comprises glycolipid, sugar alcohol and organic acid salts; and (4) subjecting mixed liquor obtained in the step (3) to hermetic culture for 4 to 12 h and then carrying out normal-temperature preservation. Compared with the prior art, the method provided by the invention is applicable to normal-temperature long-term preservation of a great amount of denitrifying bacteria and has the advantages of good preservation effect at normal temperature, high survival rate, quick activity recovery, etc.

The invention discloses an animal feed producing method, comprising the following steps: 1) the heavy metal and fluoride contents of each part of a water hyacinth is measured, the water hyacinth being accordant with the national feed safety prescription is chosen; 2) the silage formed through the mixing of the stems and the leaves of the fresh water hyacinth which accords with the prescription and the wheat bran under a sealed condition, wherein, the wheat bran covers 8 percent to 12 percent of the silage gross weight. The method of the invention can convert the water hyacinth into silage which is full of nutrition to be effectively stored.

The invention discloses a real-time data storage device. The real-time data storage device comprises an interface layer, a caching layer, a column-type database layer and a data persistence layer, wherein the interface layer is used for providing a querying interface and a writing-in interface outwards, analyzing a querying order and a writing-in order to be in an internal-appointment data format, then transmitting the result to the caching layer, packaging the querying result and the writing-in result and carrying out responding; the caching layer is used for receiving the querying order and the writing-in order which come from the interface layer, and returning the querying result and the writing-in result to the interface layer; the column-type database layer is used for storing data indexes and data contents; the data persistence layer is used for storing file blocks through a distributed file system. The real-time data storage device has the advantages that the international standard of the field of the Internet of Things is achieved, and main application scenes of the field of the Internet of Things are met; particularly, for storage of a larger amount of sensor data, a single-point fault is avoided, and the reliability is high while the capacity is enormous.

The invention belongs to the technical field of food processing and discloses nutrition-balanced instant health-preserving rice and a preparation method thereof. The health-preserving rice is prepared from the following raw materials in percentage by mass: 20-25% of rice, 3-8% of millets, 3-8% of soybeans, 5-8% of corn grains, 5% of Chinese yams, 5% of coix seeds, 5% of wheat, 5% of bearded tooth mushroom, 5% of carrots, 5% of cassava, 4% of euryale ferox seeds, 3% of kudzuvine roots, 2% of poria cocos, 2% of walnut kernels, 1% of dried orange peels and 20-23% of water. The preparation method of the nutrition-balanced instant health-preserving rice comprises the following steps: crushing the raw materials; mixing the crushed materials and conditioning the mixed materials; carrying out extruding so as to granulate the conditioned material; carrying out baking so as to shape the granules; and cooling the shaped granules and packaging the cooled granules. The health-preserving rice satisfies the basic requirements of 'diversified foods on basis of cereals' in the Dietary Guidelines for Chinese Residents, and is free of addition of any chemical additives. The health-preserving rice can be eaten after being brewed with hot water of which the temperature is 80 DEG C or above for three minutes; and the brewed health-preserving rice is similar to a rice porridge, and is golden in color and luster, refreshing in taste and mutually complementary in nutrients. The nutrition-balanced instant health-preserving rice is a meal-replacement food suitable for modern sub-healthy populations, and has wide market prospects and promotion values.

The invention provides skin spermatogonium protection liquid and a preparation method thereof. The protection liquid is mainly formed by dissolving aminoglycoside antibiotics in DMEM (Dulbecco's modified eagle medium) culture medium water solution, 80-120U of the aminoglycoside antibiotics are dissolved in every mL of the DMEM culture medium water solution, and the concentration of the DMEM culture medium water solution is 13-25mg / mL. The skin spermatogonial stem cell protection liquid sufficiently simulates a human internal environment and is more suitable for survival of skin stem cells and spermatogonial stem cells and stable in property, the survival rate of the skin stem cells and the spermatogonial stem cells can be remarkably improved, and cultivation of the skin stem cells and the spermatogonial stem cells can be further enlarged while the skin stem cells and the spermatogonial stem cells are preserved.