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Application of 2,6-di-tert-butyl-4-methylphenol as plant activator on apple fruits

A technology of di-tert-butyl and p-cresol, applied in plant growth regulators, plant growth regulators, applications, etc., can solve the problems of difficulty in controlling apple fruit diseases, resistance to chemical pesticide residues, and high cost, and achieve sustainable development. The effect of broad-spectrum characteristics, low cost and simple method

Active Publication Date: 2018-04-10
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at problems such as difficult prevention and control of apple fruit diseases in current agricultural production, high cost, increasingly prominent chemical pesticide residues and drug resistance, etc., and provides a method for inducing apple fruit disease resistance to effectively prevent and control apple fruit diseases

Method used

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  • Application of 2,6-di-tert-butyl-4-methylphenol as plant activator on apple fruits
  • Application of 2,6-di-tert-butyl-4-methylphenol as plant activator on apple fruits
  • Application of 2,6-di-tert-butyl-4-methylphenol as plant activator on apple fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1.1 Effect of 2,6-di-tert-butyl-p-cresol on mycelia growth of pathogenic fungus in apple

[0033] Prepare PDA medium, add 2,6-di-tert-butyl-p-cresol aqueous solution to it, so that the final concentration is 0.1mmol L -1 and 0.2mmol·L -1 , in the above-mentioned PDA medium, respectively inoculate the good apple ring spot pathogen Botryosphaeria dothidea (Botryosphaeria dothidea) of activation, Botrytis cinerea (Botrytis cinerea) of gray mold pathogenic bacteria, Penicillium expansum (Penicillium expansum) of penicillium pathogenic bacteria , Anthracnose pathogen Glomerellacingulata, Brown rot pathogen Monilinia fructicola, Spot disease pathogen Acremonium sclerotigenum, Mold core disease pathogen Trichothecium roseum, Alternaria alternate and Fusarium spp., the above-mentioned bacteria are common pathogenic bacteria in this field, were cultured at 25°C for 3-5 days in constant temperature and dark, and the colony diameter was measured.

[0034]The above-mentioned PDA ...

Embodiment 2

[0041] Control effects of different concentrations of 2,6-di-tert-butyl-p-cresol on apple fruit diseases:

[0042] Configure 0.1mmol·L -1 and 0.2mmol·L -1 Aqueous solution of 2,6-di-tert-butyl-p-cresol, apple ring spot pathogen Botryosphaeria dothidea, gray mold pathogen Botrytiscinerea, penicillium pathogen Penicillium expansum ), anthracnose pathogen Glomerella cingulata, brown rot pathogen Monilinia fructicola, spot disease pathogen Acremonium sclerotigenum, mold core pathogen pink Trichothecium roseum), Alternaria alternate and Fusarium spp. were activated and cultured on PDA medium for 3 days, and fungus cakes with a diameter of 5 mm were collected from the edge of the colonies for later use.

[0043] Select healthy Fuji apples of uniform size, wash them with running water, sterilize their surfaces with 75% alcohol, dry them at room temperature, and stab them at the equator of the apples. Each wound is about 0.3mm in diameter and 1.0mm deep. The above 0.1mmol·L -1 and...

Embodiment 3

[0049] The control effect of 2,6-di-tert-butyl-p-cresol varies with the interval of use:

[0050] According to the method in Example 2, the apple fruit is processed, and after the wound is caused by acupuncture, 0.1mmol L -1 The 2,6-di-tert-butyl-p-cresol aqueous solution is evenly sprayed on the fruit until there are water droplets dripping, and it is dried at room temperature. After spraying, 6h, 12h, 24h, 48h, 72h and 96h, the apples are inoculated at the stab wound. Bacteria cakes of Botryosphaeria dothidea and Botrytiscinerea, and the control effect of 2,6-di-tert-butyl-p-cresol with the interval time after inoculation 3 days and 5 days after inoculation were respectively measured.

[0051] The result is as image 3 and Figure 4 Shown:

[0052] image 3 It is the control effect of 2,6-di-tert-butyl-p-cresol on the pathogenic bacteria of apple ring spot with the change of application interval, which is determined by image 3 Known: 0.1mmol·L -1 After treatment with ...

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Abstract

The invention discloses an application of 2,6-di-tert-butyl-4-methylphenol as a plant activator on apple fruits, and belongs to the technical field of plant induced disease resistance. A 2,6-di-tert-butyl-4-methylphenol solution with the concentration of 0.1 mmol*L<-1>-0.2 mmol*L<-1> can be used for inducing apple fruit disease resistance and preventing and treating apple diseases. The 2,6-di-tert-butyl-4-methylphenol solution has no isolated bactericidal or bacteriostatic effect in itself, can induce plant disease resistance under living body conditions, and the drug resistance produced by induction has the characteristics of long persistence and broad spectrum, and 2,6-di-tert-butyl-4-methylphenol is environmentally friendly and safe and does not suitably produce drug resistance. 2,6-di-tert-butyl-4-methylphenol has the advantages of simple use method, low concentration, low amount and low cost, and can also improve the activity of defense enzymes in plants and total antioxidant capacity.

Description

technical field [0001] The invention belongs to the technical field of plant induced disease resistance, and in particular relates to the application of 2,6-di-tert-butyl-p-cresol as a plant disease resistance activator on apple fruits. Background technique [0002] Apples are rich in carbohydrates, vitamins and inorganic salts, and are an important source of nutrition and material basis for people. As an important economic crop and export fruit in my country, apples have the largest planting area and output in the world. However, the export rate of apples is less than 3% of the total output, and the market share in the world is extremely disproportionate to my country's status as a major apple producing country. Apple diseases, especially fruit diseases, are an important factor causing this phenomenon. Diseases caused by fungal infections in various links of apple production have brought huge losses to people. [0003] Apple fruit diseases mainly include: apple ring spot (B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N31/08A01P21/00A01P3/00
CPCA01N31/08
Inventor 王彩霞孟璐璐张清明李桂舫李保华
Owner QINGDAO AGRI UNIV
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