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Method for preparing ultra-thin porous lamination gradient composite support of tissue engineering

A gradient tissue and composite scaffold technology, applied in the field of biomedical materials, can solve the problems of inability to repair joint osteochondral damage, poor bonding strength, poor pore connectivity, etc., achieve controllable degradation rate, good biocompatibility, mechanical good performance effect

Inactive Publication Date: 2010-01-20
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In such a preparation process, the bonding strength between layers is poor, the connectivity of pores is poor, and each porous layer must have a certain thickness to maintain its structure, shape and certain strength.
According to relevant literature reports, the thickness of the porous single-layer composite scaffold cannot be less than 1.5 mm (Anna Tampieri et al., Design of graded biomimetic osteochondral composite scaffolds, Biomaterials, 2008, 29: 3539-3546), therefore, according to the above-mentioned conventional preparation process, The thickness of the porous double-layer composite scaffold cannot be less than 3 mm, and the overall thickness of the porous laminated scaffold with three or more layers cannot reach the 2-4 mm required for repairing articular osteochondral damage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A zinc hydroxyapatite / polylactic acid bilayer composite scaffold with a thickness of 1.5 mm was prepared. The mold used was a cylindrical flat-bottomed glass vessel with an inner diameter of 60 mm. The organic material used is L-polylactic acid (PLLA, molecular weight 50000); the inorganic material used is zinc-containing nano-hydroxyapatite (nano-Zn-HA, needle-shaped particles, length 80-120nm, width 20-50nm); the solvent used is Acetone (C 3 h 6 O); the porogen used is sodium chloride (NaCl, divided into two types with a particle size of 50-200 μm and a particle size of 200-300 μm). The thickness of the laminated stent to be prepared is 1.5 mm, wherein the thickness of the first layer (lower layer) of the stent is 0.5 mm, and the thickness of the second layer (upper layer) is 1 mm. The composition of the first layer of the scaffold is a mixture of nano-Zn-HA and PLLA, and the mass ratio of nano-Zn-HA to PLLA is 30:70; the composition of the second layer of the scaf...

Embodiment 2

[0034] A three-layer composite scaffold of hydroxyapatite / polylactic acid-glycolic acid with a thickness of 3 mm was prepared. The mold used was a cylindrical flat-bottomed glass container with an inner diameter of 40 mm. The organic material used is polylactic acid-glycolic acid (PLGA, molecular weight 100000, LA:GA=50:50); the inorganic material used is nano-hydroxyapatite (nano-HA, needle-shaped particles, 100-150nm long, 20-20nm wide 50nm); the solvent used was dichloromethane (CH 2 Cl 2 ); the porogen used is sodium chloride (NaCl, particle size 200-300 μm). The thickness of the laminated stent to be prepared is 3mm, and the thickness of each layer is 1mm. The composition of the first layer (bottom layer) of the scaffold is a mixture of nano-HA and PLGA, the mass ratio of nano-HA to PLGA is 40:60; the composition of the second layer (middle layer) of the scaffold is a mixture of nano-HA and PLGA, nano-HA The mass ratio of HA to PLGA is 20:80; the composition of the th...

Embodiment 3

[0041] A four-layer composite scaffold of hydroxyapatite / polylactic acid-glycolic acid / polycaprolactone with a thickness of 4.5 mm was prepared. The mold used was a cylindrical flat-bottomed glass container with an inner diameter of 40 mm. The organic material used is polylactic acid glycolic acid (PLGA, molecular weight 100000, LA:GA=50:50) and polycaprolactone (PCL, molecular weight 70000); the inorganic material used is nano-hydroxyapatite (nano-HA, needle-like particles, length 100-150nm, width 20-50nm); the solvent used is chloroform (chloroform, CHCl 3 ) and tetrahydrofuran (tetrahydrofuran, THF); the porogen used is sodium chloride (NaCl, divided into two types with a particle size of 50-200 μm and 200-300 μm). The thickness of the laminated stent to be prepared is 4.5mm, wherein the thickness of the first layer (bottom layer) of the stent is 1mm, the composition is a mixture of nano-HA and PCL, and the mass ratio of nano-HA to PCL is 40:60; the second layer The thick...

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Abstract

The invention discloses a method for preparing an ultra-thin porous lamination gradient composite support of tissue engineering, belonging to the field of biomedicine material. The preparation method comprises the following steps: (1) determining materials and structures of a support to be prepared according to the condition of repair and defection; (2) preparing each layer of the support, waiting for the volatilization of a solvent, and gradually drying slurry for forming; (3) placing a formed object to a ventilation place, and demoulding the formed object after the residual solvent is completely volatilized; and (4) taking out the formed object and dry to obtain the ultra-thin porous lamination gradient composite support of the tissue engineering. The ultra-thin porous lamination composite support, the thickness of which is suitable for articular cartilage repair, can be prepared by using the method. The prepared support has favorable mechanical property, favorable biocompatibility and controllable degradation rate, is suitable for co-culture of osteoblast and chondrocyte and compound of specific growth factors, and can repair the synthetic defection of the joint cartilage tissue and the cartilage / bone tissue.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method of a porous laminated tissue engineering composite support for articular osteochondral defect repair. Background technique [0002] Articular cartilage defect is a very common disease. Due to the special tissue structure and biological characteristics of articular cartilage, although there are many known methods for treating articular cartilage defect, all of these methods cannot achieve satisfactory repair results. The emergence of tissue engineering technology has made it possible to repair cartilage tissue defects. The basic principle is to inoculate cartilage tissue cells isolated and cultured in vitro onto a three-dimensional scaffold with a certain spatial structure and good biocompatibility, supplemented with special growth factors, and then culture the cell-scaffold complex in vitro for a certain period of time. After a period of time ...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/54A61L27/56
Inventor 张胜民仇志烨黄浩周磊王深琪
Owner HUAZHONG UNIV OF SCI & TECH
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