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Penicillium composition with low-temperature protection effect, low-temperature protective agent, application of low-temperature protective agent and method for improving survival rate of floating seedlings

A cryoprotectant and cryoprotection technology, which are applied in the fields of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of food safety, environmental pollution, complicated operation, etc., and achieve low cost and safety. No pollution, simple operation effect

Active Publication Date: 2022-03-08
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, methods such as environmental heating, covering and shading, and adding chemical agents are mainly used to prevent low-temperature damage during seedling cultivation, but these are often accompanied by problems such as high cost, cumbersome operation, environmental pollution, and food safety.

Method used

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  • Penicillium composition with low-temperature protection effect, low-temperature protective agent, application of low-temperature protective agent and method for improving survival rate of floating seedlings

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preparation example Construction

[0028] In the present invention, the preparation method of the metabolite preferably includes the following steps:

[0029] respectively activating and culturing Penicillium citrinum, Penicillium rubrum, Penicillium Thomme and Paecilomyces variotii to obtain the bacterium liquid of each Penicillium;

[0030] The low-temperature protective agent is obtained by sequentially performing high-temperature inactivation, drying and pulverization on the bacterial agent obtained above.

[0031] In the present invention, Penicillium citrinum, Penicillium rubrum, Penicillium thomerii and Paecilomyces variotii are respectively activated and then cultivated to obtain bacterial liquid of each Penicillium.

[0032] In the present invention, when activating Penicillium citrinum, it preferably includes sterile water activation and culture medium activation. In the present invention, the process of said sterile water activation is preferably to scrape the bacterium colony of Penicillium citrinu...

Embodiment 1

[0049] (1) Prepare medium:

[0050] Cha's medium (Penicillium citrinum): 30.0 g of sucrose, NaNO 3 3.0g, MgSO 4 ·7H 2 O 0.5g, KCl 0.5g, FeSO 4 4H 2 O 0.01g, K 2 HPO 4 1.0g, dilute to 1.0L with distilled water, pH 7.0-7.2;

[0051] PDA medium (Penicillium rubrum): 200g of potatoes, 20g of glucose, dilute to 1000mL with deionized water;

[0052] Malt extract medium (Penicillium Thomme): malt extract 20.0g, dilute to 1.0L with tap water, natural pH;

[0053] 5°Bé wort culture medium (Paecilomyces variotii): 1.0L of 5°Bé wort, natural pH.

[0054] Put the medium prepared above into an autoclave to sterilize at 121°C for 20 minutes;

[0055] (2) Activation and cultivation: Scrape the colony of Penicillium rubrum (just pick a small colony with a bamboo stick) and inoculate it in a 250mL triangular flask filled with 100mL sterile water, at a temperature of 25°C and a rotation speed of 120r Under the condition of 30 min / min, vibrate for 30 min to fully disperse the spore mas...

Embodiment 2

[0062] The application method of the low-temperature protective agent for tobacco seedling cultivation in tobacco seedling cultivation:

[0063] (1) The cryoprotectant I prepared in Example 1 is added to the nutrient solution of floating seedlings at a concentration of 15.00g / kg, and the fungicide (80% carbendazim wettable powder, Sichuan Jiade Li Technology Development Co., Ltd.) is added to the nutrient solution of floating seedlings at a concentration of 100 mg / L to obtain a mixed solution; the nutrient solution is an ammoniated potassium sulfate compound fertilizer solution, and the ammonified potassium sulfate compound fertilizer solution is used to compound potassium sulfate. The concentration of fertilizer is 20g / L (Anhui Zhongyuan Chemical Fertilizer Co., Ltd.);

[0064] (2) Tobacco seeds are placed in the floating polystyrene seedling tray, a total of 84 seeds are placed in the water tank containing the above-mentioned mixed solution subsequently;

[0065] (3) Cultur...

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Abstract

The invention belongs to the technical field of agricultural production and biology, and particularly relates to a penicillium composition with a low-temperature protective effect, a low-temperature protective agent, application of the penicillium composition and the low-temperature protective agent and a method for improving the survival rate of floating seedlings. The invention provides a penicillium composition with a low-temperature protection effect. The penicillium composition comprises penicillium citrinum, penicillium rubrum, penicillium torusii and paecilomyces variotii. The penicillium composition can effectively improve the survival rate and the cold resistance of floating breeding, has the advantages of being low in cost and easy to operate, and has the advantages of being high in safety and free of pollution due to the fact that microorganisms are adopted.

Description

technical field [0001] The invention belongs to the fields of agricultural production and biotechnology, and in particular relates to a penicillium composition with low-temperature protection effect, a low-temperature protection agent and its application, and a method for improving the survival rate of floating seedlings. Background technique [0002] Tobacco is an important economic crop in my country, and seedling cultivation is an important link in tobacco production. At present, my country adopts the floating seedling cultivation method to carry out tobacco seedling cultivation. For example, the "Technical Regulations for Tobacco Intensive Seedling Cultivation" (GB / T25241.1-2010) released the methods and standards for seedling cultivation. However, seedling cultivation needs to be carried out in winter, and tobacco is a tropical crop that likes temperature and has poor tolerance to low temperature. Tobacco seedlings in cold regions are susceptible to freezing damage and ...

Claims

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Application Information

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IPC IPC(8): A01N63/36A01N63/30A01P21/00C12N1/14A01G31/00C12R1/80C12R1/79
CPCA01N63/36A01N63/30C12N1/14A01G31/00Y02P60/21
Inventor 陈穗云牟宗敏赵大克李倩倩
Owner YUNNAN UNIV
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