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Penicillium oxalicum for reducing hexavalent chromium and screening method thereof

A Penicillium oxalate, screening method technology, applied in the field of microorganisms, can solve the problems of limited application, difficult to tolerate high salt conditions, poor adaptability and the like

Active Publication Date: 2015-04-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the isolated Cr(VI)-reducing bacteria have poor adaptability and cannot tolerate high-salt conditions under actual pollution conditions, which limits their application in actual Cr(VI) pollution remediation

Method used

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  • Penicillium oxalicum for reducing hexavalent chromium and screening method thereof
  • Penicillium oxalicum for reducing hexavalent chromium and screening method thereof
  • Penicillium oxalicum for reducing hexavalent chromium and screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the screening method of the Penicillium oxalicum of a strain reduction Cr (VI)

[0020] 1.1 Make a PDA plate containing Cr(VI): Weigh 20g of potatoes and add them to 100ml of water, boil for 30min, filter through double-layer gauze, replenish water to 100ml, add 1g of glucose, 1.5g of agar, sterilize at 115°C for 20min, cool until it is not hot, Add potassium dichromate solution sterilized by a 0.22 μm sterile filter head to make the culture medium contain 300 mg / L of Cr(VI), and pour the plate in a sterile operating table.

[0021] 1.2 Make Cr(VI)-containing liquid medium: Weigh 20g of potatoes and add to 100ml of water, boil for 30min, filter through double-layer gauze, replenish water to 100ml, add 1g of glucose, sterilize at 115°C for 20min, cool until it is not hot, add 0.22 Potassium dichromate solution sterilized with a μm sterile filter head, so that the medium contains 300 mg / L of Cr(VI).

[0022] 1.3 Collection: Place the Cr(VI)-containing PDA p...

Embodiment 2

[0026] Example 2: Morphology, Carbon Source Utilization, and Molecular Biological Identification of Bacterial Strain SL2

[0027] The detection and identification of strain SL2 was entrusted to the China Center for Type Culture Collection, and the results are as follows:

[0028] 2.1 Morphological features

[0029] Colonies of strain SL2 were developed and velvety. dark green( figure 1 ). Conidia chain integrated cylindrical, conidia oval P ( figure 2 ).

[0030] 2.2 Characteristics of carbon source utilization

[0031] The utilization of 95 carbon sources by SL2 was detected by biolog FF plate, and the results showed that it could utilize 56 of them.

[0032] Table 1 Carbon source utilization of SL2 strain

[0033] Test items result Test items result A1 water B7 D-galactose ＋ A2 Tween 80 ＋ B8 D-galacturonic acid ＋ A3 N-acetyl-galactosamine － B9 Gentiobiose ＋ A4 N-acetyl-glucosamine ＋ B10 D-gluconic ...

Embodiment 3

[0041] Embodiment 3: Salt tolerance, Cr (VI) tolerance detection of bacterial strain SL2

[0042] Make the liquid PDA culture medium that contains NaCl concentration and be 0g / L, 20g / L, 100g / L. Press 10 4 cells / mL inoculated with SL2

[0043] For the spores, the culture system was placed in a shaker at 30°C and 200 rpm for shaking culture. The mycelia were filtered daily and weighed ( image 3 ). The results showed that SL2 had strong salt tolerance and could grow in PDA medium with NaCl concentration exceeding 100g / L.

[0044] Add potassium dichromate solution sterilized by filtration through a 0.22 μm filter membrane into the liquid PDA medium to prepare a PDA medium containing Cr(VI) at a concentration of 2000 mg / L. Press 10 4 The spores / mL were inoculated with SL2, and the culture system was shaken in a shaker at 30°C and 200 rpm. The mycelia were filtered daily and weighed ( Figure 4 ). The results showed that SL2 had strong tolerance to Cr(VI), and could grow ...

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Abstract

The invention discloses a penicillium oxalicum for reducing hexavalent chromium and a screening method thereof, and belongs to the technical field of microorganisms. The penicillium oxalicum is named as penicillium oxalicum SL2, and is stored in the China Center for Type Culture Collection on Oct. 22, 2014, and the preservation number of the penicillium oxalicum SL2 is CCTCC M 2014505. The bacterial strain can grow up in a PDA culture medium, in which the concentration of Cr (VI) is 2,000 mg / L, tolerates a NaCl saline solution of 100 g / L, and can completely reduce Cr (VI) of 357.9 mg / L in the culture medium within five days. The penicillium oxalicum for reducing the hexavalent chromium can be applied to treating Cr (VI) pollution in the environment.

Description

technical field [0001] The invention relates to a Penicillium oxalicum SL2 capable of reducing Cr(VI) and a screening method thereof, belonging to the technical field of microorganisms. Background technique [0002] Chromium pollution is a serious environmental problem facing our country at present. my country is the largest producer of chromium slag in the world. Untreated chromium slag has been washed and infiltrated by rainwater for many years, seriously polluting the storage site environment; sewage and waste from chromium-related industries such as steel, electroplating, and leather have not been properly treated. Affect the water and soil environment in related areas. Serious chromium pollution has triggered vicious environmental events such as chromium pollution in Qujing, Yunnan, which has aroused great concern from the government and all walks of life. Therefore, the remediation and treatment of chromium pollution is urgent. [0003] Chromium exists mainly in two ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/80
CPCC12N1/02C12N1/145C12R2001/80
Inventor 施积炎龙碧波何俊昱叶基恩叶斌晖张舒徐辰邹丽娜
Owner ZHEJIANG UNIV
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