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A strain of Paecilomyces variotii with strong pathogenicity to citrus psyllids and its application

A technology of Paecilomyces variotii and citrus psyllids, applied in the field of biological control, to achieve the effects of no pollution to the environment, good market application prospects, and fast growth

Active Publication Date: 2018-08-31
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Beauveria bassiana (Balsamo) Vuillemin, Isaria fumosorosea (Wize) Brown et Smith, Hirsutella citriformis Speare, Lecanicillium lecanii (Zimmermann) have been used abroad. Viégas and Metarhizium anisopliae (Metchnikoff) Sorokin reported on the control of citrus psyllids, but so far in China, only Zhang Yanxuan et al. have carried out experimental research on the use of Amblyseius cucurbita carrying Beauveria bassiana to control citrus psyllids

Method used

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  • A strain of Paecilomyces variotii with strong pathogenicity to citrus psyllids and its application
  • A strain of Paecilomyces variotii with strong pathogenicity to citrus psyllids and its application
  • A strain of Paecilomyces variotii with strong pathogenicity to citrus psyllids and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation and screening of embodiment 1 citrus psyllid pathogenic bacteria

[0027] (1) Isolation and purification of pathogenic bacteria

[0028] The diseased citrus psyllids were collected from Gourita japonica in Tianhe District, Guangzhou City, Guangdong Province, and operated in a laboratory ultra-clean workbench. The citrus psyllid was first soaked in 70% ethanol for 30s, then soaked in 0.1% mercury liter for 1min, and finally rinsed with sterile water for 3 times. Use sterile filter paper to dry the water on the worm body, and then put it on the potato dextrose agar (PDA) medium (the preparation method is: weigh 200g potatoes, wash, peel and chop them, add 1000mL of water and boil for 20-30min, two Filter with a layer of gauze, add 20g of glucose and 15g of agar, fully dissolve and replenish water to 1000mL, then pack into Erlenmeyer flasks, sterilize under high pressure at 121°C for 20min).

[0029] Place the petri dish in a microbial incubator for constant te...

Embodiment 2

[0034] Identification of embodiment 2 bacterial strain GZMS-11

[0035] 1. Morphological characteristics of strain GZMS-11: strain GZMS-11 grows well on potato dextrose agar (PDA) medium, cultured at a constant temperature at 27°C, observes the growth of colonies every day and records the color and shape of colonies. Inoculate the strain GZMS-11 into the center of another PDA medium plate, insert the sterilized coverslip (1cm×1cm) obliquely into the medium about 1cm away from the inoculation point, and culture at a constant temperature of 27°C for 5 days After the hyphae climbed onto the cover glass, the cover glass was taken out and placed under an optical microscope to observe the hyphae and spore morphology of strain GZMS-11.

[0036] figure 1 It is the colony characteristic of strain GZMS-11 cultured on PDA medium for 10 days. It can be seen that the colonies of the strain GZMS-11 are round and fluffy, and the colonies are white at first, and later turn yellowish brown w...

Embodiment 3

[0042] The pathogenicity determination of embodiment 3 bacterial strain GZMS-11 to citrus psyllid

[0043] (1) The lethal medium concentration and lethal medium time of strain GZMS-11 to citrus psyllids

[0044] After the strain GZMS-11 was inoculated on the PDA solid medium for 10 days, the cultured colonies were rinsed with sterile water, and the conidia suspension was collected by filtration with sterile gauze, and serially diluted with sterile water to obtain concentrations of 1.0×10 8 spores / mL, 1.0×10 7 spores / mL, 1.0×10 6 spores / mL, 1.0×10 5 spores / mL, 1.0×10 4 spores / mL conidia suspension, and an appropriate amount of Tween-80 was added thereto to a final concentration of 0.1% (v / v). Put the healthy citrus psyllid in the conidia suspension containing 0.1% (v / v) Tween-80 of the above-mentioned concentrations as described in Example 1 for infiltration treatment, and then put it in a light incubator at 27±0.5°C (L:D=14:10, RH>90%) culture, and the citrus psyllid trea...

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Abstract

The invention discloses a Paecilomyces variotii strain with strong pathogenicity to citrus psyllids and an application thereof, belonging to the technical field of biological control. The strain is Paecilomyces variotii GZMS‑11, which was deposited in China Center for Type Culture Collection on October 29, 2015, with the preservation number CCTCC NO: M 2015651. Paecilomyces variotii GZMS-11 described in the present invention has strong pathogenicity to citrus psyllids, and can be developed as a biological pesticide for the biological control of citrus psyllids, compared with the current chemical agents for preventing and treating citrus psyllids , has the advantages of high efficiency, green, environmental protection, long-lasting effect and not easy to produce drug resistance, and has no pollution to the environment and no residue. The strain GZMS-11 has fast growth speed, large spore production, high spore germination rate, is easy to prepare, and has good market application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological control, and particularly relates to a Paecilomyces variotii strain with strong pathogenicity to citrus psyllids and an application thereof. Background technique [0002] Citrus Huanglongbing is the most devastating disease in the citrus industry. It can infect a variety of citrus plants. There is no effective therapeutic agent and no disease-resistant (tolerant) varieties available. Citrus Huanglongbing is currently spreading across the world. The gradual spread of growing areas is a major threat to the world citrus industry. The citrus psyllid (Diaphorina citri kuwayamae) belongs to the family Psyllidae of the order Homoptera. It mainly harms Rutaceae plants such as murella and citrus. Adults and nymphs gather in new shoots to suck juice. Deformation is easy to fall off, seriously affecting plant growth, and is one of the important pests on citrus. The citrus psyllid is the only insect vect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/79
Inventor 宋晓兵彭埃天程保平凌金锋周娟陈霞
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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