Method for rapidly screening and quantifying antioxidant active components in traditional Chinese medicine on line

Abstract

The invention belongs to the field of medicine, and particularly relates to a method for screening and quantifying ABTS<+>-CE-DAD of antioxidant active components in traditional Chinese medicine. Taking Shuxuening as an example, specific to the aim of verifying the feasibility of the method, nine types of antioxidant active components in Shuxuening are screened and determined. In an experimental process, reaction conditions such as the pH value and phosphate concentration of a buffer solution, the concentrations of sodium dodecyl sulfate and beta-cyclodextrin, the content of acetonitrile, the temperature and the voltage are optimized. Under the same condition, ABTS+ and a Shuxuening injection sample are sampled in sequence, and components with antioxidant activity are reacted with ABTS+ and detected separately. Meanwhile, the certain detected antioxidant active components can be quantified by the determined conditions, and the total content of the active components in a sample is found to be linear with total antioxidant activity. As proved by the result of methodology, the method can be used for screening active compounds in traditional Chinese medicine on line, is easy, convenient, reliable, low in energy consumption, environmentally friendly, and easy for automation.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method capable of screening and quantifying antioxidant active ingredients in traditional Chinese medicines. Background technique [0002] In recent years, scientists have increasingly believed that reactive oxygen species can damage the main structure of cells, especially lipids and DNA, and then cause cell necrosis, promote aging and even cause cancer. The antioxidant activity can scavenge oxygen free radicals, thereby inhibiting senile diseases, various cardiovascular and cerebrovascular diseases and neurodegenerative diseases, and can prevent the occurrence of cancer and delay aging. Natural products, especially traditional Chinese medicine, have been proven to contain a large number of antioxidant active ingredients. Therefore, we should regard traditional Chinese medicine as a key resource for finding antioxidants. [0003] Ginkgo biloba, a traditional Chin...

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Problems solved by technology

However, since the analytical instrument is still in the liquid phase, the amount of organic reagents used in the whole experiment is still very large.

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