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A kind of Amanita liposa strain yx1, culture method and application thereof

The technology of a fatty scale umbrella and a cultivation method, which is applied in the field of edible fungi, can solve problems such as water quality changes, waste of reagents, and high labor intensity of operators, and achieve the effects of increasing mycelium biomass, reducing costs, and benefiting ecological environment protection

Active Publication Date: 2022-01-07
SHANDONG PENGBO BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium citrate and sodium acetate are added to the medium during use, and the yield of Agaricus bisporus is increased by spraying sodium phosphate and potassium phosphate. The labor intensity of the operator is high, and most of the reagents are wasted during the spraying process, which may easily cause changes in water quality.

Method used

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  • A kind of Amanita liposa strain yx1, culture method and application thereof
  • A kind of Amanita liposa strain yx1, culture method and application thereof
  • A kind of Amanita liposa strain yx1, culture method and application thereof

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Effect test

Embodiment 1

[0039] Embodiment 1: a kind of Amanita liposa strain ( Pholiota adiposa ) YX1

[0040] The strain is collected from the wild forest of Mount Tai. The original strain is obtained through isolation, purification, and cultivation. After domestication, hybridization, breeding, and identification in the laboratory, a strain with powerful medicinal functions and good taste is obtained. sex and scarcity.

[0041] 1. Isolation and screening of strains:

[0042] Select fresh wild Amanita spp. with neat flower shape, large fruiting bodies, and use conventional tissue separation methods (rinse the fruiting bodies with running water, dry the water with filter paper, rinse 3 times with sterile water, tear apart mushroom umbrella, take out the white mushroom meat and cut out small pieces, rinse with sterile water 3 times) to obtain sterile tissue blocks. The tissue block was inoculated on the mother seed medium after high temperature and high pressure sterilization at 121°C for 30 minute...

Embodiment 2

[0055] Embodiment 2: the culture method of Amanita liposa strain YX1

[0056] Step 1, mother species cultivation:

[0057] Inoculate the sterile tissue block on the sterilized mother seed medium and culture it in a biochemical incubator at a temperature of 20-25°C to obtain the bacterial block.

[0058] Mother seed medium formula: 150~250g potato, 15~25g glucose, 10~20g yeast extract, 1~3g magnesium sulfate, 1~3g potassium dihydrogen phosphate, 0.3~0.5mg vitamin C, 15~20g agar, water 1000ml. Adjust the pH to 5.5~6.5 and sterilize.

[0059] This formula can make mycelia grow more slender, and the number of cells per unit volume of mycelium is relatively large, which provides conditions for liquid culture in the future.

[0060] Step 2, original seed cultivation:

[0061] Inoculate the bacterial block obtained in step 1 into the original seed medium, place it in an incubator at a temperature of 20-25°C and cultivate it for 4-5 days, and shake the bacteria at a speed of 140-1...

Embodiment 3

[0073] Embodiment 3: Agaricum multiliposa YX1 fruiting body transformation product

[0074] 3.1 Drying products:

[0075] Collect fresh fruit bodies and place them in a dryer at a temperature of 30-35°C for 2-4 hours, then gradually raise the temperature to 40-60°C for 10-14 hours, and bake at 60-65°C 1~2 hours. Dried products are convenient for storage, transportation and sales. Soak the dried fruiting bodies in warm water at 35-40°C for 3-4 hours before eating. The fat-scaled umbrella fruiting bodies maintain the smooth and tender flesh, strong fragrance, and bright color of fresh mushrooms. It can be described as a school of its own among edible mushroom varieties.

[0076] 3.2 Powdered health care products: After drying, the fruiting bodies are ground into powdered products of 100~300 mesh by a 25,000~30,000r / min grinder. Rich in polysaccharides, amino acids, and sterols, it can be ground into powder to extract polysaccharides and other products. The detection results...

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Abstract

The invention discloses a strain of Agaricus polyphagoides ( Pholiota Adiposa ) YX1, cultivation method and its application belong to the technical field of edible fungus. The lipopolyphaga YX1 has been deposited in the General Microorganism Center of China Microbiological Culture Collection Management Committee on December 1, 2020, and the strain preservation number is CGMCC NO.21077. The present invention adopts the Paecilomyces variotii fermentation liquid fermented liquid that endophytic fungus isolates to obtain as one of the raw materials of liquid culture medium, not only has increased Agaricum multiliposa ( Pholiota adiposa ) YX1 mycelium biomass, and reduce the cost of raw materials, to provide technical support for large-scale production. At the same time, the fermentation liquid of endophytic fungi is also fully utilized, which can promote the growth of other plants, without introducing other reagents, which is beneficial to the protection of the ecological environment.

Description

technical field [0001] The invention relates to the technical field of edible fungi, in particular to a mushroom strain YX1, a culture method and an application thereof. Background technique [0002] Edible fungi refer to a class of higher fungi with large fruiting bodies that can be eaten by humans. They are called mushrooms, mushrooms, and mushrooms by humans, such as Ganoderma lucidum, Hericium erinaceus, Cordyceps sinensis, shiitake mushrooms, and fungus that people are familiar with. Scientists have found that edible mushrooms are rich in nutrients, containing high protein, low fat, various amino acids, vitamins, trace elements, nucleotides, polysaccharides and other ingredients. The nutrients contained are very complete, and they are widely used in food and health care. [0003] In recent years, with the continuous improvement of people's living standards and self-care awareness, the cognition of the nutritional value of edible fungi has also been continuously improved...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G18/00A01G18/20A01G18/40A23L31/00A23L33/00A23L27/10A23K10/37A23K20/26C05F5/00C05F17/20C12R1/645
CPCC12N1/14A01G18/00A01G18/20A01G18/40A23L31/00A23L33/00A23L27/10A23K10/37A23K20/26C05F5/00C05F17/20A23V2002/00A23V2200/30Y02W30/40Y02P60/87Y02A40/20
Inventor 王洪凤丁军张晓英王珊珊孔波王庆彬
Owner SHANDONG PENGBO BIOTECHNOLOGY CO LTD
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