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Antagonistic fungus for preventing and treating bitter gourd wilt and application thereof

A technology of balsam pear and strains, applied in the fields of application, fungi, pest control, etc., can solve the problems of pesticide residues, difficulty in adapting to the requirements of planting models, and large environmental pollution, so as to reduce the use of chemical fertilizers and pesticides, which is conducive to pollution-free The effect of production and increasing economic income

Active Publication Date: 2017-03-22
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, no effective disease-resistant varieties for this disease have been bred. In the past, chemical agents such as methyl bromide can be used to effectively control the occurrence of Fusarium wilt, but it is very polluting to the environment, and it is easy to cause drug resistance of the bacteria, and it will also lead to problems such as pesticide residues. , causing serious damage to the ecosystem, threatening human health and food safety, has been banned by most countries in the world
Although measures such as crop rotation and soil disinfection have a certain effect on the control of Fusarium wilt, their production costs are relatively high, and it is difficult to adapt to the requirements of China's intensive agricultural planting model

Method used

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  • Antagonistic fungus for preventing and treating bitter gourd wilt and application thereof
  • Antagonistic fungus for preventing and treating bitter gourd wilt and application thereof
  • Antagonistic fungus for preventing and treating bitter gourd wilt and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Isolation and identification of bacterial strains

[0032] 1. Isolation and screening of strains:

[0033] (1) Separation of strains

[0034] The cucumber rhizosphere soil collected from the experimental base of Shandong Agricultural University in Tai'an City, Shandong Province was separated by the soil dilution coating plate method. 3 、10 4 、10 5 、10 6 Evenly spread the diluted solution on the PDA medium plate prepared in advance, culture at a constant temperature of 25°C, observe day by day after 3 days, and pick the mycelium block in time to transfer to a new PDA plate when there is mycelium growing Continue to cultivate. When the spores are to be produced, single spores are isolated and purified, the purified strains are numbered, and the slant is preserved for future use.

[0035] PDA medium formula: potato 200g, glucose 20g, agar 17g, tap water 1000ml.

[0036] (2) Screening of antagonistic bacteria

[0037] The strains isolated and purified fro...

Embodiment 2

[0060] Embodiment 2: the preparation of bacterial strain Q2 biocontrol agent

[0061] The specific preparation method is as follows:

[0062] 1. Preparation of Seed Solution

[0063] The bacterial classification of the Penicillium purpureogenum (Penicillium purpureogenum) Q2 bacterial strain (CGMCC No.13165) isolated and preserved in Example 1, which was preserved on the slant of the test tube at 4°C, was transferred to a fresh PDA medium plate for activation, and cultivated at 25°C After 5 days of growing fresh mycelia, they were transferred to a large number of fresh PDA plates and incubated at 25°C for 10 days. After the spores are produced, scrape them off under aseptic conditions, and prepare them with sterile water to a concentration of 1×10 8 The spore suspension in cfu / ml is the seed solution of strain Q2.

[0064] PDA medium formula: potato 200g, glucose 20g, agar 20g, water 1000ml.

[0065] 2. Preparation of biocontrol agents and antibacterial active substances ...

Embodiment 3

[0067] Embodiment 3: the application of producing Penicillium purpura strain Q2 in the control of bitter gourd wilt

[0068] 1. Test method:

[0069] The experiment consisted of two treatments, namely:

[0070] (1) Treatment group (Q2): In a shaker at 33°C and 200r / min, soak the seeds of bitter gourd in the spore suspension for 17 hours, wipe off the water on the surface of the seeds, and place them in sterilized gauze at 33°C. Germinate at constant temperature for 48-64h, and keep moist with the spore suspension of strain Q2.

[0071] (2) Control group (CK): The treatment methods and conditions of bitter gourd seeds were the same as those of the treatment group, and the spore suspension was replaced with sterile water for soaking and moisturizing.

[0072] After accelerated germination, the Lubai seeds were sown in a seedling tray filled with sterile soil, and the seedlings were raised in a light incubator (16 hours of light, 28°C, 8 hours of darkness, 22°C), under conventi...

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Abstract

The invention discloses a strain Q2 which produces Penicillium purpureogenum and has been preserved in China General Microbiological Culture Collection Center (CGMCC for short) on 25th, October, 2016. The address is Institute of Microbiology, CAS, #3, Yard 1, West Beichen Road, Chaoyang District, Beijing. The preservation number of the strain is CGMCC NO.13165. According to the invention, the strain Q2 which produces Penicillium purpureogenum is separated from rhizosphere soil of cucumbers for the first time. The strain plays a relatively strong antagonistic role to a plant soil-borne disease bitter gourd wilt and provides a novel biocontrol bacterium for preventing and treating probable bitter gourd wilt and soil-borne diseases of other horticultural plants, so that the types of biocontrol agents are increased, and the phenomenon that the biocontrol agents are single in the market is overcome.

Description

technical field [0001] The invention relates to an antagonistic fungus for preventing and treating balsam pear wilt and its application, in particular to a penicillium violet-producing fungus with remarkable control effect on balsam pear wilt and its application, which belongs to the field of biotechnology. Background technique [0002] Bitter gourd wilt is a soil-borne disease caused by Fusarium oxysporumf.sp.momordicae infecting bitter gourd. It is widely distributed in various bitter gourd producing areas in the world. All can take place, can make a large amount of dead vines appear in the melon field when the disease is prevalent, reduce production more than 30%, have a strong impact on the yield and quality of bitter gourd. [0003] At present, no effective disease-resistant varieties for this disease have been bred. In the past, chemical agents such as methyl bromide can be used to effectively control the occurrence of Fusarium wilt, but it is very polluting to the env...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12R1/80
CPCA01N63/30C12N1/145C12R2001/80Y02A40/10
Inventor 高克祥田叶韩刘晓光王永阳何邦令
Owner SHANDONG AGRICULTURAL UNIVERSITY
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