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Production technology for extracting berberine from cortex phellodendri and coptidis rhizoma through complex enzyme method

A production process and compound enzyme technology, which is applied in the fields of traditional Chinese medicine pharmaceuticals, veterinary drugs, feed or raw material processing, and pesticides, can solve problems such as easy pollution, and achieve the effects of low cost, simple operation, and simple process

Active Publication Date: 2016-07-13
天津尚美化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the dilute sulfuric acid method mainly utilizes the sulfate in berberine to be dissolved in an acidic solution, and first converts berberine into sulfate and dissolves it in acid, then adds hydrochloric acid and then converts it into hydrochloride for precipitation. The advantage is that the material is cheap and the process Simple, the disadvantage is that it is easy to cause pollution

Method used

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  • Production technology for extracting berberine from cortex phellodendri and coptidis rhizoma through complex enzyme method
  • Production technology for extracting berberine from cortex phellodendri and coptidis rhizoma through complex enzyme method
  • Production technology for extracting berberine from cortex phellodendri and coptidis rhizoma through complex enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Combined with the properties of β-glucanase, xylanase, phytase, and cutinase, three factors are selected, namely enzymolysis temperature, enzymolysis pH, and enzyme addition (the ratio of medicinal materials to enzymes). A three-level three-factor orthogonal table was designed, and the value ranges of the three factors were: enzymatic hydrolysis temperature (30°C, 40°C, 50°C); enzymatic hydrolysis pH (3.0, 4.5, 6.0); enzyme ratio (herb: β- Glucanase: xylanase: phytase: cutinase = 500: 2-6: 0.1-0.5: 0.5-1: 2-6), orthogonal table 2-3.

[0051] Table 2 Enzyme hydrolysis factor level table

[0052]

[0053] Table 3 Orthogonal experiment table

[0054]

[0055] (1) Crush Cortex Phellodendri (Coptis Rhizoma) until it becomes granular, and pass through a 20-mesh sieve.

[0056] (2) Weigh 200g of pulverized Chinese medicinal materials, add various enzymes according to the conditions designed by the orthogonal experiment, add water and soak for 60 minutes, adjust the pH ...

Embodiment 2

[0066] According to the best result of embodiment 1 test, enzymolysis temperature: 50 ℃; Enzymolysis pH value: 6.0, add enzyme ratio (cork cork: β-glucanase: xylanase: phytase: cutinase=500 :4:0.25:0.5:4).

[0067] (1) Weigh 200g of Phellodendron cortex, crush it, pass through a 20-mesh sieve, add 400g of water and soak for 60min, add 1.6g, 0.1g, and 0.2g of β-glucanase, xylanase, phytase, and cutinase to it , 1.6g, hydrochloric acid solution to adjust the pH to 6.0, enzymolysis at 50°C for 6h, and boiling for 4min to inactivate the enzyme.

[0068] (2) Add 1400g of water, adjust the pH to 2.5 with dilute hydrochloric acid, extract at 90°C for 70 minutes, filter while hot, and collect the filtrate.

[0069] (3) Add 1500 mL of 70% ethanol to the filter residue, extract at a temperature of 90° C. for 90 min, extract twice, concentrate the filtrate, and adjust the pH to 3 with hydrochloric acid;

[0070] (4) Add saturated NaCl solution, stir until a yellow product flocs out, fi...

Embodiment 3

[0075] (1) Weigh 200g of Cortex Phellodendri, crush it, pass through a 20-mesh sieve, soak in water for 60min, add β-glucanase, xylanase, phytase, cutinase each 1.6g, 0.1g, 0.2g, 1.6g, adjust the pH to 6.0 with hydrochloric acid solution, enzymatically hydrolyze at 50°C for 3h, and boil for 4min to inactivate the enzyme.

[0076] (2) Add 1320g of water, adjust the pH to 3 with dilute hydrochloric acid, extract at 90°C for 70 minutes, filter while hot, and collect the filtrate.

[0077] (3) Add 1600 mL of 70% ethanol to the filter residue, extract at 75°C for 60 min, extract twice, concentrate the filtrate, and adjust the pH to 3 with hydrochloric acid;

[0078] (4) Add saturated NaCl solution, stir until a yellow product flocs out, filter with suction, dry, store overnight at 4°C, filter with suction, and dry to obtain the final product.

[0079] (5) Weigh the product weight after drying and cooling, calculate the yield, yield=(product quality / medicine quality)×100%, measure ...

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Abstract

The invention discloses a production technology for extracting berberine from cortex phellodendri and coptidis rhizoma through a complex enzyme method.The production technology comprises the following steps that1, the medicinal material cortex phellodendri or coptidis rhizoma is smashed, and water is added to the powder for soaking; 2, beta-dextranase, xylanase, phytase and cutinase are added into the mixture, the pH is adjusted with diluted hydrochloric acid, enzymolysis and boiling are conducted, enzymes are subjected to inactivation, and enzymatic hydrolysate is obtained; 3, water is added, the pH is adjusted with diluted hydrochloric acid, extraction is conducted, hot filtration is conducted, and filtrate is collected; 4, ethyl alcohol is added into filter residues, and digestion is conducted; 5, filtrates are combined, concentration is conducted, the pH is adjusted with diluted hydrochloric acid, cooling is conducted, a saturated NaCl solution is added, overnight preservation is conducted at 4 DEG C, suction filtration is conducted, drying is conducted, and berberine is obtained.The production technology for extracting berberine from cortex phellodendri and coptidis rhizoma through the complex enzyme method is simple in method, high in product yield and purity, low in cost and free of environmental pollution.

Description

technical field [0001] The invention relates to a production process for extracting berberine from phellodendron cortex and coptis chinensis by a compound enzyme method, and belongs to the field of traditional Chinese medicine pharmacy, pesticide, veterinary drug, feed or raw material processing. Background technique [0002] Cortex Phellodendron is the dry bark of Phellodendron chinense Schneid or Phellodendron amurense Rupr in Rutaceae. The former is commonly called "Sichuan Phellodendron", and the latter is commonly called "Guan Phellodendron". The main component of cortex cortex is berberine (Berberine), which also contains phellodendrine (Phellodendrine), magnoflorine (Magnoflorine), jatrorrhizine (Jatrorrhizine), palmate tetrandrine (Palmatine), N-methyl large Candicine, Menisperine and other alkaloids. In addition, it also contains Obaculactone, Obacunone, Obacunonic acid, 7-Dehydrostigmasterol, β-Sitosterol, and Campesterol ), Lumicaeruleicacid, Dictamno-lide. Co...

Claims

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Application Information

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IPC IPC(8): C12P17/18
CPCC12P17/18
Inventor 葛喜珍温玉博田平芳刘红梅李可意
Owner 天津尚美化妆品有限公司
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