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A kind of Paecilomyces variotii strain and application thereof

A technology of Paecilomyces variotii and bacterial strains, applied to Paecilomyces variotii strains and its application fields, can solve problems such as unsatisfactory effects, and achieve the effects of avoiding incompatibility, accelerating the composting process, and promoting transformation

Active Publication Date: 2016-08-24
KWEICHOW MOUTAI COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This strain of Paecilomyces variotii has a good effect on degrading formaldehyde, but its effect on degrading cellulose and applying it to wine lees composting is not ideal

Method used

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  • A kind of Paecilomyces variotii strain and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Paecilomyces varioti strain MM2 was added to the 2 HPO 4 2g, (NH 4 ) 2 SO 4 1.4g, MgSO 4 .7H 2 O 0.3g, CaCl 2 0.3g, CoCl 2 2mg, FeSO 4 .7H 2 O 5mg, MnSO 4 .H 2 O 1.6mg, ZnSO 4 1.7mg, agar 20g, distilled water 1000mL, rice straw powder and bran 10.56g, wherein the rice straw powder and bran by sterilizing treatment are according to the ratio of 2:1, adjust pH to be 7.2 preparation rice straw bran liquid culture medium, in The liquid culture was carried out at 28° C. for 2 days to obtain Paecilomyces varioti strain MM2.

Embodiment 2

[0031] Paecilomyces varioti strain MM2 was added to the 2 HPO 4 2g, (NH 4 ) 2 SO 4 1.4g, MgSO 4 .7H 2 O 0.3g, CaCl 2 0.3g, CoCl 2 2mg, FeSO 4 .7H 2 O 5mg, MnSO 4 .H 2 O 1.6mg, ZnSO 4 1.7mg, agar 20g, distilled water 1000mL, rice straw powder and bran 3%, wherein the rice straw powder and bran by sterilizing process are according to the ratio of 1:1, adjust pH to be in the rice straw bran liquid culture medium of 7.3 preparation, in Liquid culture was carried out at 30° C. for 9 days to obtain Paecilomyces varioti strain MM2.

Embodiment 3

[0033] Paecilomyces varioti (Paecilomyces varioti) strain MM2 was added to the 2 HPO 4 2g, (NH 4 ) 2 SO 4 1.4g, MgSO 4 .7H 2 O 0.3g, CaCl 2 0.3g, CoCl 2 2mg, FeSO 4 .7H 2 O 5mg, MnSO 4 .H 2 O 1.6mg, ZnSO 4 1.7mg, 20g of agar, 1000mL of distilled water, 3% of rice straw powder and bran, wherein the sterilized rice straw powder and bran are adjusted to pH 7.3 according to the ratio of 1:1 for slant culture of rice straw bran liquid medium In the vessel, liquid culture was carried out at 28°C for 2 days to obtain a single-strain inoculum of Paecilomyces varioti strain MM2.

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Abstract

The invention relates to the technical field of biological strains, particularly a Paecilomyces variotii strain and application thereof. The Paecilomyces variotii strain MM2 is collected to the China General Microbiological Culture Collection Center on July 8th, 2013, and the collection number is CGMCC No.7903. The application method comprises the following steps: adding the strain into any of straw-bran liquid culture medium, filter paper-inorganic salt liquid culture medium or PDA (potato dextrose agar) liquid culture medium, and culturing at 28-30 DEG C for 2-9 days to obtain the single-strain solid microbial inoculant. The Paecilomyces variotii strain can efficiently degrade and utilize cellulose, can be used as an external strain source for recycling lignocellulose solid waste, and can accelerate the degradation of the cellulose; the Paecilomyces variotii strain is separated from wine sediment compost and applied to wine sediment compost, thereby avoiding the problem of difficulty in assessment; and after being added, the microbial inoculant containing the strain effectively promotes the rotting of the wine sediment compost and accelerates the processing of the organic fertilizer.

Description

technical field [0001] The invention relates to the technical field of biological strains, in particular to a strain of Paecilomyces variotii and its application. Background technique [0002] Distiller's grains, also known as distiller's grains, refer to the residue after alcohol or alcoholic beverages are evaporated from grains, that is, the dregs produced after brewing, and are often used as livestock feed. The annual output of distiller's dregs in some liquor production enterprises reaches tens of thousands of tons. If it is not disposed of in time, it will deteriorate and seriously pollute the surrounding environment. The use of vinasse compost to produce organic fertilizer can not only turn waste into wealth, but also help promote the development of rural economy and reduce environmental pollution. Since brewing companies use organic sorghum and organic wheat as the main raw materials for liquor production, the lees contain protein, cellulose, fat, vitamins and variou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F5/00C05F17/00C12R1/79
CPCY02A40/20Y02W30/40
Inventor 王和玉汪地强江友峰胡旭袁颉王岩
Owner KWEICHOW MOUTAI COMPANY
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