175 results about "Eupenicillium molle" patented technology

The present invention relates to a method of enhancing growth conditions for plants by growing the plants in soil containing, in proximity to the plant roots, both a phosphorus source and at least two strains of the fungus Penicillium, particularly P. bilaiae, more particularly strains NRRL 50162 and NRRL 50169.

The invention provides a penicilliums p. strain and application thereof. Eucalyptus endophyte is a penicilliums p. strain 1, and the collection number is CGMCC No.4297. The eucalyptus endophyte inoculated to eucalyptuses can improve the low phosphorus stress resistance of the eucalyptuses and promote phosphorus absorption of the eucalyptuses. Because the eucalyptus endophyte is used for promoting the phosphorus absorption of the eucalyptuses, and when the strains are inoculated to the eucalyptus plants, the activities of the plant phosphatase and the soil phosphatase can be improved, the pH value of the soil rhizosphere can be reduced, and the plant P utilization rate is greatly improved; therefore, the purpose of promoting normal growth of the plants in the low phosphorus soil is fulfilled, and the utilization rate of the soil phosphate fertilizer is greatly reduced.

The invention discloses a penicilliumgriseofulvum XJ-EP-058 strain as well as a microbial preparation resisting banana vascular wilt and application thereof, belonging to the technical field of microbial pesticides. The strain is penicilliumgriseofulvum XJ-EP-058 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.5467. The microbial preparation, containing the strain, for resisting the banana vascular wilt is obtained by test tube seed culture, liquid seed culture for the strain and liquid fermentation culture for the strain. The strain and the microbial preparation, containing the strain, for resisting the banana vascular wilt have obvious in-vitro and in-vivo preventing effects and have the germination inhibition rate reaching 100% to spores of banana vascular wilt bacteria, and the average diameter of bacteriostatic zones to the banana vascular wilt bacteria is 14.5mm; and a result of a greenhouse potted living organism test shows that the strain and the microbial preparation have the average control effect reaching 74.38% on the banana vascular wilt bacteria. The microbial preparation has the advantages of low raw material cost and simple and feasible production process.

A ferment extract from a bacterial strain the Eupenicillium crustaceum species useful in the cosmetic treatment and / or care of the skin, mucous membranes, hair and / or nails and cosmetic uses of same.

The invention discloses a penicillium vulpinum fungi strain and a method for preparing levo 7-hydroxyl butylphthalide ((S)-(-)-butyl-7-hydroxyl phthalide) employing the fungi strain. The fungi strain disclosed by the invention is a penicillium vulpinum fungi strain NCC3421 (penicillium vulpinum) and is preserved at the China General Microbiological Culture Collection Center (CGMCC for short); and the preservation number is CGMCC No.9094. Under the culture condition provided by the method, the fermentation unit of the levo 7-hydroxyl butylphthalide reaches over 1600mg / L; the fermented product is relatively single in components, and easy to separate and purify, and can be applied to industrialized production of the levo 7-hydroxyl butylphthalide.

Disclosed are: a eukaryotic amadoriase which is prepared by introducing a mutation into DNA encoding a eukaryotic amadoriase derived from a microorganism belonging to the genus Coniochaeta or Eupenicillium so as to introduce a substitution into a specific amino acid residue in the eukaryotic amadoriase, thereby overcoming the defect associated with thermal stability; a gene or recombinant DNA for the eukaryotic amadoriase; and a process for production of a eukaryotic amadoriase having excellent thermal stability.

Methods and compositions comprising essential oils or components thereof for protecting harvested fruits against decay caused by a specific pathogenic fungus are provided, such as Cinnamon cassia oil for protection of persimmons against Alternaria alternata, strawberries against Botrytis cinerea, or mandarins against Penicillium italicum or Penicillium digitatum.

The invention discloses a high-activity composite cellulase and preparation thereof, and an application method for the same in enzymatic saccharification of wood fiber. According to the invention, Eupenicillium javanicum ZN-205 is subjected to shake-flask fermentation so as to produce beta-glucosidase; best enzyme production conditions are that an initial pH value is 6, the concentration of peptone is 0.75%, the concentration of microcrystalline cellulose is 2.5%, the addition amount of tween-80 is 0.05%, culture temperature is 28 DEG C, the volume of liquid filled in a 250 ml triangular flask is 100 ml, a revolving speed of a shaker is 175 r / min, inoculum amount is 5% and the highest activity of beta-glucosidase is 2.312 IU / ml; beta-glucosidase prepared from Eupenicillium javanicum ZN-205 and cellulase prepared from Trichoderma reesei Rut C-30 are compounded, and an obtained optimal ratio of beta-glucosidase activity to filter paper activity of 1.4. Utilization of the compounded enzyme for enzymatic saccharification of wood fiber enables high-efficiency and low-cost enzymatic saccharification effects to be achieved.

The invention relates to marine streptomyces with antibacterial activity and belongs to the technical field of microbes. The marine streptomyces is streptomyces chumphonensis AM-4, is registered and collected in China General Microbiological Culture Collection Center on October 30, 2017 and has a collection number of CGMCC No.14843. The streptomyces chumphonensis AM-4 has an excellent inhibitory effect on ccolletotrichum glecosporioides, penicillium italicum, penicillium digitatum and pathogen of brown stem-end rot, and has the potential of being developed into a biopesticide.

The invention relates to a treatment method for dephosphorizing and denitrifying sewage. The method is characterized by carrying out treatment by combining a physical method and a biological agent, wherein the biological agent comprises the following raw materials in parts by weight: 5-7 parts of paracoccus denitrificans, 5-7 parts of saccharomyces cerevisiae, 4-5 parts of rhodococcus, 4-5 parts of bacillus amyloliquefaciens, 3-4 parts of clostridium papyrosolvens, 3-4 parts of penicillium ochrochloron, 2-3 parts of pseudomonas and 1-2 parts of bacillus cereus. The biological agent contains various microorganisms with excellent capacity of degrading refractory pollutants, is reasonable in compatibility of the strains, has good degrading effects and has a broad application prospect.

The invention discloses a marine fungi penicillium crustosum bacterial strain, quinolinone compounds derived from marine fungi penicillium crustosum, and a preparation method and applications of the quinolinone compounds. Specific structural formulas of the quinolinone compounds are represented by formula R1-formula R6. According to the preparation method, a culture medium is inoculated with the marine fungi penicillium crustosum bacterial strain AP2T1 CGMCC No.8516 for static fermentation; an obtained fermentation broth is extracted with ethyl acetate, and mycelium is extracted with an organic solvent; obtained crude extracts are mixed, an obtained mixture is subjected to sephadex column chromatography, reverse-phase silica-gel column chromatography, and thin layer chromatography detection using organic solvents; an obtained elution ingredient containing intermediates is subjected to methylation, and thin layer chromatography and reverse-phase silica-gel column chromatography separation and purification so as to obtain target compounds R1-R6. The target compounds R1-R6 possesses wide application prospect in preparation of antitumor drugs, antibacterial agents, and agricultural insecticides.

The invention discloses tephrosia purpurea endophytic fungus penicillium griseofulvum TPL25 and application of a fermentation product of the tephrosia purpurea endophytic fungus penicillium griseofulvum TPL25 in plant disease prevention fungal pathogens. The tephrosia purpurea endophytic fungus penicillium griseofulvum TPL25 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2014607. Fungus fermentation liquor, fermentation liquor extractives and mycelium extractives have the strong mycelium grow inhibition function on sclerotinia rot of colza, tobacco blackleg and other phytopathogen, excellent strains are provided for development of microbial pesticide, and in addition, the tephrosia purpurea endophytic fungus is studied for the first time.

The invention relates to a penicillium xfsf-8 strain and application thereof to preparation of fungicide for resisting tobacco bacterial wilt. The bio-control penicillium xfsf-8 strain is obtained through the steps that a soil sample is taken from a healthy tobacco field in the Enshi Prefecture, and screening, analysis and culture are conducted. It is verified through a field experiment that the penicillium xfsf-8 strain has a good effect of preventing and treating tobacco bacterial wilt, is high in pertinence and free of pollution, and can be stored for a long time after being prepared into the fungicide, field application is convenient, results in the actinomycete bio-control bacillus research field are further enriched, and guiding significance is achieved for preventing and treating tobacco bacterial wilt.

The invention relates to Penicillium citrinum CR-2CGMCC 3024 and an application thereof. The bacterium is Penicillium fungus, which has short cultivating period and high propagation speed. An abundant of uniform cellulose degradation enzymes can be cultivated at short time for generating fungus culture solution. The invention also provides a method for generating endoglucanase and exoglucanase by fermenting the bacterial strain in the liquid enzyme culture medium, which can produce endoglucanase and exoglucanase with higher vigor at short time, with simple and stable fermenting technique, low cost and high yield.

The invention discloses penicillium sclerotiorum for poisoning plant parasitic nematodes and a preparation method and application of the penicillium sclerotiorum. The strain number of the penicillium sclerotiorum is D35, and the registering serial number of the penicillium sclerotiorum in the China General Microbiological Culture Collection Center is CGMCC No.12168.A plant parasitic nematode inhibitor which is extracted from cultures of the penicillium sclerotiorum D35 through ethyl acetate and dissolved in ethyl acetate serves as an active ingredient, the corrected death rate on meloidogyn incognita is 100%, and the average inhibition rate of the penicillium sclerotiorum D35 on meloidogyn incognita egg hatching is 99%. On the condition that no auxiliary is added into the solid fermentation cultures of the penicillium sclerotiorum D35, the prevention effect on a cucumber root-knot nematode disease at the 45 day reaches 83.9%, and the prevention effect on the cucumber root-knot nematode disease after 80 days still reaches 77.7%.Cucumber seedlings treated with the penicillium sclerotiorum D35 are developed in root system, few in root knot and normal in plant growth, and the penicillium sclerotiorum D35 is safe to cucumbers.

The invention discloses a strain Q2 which produces Penicillium purpureogenum and has been preserved in China General Microbiological Culture Collection Center (CGMCC for short) on 25th, October, 2016. The address is Institute of Microbiology, CAS, #3, Yard 1, West Beichen Road, Chaoyang District, Beijing. The preservation number of the strain is CGMCC NO.13165. According to the invention, the strain Q2 which produces Penicillium purpureogenum is separated from rhizosphere soil of cucumbers for the first time. The strain plays a relatively strong antagonistic role to a plant soil-borne disease bitter gourd wilt and provides a novel biocontrol bacterium for preventing and treating probable bitter gourd wilt and soil-borne diseases of other horticultural plants, so that the types of biocontrol agents are increased, and the phenomenon that the biocontrol agents are single in the market is overcome.

The invention discloses a penicillium oxalicum for reducing hexavalent chromium and a screening method thereof, and belongs to the technical field of microorganisms. The penicillium oxalicum is named as penicillium oxalicum SL2, and is stored in the China Center for Type Culture Collection on Oct. 22, 2014, and the preservation number of the penicillium oxalicum SL2 is CCTCC M 2014505. The bacterial strain can grow up in a PDA culture medium, in which the concentration of Cr (VI) is 2,000 mg / L, tolerates a NaCl saline solution of 100 g / L, and can completely reduce Cr (VI) of 357.9 mg / L in the culture medium within five days. The penicillium oxalicum for reducing the hexavalent chromium can be applied to treating Cr (VI) pollution in the environment.

The invention discloses a gene highly expressing a nuclease P1 and an expression vector and a host cell comprising the gene and an application for the gene. The invention discloses a recombinant pichia yeast CGMCC No.3188 expressing the gene of nuclease P1 produced from Penicillium citrinum and its construction method. The inventive recombinant engineering strain pichia yeast CGMCC No.3188 can highly express Penicillium citrinum nucP gene. The secretion-expressed nuclease P1 has high phosphodiesterase activity, is used for hydrolyzing RNA, has high product yield and little generated impurity and reduces working procedure of downstream separation as well as decreases production cost. The invention uses the recombinant engineering strain pichia yeast CGMCC No.3188 to perform high density fermentation. The enzyme activity is 1.5 times by the wild strains. The acquired recombinase is applied to RNA solution enzymatic hydrolysis, thus, reaching the hydrolysis rate of more than 40% and the acquired hydrolysate by-product is substantially reduced.

The invention relates to a biological preparation for treating sodium glutamate fermentation sewage. The biological preparation comprises a physical preparation and a complex microbial agent, wherein the complex microbial agent comprises the following components in parts by weight: 4-5 parts of Azotobacter chroococcum, 4-5 parts of phanerochaete chrysosporium, 5-6 parts of Bacillus amyloliquefaciens, 4-6 parts of Clostridium papyrosolvens, 3-4 parts of penicillium ochrochlorron, 3-4 parts of Pseudomonas aeruginosa and 2-3 parts of bacillus cereus. The biological preparation contains multiple microorganisms having excellent degradation capacity to non-biodegradable pollutants, and by virtue of reasonable compatibility of the strains, the good degradation effect is achieved, and the application prospect of the biological preparation is wide.

The invention discloses a preparation method of penicillium oxalicum and application of penicillium oxalicum in phosphate solubilizing, growth promoting and fusarium graminearum antagonism, and belongs to the technical field of environmental biology. The penicillium oxalicum MEM02 can inhibit germination of fusarium graminearum spores in soil and remarkably reduce infection of the fusarium graminearum spores to wheat, and meanwhile the penicillium oxalicum MEM02 can efficiently solubilize phosphorus and promote plant growth. Therefore, the penicillium oxalicum MEM02 has good application prospects in the aspect of developing new biopesticides or new biological control microbial agents.

A combination product including a mixture of 3-iodpropynyl-N-n-butylcarbamate (“IPBC”) and 2-n-N-butyl-1,2-benzisothiazolin-3-one (“BBIT) for use as a fungicide and growth inhibitor in industrial applications. Combination products of the present invention are effective for inhibiting the growth of microorganisms, and exhibit a synergistic antifungal effect against fungi such as, for example, Aspergillus niger, Penicillium funiculosum, Trichoderma virens, Chaetosphaeridium globosum, Penicillium sp., and Caratocystis Pilifera. Methods for using the product in metal working fluids, dry film coatings, in-can preservatives, caulks, sealants leather, wood, rope, cordage, textiles, ink, adhesives and polymeric materials are also described.

The invention discloses a Polygonum cuspidatum root endophytic strain. The strain is named Penicillium oxalicum G3 in a classified way, the strain was collected in China General Microbiological Culture Collection Center on January 17, 2011, and the collection number is CGMCC NO.4558. Polydatin in Polygonum cuspidatum can be transformed into resveratrol by inoculating the strain into a culture medium containing Polygonum cuspidatum boiling extracting solution, so that the polydatin can be directly transformed into the resveratrol through microbial fermentation, the extraction of the polydatin is not required, the cost can be saved, and the pollution to the environment can be reduced.

A methylotrophic bacillus strain (Bacillus methylotrophicus) for biocontrol and application thereof are disclosed. The bacterium Bacillus methylotrophicus BCN2 is deposited in the China General Microbiological Culture Collection Center under the accession number CGMCC No: 16443. The invention comprises isolation and culture of the strain and molecular identification of 16S rDNA; effective inhibiting effects of Methylotrophic Bacillus volatiles against common spoilage fungi (Fusarium oxysporum, Staphylococcus aureus, Trichoderma viride, Colletotrichum gloeosporioides, Penicillium expansum) ontwo-zone plate and loquat fruits and the antiseptic application of the volatile matter in loquat storage. The volatile antimicrobial substances produced by Bacillus methylotrophicus (BCN2) could not contact fruit directly, It can be used for preparing biocontrol fresh-keeping agent which can inhibit spoilage fungi such as Fusarium oxysporum, Staphylococcus aureus, Trichoderma viride, Colletotrichum gloeosporioides, Penicillium expansum and so on, and has wide bacteriostasis spectrum.

The invention discloses a method for improving the salt resistance of turf by adopting a reinforced garbage compost microbial agent. The reinforced salt resistant microbial agent comprises the following components: reinforced bacillus subtilis, reinforced lysine bacillus and reinforced penicillium chrysogenum, with the weight ratio of 1: 1: 1. The method disclosed by the invention adopts the following steps: gradually increasing the concentration of NaCl to reinforce microorganisms in urban garbage compost, and finally performing the screening so as to obtain the prepared reinforced salt resistant microbial agent. The obtained reinforced salt resistant microorganisms of the reinforced bacillus subtilis, the reinforced lysine bacillus and the reinforced penicillium chrysogenum are compounded into composite microbial agents in different concentrations, the composite microbial agents are inoculated to a planting system of the turf, so that the optimal concentration of the composite reinforced salt resistant microbial agent is screened out, the salt resistance of the turf is improved, and the technical support is provided for planting the carpet turf on the substrate of saline-alkali soil.

The invention belongs to the microorganism field. A solid culture medium taking Ca3(PO4)2 as a unique phosphorus source is utilized for screening a strain 14 with a phosphorus-dissolving effect, and the phosphorus-dissolving capacity of a to-be-tested phosphorus-dissolving strain is determined by virtue of an inorganic phosphorus liquid culture medium shake flask experiment. The inorganic phosphorus liquid culture medium shake flask experiment determines that the phosphorus-dissolving capacities of penicillium strains QM-6 and QM-10 are relatively high, and the maximum soluble phosphorus contents of the two corresponding strains are respectively 1239.21mg / L and 1386.93mg / L. The strains QM-6 and QM-10 are preliminarily determined as Penicillium oxalicum by virtue of an ITS sequence determination method. The determination of a greenhouse pot culture phosphorus-dissolving experiment and the analysis of a growth-promoting effect find that the phosphorus-dissolving strains QM-6 and QM-10 are capable of converting unavailable phosphorus which is difficult to be absorbed and utilized by plants in soil into soluble available phosphorus which can be directly absorbed and utilized and well promoting the growth of the plants and have important theoretical and practical significances to reduction of the application amount of phosphorus fertilizers.

The invention relates to a biochemical preparation for treating glutamic acid fermentation sewage. The biochemical preparation contains a physical adsorbent and a composite microbial preparation, wherein the composite microbial preparation is prepared from the following raw materials in parts by weight: 6-8 parts of azotobacter chroococcum, 4-6 parts of Rhodococcus sp., 4-6 parts of bacillus amyloliquefaciens, 3-5 parts of clostridium papyrosolvens, 3-5 parts of penicillium ochrochlorron, 3-4 parts of Pseudomonas stutzeri and 2-3 parts of bacillus cereus. The biochemical preparation contains a variety of microbes which have excellent degradation capability to refractory contaminants, and all strains are reasonably combined, so that the biochemical preparation has a good degradation effect and has a broad application prospect.